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2011 | 33 | 6 |

Tytuł artykułu

Effects of explant types and media salt strength on growth and secondary metabolite accumulation in adventitious roots of Periploca sepium Bunge

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
In order to study the capabilities of Periploca sepium adventitious root induction in different types of explants, we selected leaves, roots and stems with or without buds. The growth of adventitious roots and periplocin content in these roots were determined. In order to investigate the suitable media salt strength, we cultured the adventitious roots in different salt strength (0.25, 0.50, 1.0, 1.5, 2.0) of Murashige and Skoog (MS) media supplemented with 1 mg/l indole butyric acid (IBA) and 30 g/l sucrose. The results showed that both leaf and root explants were proven suitable for the adventitious root induction; however, the stems could hardly induce adventitious roots no matter whether the stems had buds or not. Further studies reported that adventitious root proliferation and periplocin production derived from root explants were higher than those derived from leaf explants. So the root explants were the optimum explants for adventitious root induction, growth and periplocin production. The salt strength experiment showed that with the increasing salt strength (1.0–2.0 MS), adventitious root growth decreased significantly, as well as periplocin content in comparison with lower (0.25–0.5 MS) salt strength media.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

33

Numer

6

Opis fizyczny

p.2447-2452,fig.,ref.

Twórcy

autor
  • School of Traditional Chunese Materia Medica, Tianjin University of Traditional Chinese Medicine, No. 312 Anshan West Street, Nankai District, Tianjin 300193, People's Republic of China
autor
  • School of Pharmaceutical Science and Technology, Tianjin University, No. 92 Weijin Road, Nankai District, Tianjin 300072, People's Republic of China
autor
  • School of Pharmaceutical Science and Technology, Tianjin University, No. 92 Weijin Road, Nankai District, Tianjin 300072, People's Republic of China
autor
  • Institute of Biological Engineering of Traditional Chinese Medicine, Tianjin University of Science and Technology, Tianjin 300457, People's Republic of China

Bibliografia

  • Baque MA, Lee EJ, Paek KY (2010) Media salt strength induced changes in growth, physiology and secondary metabolite content in adventitious roots of Morinda citrifolia: the role of antioxidant enzymes and phenylalanine ammonia lyase. Plant Cell Rep 29:685–694
  • Chen YP, Li GX, Wu P et al (2005) The reason and precaution of clinically adverse reaction of coxtex periplocae radicis. Chin Hosp Pharm J 25(7):689–690
  • Cui XH, Charkrabarty D, Lee EJ, Paek KY (2010) Production of adventitious roots and secondary metabolites by Hypericum perforatum L. in a bioreactor. Bioresour Technol 101(12):4708–4716
  • Davis TD, Haissig BE (1994) Biology of adventitious root formation. Plenum Press, New York, pp 275–280
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  • Marks TR, Simpson SE (2000) Interaction of explant type and indole-3-butyric acid during rooting in vitro in a range of difficult and easy-to-root woody plants. Plant Cell Tissue Organ Cult 62(1):65–74
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  • Wu CH, Dewir YH, Hahn EJ, Paek KY (2006) Optimization of culturing conditions for the production of biomass and phenolics from adventitious roots of Echinacea angustifolia. J Plant Biol 49:193–199
  • Xu LQ, Lu HZ, Zhang YL (1998) Clinical observation on treatment of 147 cases of chronic congestive heart-failure by bei wujiapi mixture. Yunnan J Tradit Chin Med Materia Medica 19(4):29–31
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Bibliografia

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