The influence of associative pairing of visual stimulation and tail shock on somatostatin expression in mouse primary visual cortex

• Autorzy: Raciborska I. , Popiolkiewicz J. , Waleszczyk W.J.
• Języki publikacji: EN

Abstrakty

EN

BACKGROUND AND AIMS: Previous study showed that associative pairing involving monocular visual stimulation resulted in a significant increase of the density of cells expressing glutamic acid decarboxylase (GAD), γ-aminobutyric acid (GABA) synthesizing enzyme, in mouse primary visual cortex contralateral to the stimulated eye. The effect was attributed to the group of parvalbumin- (PV)-negative interneurons, since the density of PV-positive cells remained unaffected. The aim of the present study is to identify the group(s) of PV-negative GABA-ergic cells in mouse visual cortex that are mobilized by visual training combined with tail shock. METHODS: The same method of associative pairing (classical conditioning) involving monocular visual stimulation was used. We used nine young adult male mice, which were divided into two experimental groups: trained (5 mice) and naïve (4 mice) that served as a control. During three days lasting training, monocularly presented visual stimulus, drifting gratings of optimal spatial and temporal frequency (conditioned stimulus, CS), was coupled with electric shock applied to the tail (unconditioned stimulus, UCS) at the end of visual stimulation. Twenty four hours after the training (CS+UCS), mouse brains were subject to immunohistochemistry. The brain slices were photographed through fluorescent microscope and analyzed quantitatively to determine the amount of fluorescence using Fiji ImageJ software. RESULTS: Monocular visual training combined with tail shock resulted in a significant increase of GAD- and SOM-positive puncta in the contralateral primary visual cortex of (CS+UCS) mice in comparison to naive group. CONCLUSIONS: Our results confirm earlier finding of the upregulation of cortical GABA-ergic system by classical conditioning involving sensory stimulation and indicate on engagement of SOMpositive interneurons.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.S97-S98

Twórcy

autor

Raciborska I.

• Nencki Institute of Experimetal Biology, Polish Academy of Sciences, Warsaw, Poland

autor

Popiolkiewicz J.

• Nencki Institute of Experimetal Biology, Polish Academy of Sciences, Warsaw, Poland

autor

Waleszczyk W.J.

• Nencki Institute of Experimetal Biology, Polish Academy of Sciences, Warsaw, Poland