PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2016 | 587 |
Tytuł artykułu

Czy białko może być obecne w glebie nawet 20 lat? Ocena zawartości ogólnych i łatwoekstrahowalnych glomalin w archiwalnych próbach glebowych

Treść / Zawartość
Warianty tytułu
EN
Can soil proteins be present in the soil even 20 years? Assessment of total and easy extractable glomalin contents in archival samples of soil
Języki publikacji
PL
Abstrakty
PL
Glomaliny to glikorpoteiny grzybowe, które są wyjątkowo odporne na degradację (obecne w glebie nawet od 10 do 30 lat) oraz trudno rozpuszczalne w wodzie. Celem niniejszej pracy była ocena zawartości ogólnych i łatwoekstrahowalnych glomalin w archiwalnych próbkach glebowych. Do badań wybrano 15 próbek glebowych z doświadczeń polowych z 1994 roku dotyczących uprawy jęczmienia jarego. Badane gleby pochodziły z różnych regionów Polski oraz reprezentowały różne typy gleb. Gleby charakteryzowały się także dużym zróżnicowaniem właściwości fizykochemicznych i biologicznych. Przeprowadzone w niniejszej pracy badania wykazały obecność glomalin we wszystkich próbkach glebowych. Stężenie ogólnych i łatwoekstrahowalnych glomalin zależało głównie od typu gleby. Obecność glomalin w badanych glebach potwierdza hipotezę, iż białka te są szczególnie odporne na degradację i mogą pozostać w niezmienionej formie w glebie przez wiele lat.
EN
Glomalines are thermostable, water-insoluble glycoproteins abundantly produced by Glomus fungi and tends to accumulate in the soil, that surrounds soil aggregates and protects them from destroying. Glomalines are especially resistant to destruction, hard to dissolve in water, while very easy dissolve in high temperature (121°C) in citrate buffer with neutral or alkali pH. These proteins have very unique physico-chemical properties performing a fundamental role in making soil structure. The content of glomalin in soil particles is significantly correlated with their stability (water resistance). It is possible that glomalin is involved in the formation of hydrophobic properties of soil, improving water and air relations in soil. Glomalines are mainly located on the surface of hyphae and spores of AM (arbuscular mycorrhizal) fungi and a hydrophobic substances covers and protects it from the degradation by other microorganisms. It also increases the stiffness and the durability of the cell of fungi facilitating their distribution in the soil. Production and distribution of glomalin in soil involves many factors, both related to the physico-chemical properties of soil and plant and fungal species. The structure of soil and its chemical properties have a significant effect on the growth of mycelium, and therefore the glomalin content. Glomalins are very resistant to degradation. As aging hyphae outside the root and the roots of plants, biomass mycelium of AMF fungi is degraded – but not glomalin. This protein is unchanged is able to survive in the soil even decades. Glomalins commonly occur in different types of soils. The aim of the study was to demonstrate the presence of total and easy extractable glomalins in archival samples of soil from the collection of soil Department of Microbiology IUNG-PIB in Puławy. The study selected 20 soil samples from field trials in 1994 with the cultivation of barley. The soil samples survey from various Polish regions and represented different types of soils. Soils were characterized by different physical, chemical and biological properties. The studies carried out in this paper have shown the contents of glomalin in all soil samples. The concentration of total and easily extracted glomalin depend mainly on the type of soil. The presence of glomalin in soils confirms the hypothesis that these proteins are particularly resistant to degradation and can remain unchanged in the soil even decades.
Słowa kluczowe
PL
Wydawca
-
Rocznik
Tom
587
Opis fizyczny
s.13-21,rys.,tab.,bibliogr.
Twórcy
autor
  • Instytut Uprawy Nawożenia i Gleboznawstwa w Puławach - Państwowy Instytut Badawczy
  • Instytut Uprawy Nawożenia i Gleboznawstwa w Puławach - Państwowy Instytut Badawczy
Bibliografia
  • Bradford M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.
  • Cucci G., Lacolla G., Pagliai M., Vignozzi N., 2015. Effect of reclamation on the structure of silty-clay soils irrigated with saline-sodic waters. Int. Agrophys. 29, 23-30.
