Immunohistochemical assessment of renal tubular epithelial cell death after anthracyclines therapy

• Autorzy: Pedrycz A. , Boratynski Z. , Olesinski I. , Dudzinska M.
• Języki publikacji: EN

Abstrakty

EN

In the present study the quantitative and qualitative immunohistochemical examinations of rats' renal tubular epithelial cells were conducted to determine the presence of proteins involved in adriamycin-induced apoptosis, in which death signalling was produced through the mitochondrial pathway involving proteins: Bcl-2, BAX, Apaf-1, and caspases 9 and 3; the extrinsic pathway - proteins of caspase 8 and 3; and the endoplasmic reticulum pathway - caspases 12 and 3. Moreover, caspase 1 - one of the post- necrosis inflammation markers, was evaluated immunohistochemically. Adriamycin-induced apoptosis in the renal tubular cells occurred after 4 weeks and intensified after 7 weeks (an increase in reaction to the executing caspase 3). Irrespective of the time factor, it developed through the mitochondrial pathway (statistically-significant increase in reaction for, BAX, Apaf-1, caspase 9) and reticular pathway (statistically-significant increase in reaction for caspase 12). No increased reaction was observed for caspase 1.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2009
• Tom: 53
• Numer: 4
• Opis fizyczny: p.719-724,fig.,ref.

Twórcy

autor

Pedrycz A.

• Department of Histology and Embryology with Laboratory of Experimental Cytology, Medical University of Lublin, 20-080 Lublin, Poland

autor

Boratynski Z.

• Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-950 Lublin, Poland

autor

Olesinski I.

• Department of Neurology I, Neuropsychiatric Hospital, 20-442 Lublin, Poland

autor

Dudzinska M.

• Department of Lungs Diseases and Tuberculosis, Jan Bozy Hospital Lublin, 20-089 Lublin, Poland

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