In vitro antimicrobial activity of extracts and their fractions from three Eryngium L. species

• Autorzy: Kikowska M. , Dlugaszewska J. , Kubicka M.M. , Kedziora I. , Budzianowski J. , Thiem B.

Warianty tytułu

PL

Właściwości przeciwbakteryjne ekstraktów i ich frakcji trzech gatunków Eryngium L.

• Języki publikacji: EN

Abstrakty

EN

Introduction: Due to increasing resistance against antibiotics and antifungal agents, crude plant extracts, fractions, and isolated pure compounds became a new interest as antimicrobial agents. Objectives: The antimicrobial activity of methanolic extracts and fractions of Eryngium planum L., E. campestre L., and E. maritimum L. was evaluated against selected bacteria, yeast and mould, and compared in tested Eryngium species and in their organs. Methods: The antimicrobial activity was studied with use of broth microdilution method. The antibacterial (Staphylococcus aureus, Pseudomonas aeruginosa) and antifungal (Candida albicans, Aspergillus niger) activity of selected extracts and fractions compared with the reference substance was expressed by Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal/Fungicidal Concentration (MBC/MFC).The extract and fraction compounds were identified on the basis of TLC examination. Results: The saponin-phenolic acid fractions of E. maritimum and E. planum and a saponin fraction of E. planum showed the highest activity against S. aureus (MIC = 1–2.5 mg·ml-1). The growth of C. albicans was inhibited by methanolic extract of E. planum cell suspension culture (MIC = 7.8 mg·ml-1). Conclusion: The antimicrobial activity depends on the Eryngium species, tested biomass, and microorganism.

PL

Wstęp: Z powodu rosnącej oporności na antybiotyki i środki przeciwgrzybicze rośnie zainteresowanie ekstraktami roślinnymi, frakcjami i wyizolowanymi czystymi związkami jako środkami przeciwdrobnoustrojowymi. Cel: Badano aktywność przeciwdrobnoustrojową ekstraktów metanolowych i frakcji Eryngium planum L., E. campestre L. i E. maritimum L. w stosunku do wybranych bakterii, drożdżaka i grzyba pleśniowego oraz porównywano badane gatunki Eryngium i ich organy. Metody: Aktywność przeciwdrobnoustrojową badano z zastosowaniem metody seryjnych rozcieńczeń w bulionie. Aktywność przeciwbakteryjna (Staphylococcus aureus, Pseudomonas aeruginosa) i przeciwgrzybicza (Candida albicans, Aspergillus niger) wybranych ekstraktów i frakcji, w porównaniu z substancją referencyjną, wyrażona została za pomocą minimalnego stężenia hamującego (MIC) i minimalnego stężenia bakterio- lub grzybobójczego (MBC/MFC). Wyniki: Frakcje saponinowo-fenolokwasowe E. maritimum i E. planum oraz frakcja saponinowa E. planum wykazały najwyższą aktywność wobec S. aureus (MIC = 1–2,5 mg∙ml -1). Wzrost C. albicans był hamowany przez metanolowy ekstrakt zawiesiny komórkowej E. planum (MIC = 7,8 mg∙ml -1). Wnioski: Aktywność przeciwdrobnoustrojowa zależy od gatunku Eryngium, testowanej biomasy i mikroorganizmu.

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2016
• Tom: 62
• Numer: 2
• Opis fizyczny: p.67-77,ref.

Twórcy

autor

Kikowska M.

• Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medicinal Sciences, Sw.Marii Magdaleny 14, 61-861 Poznan, Poland

autor

Dlugaszewska J.

• Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medicinal Sciences, Swiecickiego 4, 60-781 Poznan, Poland

autor

Kubicka M.M.

• Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medicinal Sciences, Swiecickiego 4, 60-781 Poznan, Poland

autor

Kedziora I.

• Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medicinal Sciences, Sw.Marii Magdaleny 14, 61-861 Poznan, Poland

autor

Budzianowski J.

• Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medicinal Sciences, Sw.Marii Magdaleny 14, 61-861 Poznan, Poland

autor

Thiem B.

• Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medicinal Sciences, Sw.Marii Magdaleny 14, 61-861 Poznan, Poland

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Identyfikatory

DOI

10.1515/hepo-2016-0012