  • Driver J.D., Holben W.E., Rillig M.C., 2005. Characterization of glomalin as a hyphal wall component of arbuscular mycorrhizal fungi. Soil Biol. Biochem. 37, 101–106.
  • Fakom R., Adamou S., Teugwa M.C., Begoude Boyogueno A.D., Nana W.L., Ngonkeu M.E.L., Tchameni N.S., Nwaga D., Tsala Ndzomo G., Amvam Zollo P.H., 2012. Glomalin related soil protein, carbon, nitrogen and soil aggregate stability as affected by land use variation in the humid forest zone of south Cameroon. Soil Till. Res. 120, 69–75.
  • Gałązka A., 2013. Charakterystyka glomalin i oddziaływania różnych systemów uprawy na ich zawartość w glebie. Polish J. Agronom. 15, 75–82.
  • Gałązka A., Gawryjołek K., 2015. Glomalina – glikoproteina produkowana przez grzyby mykoryzy arbuskularnej. Postep Mikrobiol. 54 (3), 331–343.
  • Gillespie A.W., Farrell R.E., Walley F.L., Ross A.R.S., Leinweber P., Eckhardt K.U., Regier T.Z., Blyth R.I.R., 2011. Glomalin-related soil protein contains non-mycorrhizal-related heat-stable proteins, lipids and humic materials. Soil Biol. Biochem. 43, 766–777.
  • Guo H., He X.L., Li Y.P., 2012. Spatial distribution of arbuscular mycorrhiza and glomalin in the rhizosphere of Caragana korshinskii Kom. in the Otindag sandy land, China. Afr. J. Microbiol. Res. 6, 5745–5753.
  • Koide R.T., Peoples M.S., 2013. Behavior of Bradford-reactive substances is consistent with predictions for glomalin. Appl. Soil Ecol. 63, 8–14.
  • Król M., 1997. Drobnoustroje ryzosfery jęczmienia jarego. ISBN 83-85725-59-8, H(13). Rozprawa habilitacyjna.
  • Nichols K.A., 2004. Characterization of glomalin a glycoprotein produced by arbuscular mycorrhizal fungi. Agriculture, Soil Sci. 81, 123–129.
  • Purin S., Rillig M.C., 2007. The arbuscular mycorrhizal fungal protein glomalin: Limitations, progress, and a new hypothesis for its function. Pedobiologia 51, 123–130.
  • Rillig M.C., 2004. Arbuscular mycorrhizae and terrestrial ecosystem processes. Ecol. Lett. 7, 740–754.
  • Rillig M.C., Steinberg P.D., 2002. Glomalin production by an arbuscular mycorrhizal fungus: a mechanism of habitat modification? Soil Biol. Biochem. 34, 1371–1374.
  • Schindler F.V., Mercer E.J., Rice J.A., 2007. Chemical characteristics of glomalin-related soil protein (GRSP) extracted from soils of varying organic matter content. Soil Biol. Biochem. 39, 320–329.
  • Wang S., Wu Q.-S., He X.-H., 2015. Exogenous easily extractable glomalin-related soil protein promotes soil aggregation, relevant soil enzyme activities and plant growth in trifoliate orange. Plant Soil Environ. 61 (2), 66–71.
  • Wright S.F., Anderson R.L., 2000. Aggregate stability and glomalin in alternative crop rotations for the central Great Plains. Biol. Fert. Soils 31, 249–253.
  • Wright S.F., Franke-Synder M., Morton J.B., Upadhyaya A., 1996. Time course study and partial characterization of a protein on hyphae of arbuscular mycorrhizal fungi during active colonization of roots. Plant Soil 181, 193–203.
  • Wright S.F., Upadhyaya A., 1996. Extraction of an abundant and unusual protein from soil and comparison with hyphal protein from arbuscular mycorrhizal fungi. Soil Sci. 161, 575–586.
  • Wu Q.S., Li Y., Zou Y.N., He X.H., 2015. Arbuscular mycorrhiza mediates glomalin-related soil protein production and soil enzyme activities in the rhizosphere of trifoliate orange grown under different P levels. Mycorrhiza 25, 121–130.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-b8e5f441-ea29-4083-b794-d940e16dfd28
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.