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2018 | 27 | 1 |
Tytuł artykułu

Redox status, lipid peroxidation and protein oxidation levels in small ruminants

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Redox status, lipid peroxidation and protein oxidative damage in blood samples of healthy goats and sheep were evaluated to show any possible correlation with daily milk yield or milk components, and between oxidant parameters and biomarkers of protein and lipid damage. Blood and milk samples were collected from fifty Maltese goats and forty Valle del Belice sheep. Redox status was assessed analysing the total oxidant status (TOS), the total antioxidant capacity (TAC), together with the nitric oxide radical (NO• ) metabolite (NOx) content. Oxidative damage of lipids and proteins was analysed as thiobarbituric acid reactive substances (TBARS) and advanced oxidation protein products (AOPP), respectively. As for the comparison of the assessed oxidative stress (OS) parameters in the two species, only AOPP appeared significantly different (P < 0.01). While no correlation was observed between daily milk yield or milk components and the assessed OS parameters in both species, several correlations were shown among the assessed OS parameters, with differences appearing between the two species. In particular, TBARS levels were related to TOS in goats (P < 0.001) and to NOx in sheep (P < 0.001). Levels of AOPP were dependent on NOx concentrations in sheep (P < 0.05) but they were correlated neither with TOS nor NOx in goats (P > 0.05). So, the observed differences between two examined species seem to suggest that OS parameters are species-specific and a panel of different measurements based on various analytical methods should be considered to dutifully evaluate the OS.
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  • Department of Sciences, University of Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, Italy
  • Department of Veterinary Science, University of Messina, Polo Universitario dell’Annunziata, 98168 Messina, Italy
  • Department of Veterinary Science, University of Messina, Polo Universitario dell’Annunziata, 98168 Messina, Italy
  • Experimental Zooprophylaxis Institute of Sicily “A. Mirri”, 90121 Palermo, Italy
  • Indipendent Agronomist, 85050 Baragiano (PZ), Italy
  • Department of Veterinary Science, University of Messina, Polo Universitario dell’Annunziata, 98168 Messina, Italy
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