Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants

• Autorzy: Pilarska M. , Popielarska-Konieczna M. , Slesak H. , Kozieradzka-Kiszkurno M. , Goralski G. , Konieczny R. , Bohdanowicz J. , Kuta E.
• Języki publikacji: EN

Abstrakty

EN

Helianthus tuberosus is economically important species. To improve characters of this energetic plant via genetic modification, production of callus tissue and plant regeneration are the first steps. A new, potentially energetic cultivar Albik was used in this study to test callus induction and regeneration. Callus was produced on leaves, petioles, apical meristems and stems from field-harvested plants but was totally non-morphogenic. Its induction started in the cortex and vascular bundles as confirmed by histological analysis. The surface of heterogeneous callus was partially covered with a membranous extracellular matrix surface network visible in scanning and transmission electron microscopies. The results clearly indicate that: (i) the morphogenic capacity of callus in topinambur is genotype dependent, (ii) cv. Albik of H. tuberosus proved recalcitrant in in vitro regeneration, and (iii) extracellular matrix surface network is not a morphogenic marker in this cultivar.

Słowa kluczowe

EN

callogenesis; extracellular matrix; surface network; in vitro culture; non-morphogenic callus; Helianthus tuberosus; Albik cultivar; explant; transmission electron microscopy

• Wydawca: -
• Czasopismo: Acta Societatis Botanicorum Poloniae
• Rocznik: 2014
• Tom: 83
• Numer: 1
• Opis fizyczny: p.67-73,fig.,ref.

Twórcy

autor

Pilarska M.

• Department of Plant Cytology and Embryology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland

autor

Popielarska-Konieczna M.

• Department of Plant Cytology and Embryology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland

autor

Slesak H.

• Department of Plant Cytology and Embryology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland

autor

Kozieradzka-Kiszkurno M.

• Department of Plant Cytology and Embryology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland

autor

Goralski G.

• Department of Plant Cytology and Embryology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland

autor

Konieczny R.

• Department of Plant Cytology and Embryology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland

autor

Bohdanowicz J.

• Department of Plant Cytology and Embryology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland

autor

Kuta E.

• Department of Plant Cytology and Embryology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland

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Identyfikatory

DOI

10.5586/asbp.2014.009