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2009 | 31 | 2 |
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Purification and characterization of oxalate oxidase from wheat seedlings

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Oxalate oxidase (OxO, EC was purified to homogeneity from wheat (Triticum aestivum) seedlings by sequential thermal treatment, ultrafiltration, Sephadex G-100 gel filtration and affinity chromatography with concanavalin A. The enzyme was purified 66.11-fold with a recovery of 21.97%. It showed a subunit molecular mass of 32.6 kDa on SDS-PAGE and a native molecular mass of 170 kDa on Sephadex G-150 filtration, suggesting that it is a pentamer. The wheat OxO had a maximum activity at pH 3.5. Its Km for oxalate was 0.21 mM. Chemical modification revealed that cysteine, lysine and carboxylate residues were essential for OxO activity, whereas arginine, serine, threonine and tryptophane residues were not essential.
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  • College of Life Science, South China Agricultural University, 510642 Guangzhou, China
  • College of Life Science, South China Agricultural University, 510642 Guangzhou, China
  • Berna A, Bernier F (1997) Regulated expression of a wheat germin gene in tobacco: oxalate oxidase activity and apoplastic localization of the heterologous proteins. Plant Mol Biol 33:417–429. doi:10.1023/A:1005745015962
  • Bernier F, Berna A (2001) Germins and germin-like proteins: plant do-all proteins. But what do they do exactly? Plant Physiol Biochem 39:545–554. doi:10.1016/S0981-9428(01)01285-2
  • Bhattacharjee H, Rosen BP (2001) Structure-function analysis of the ArsA ATPase: contribution of histidine residues. J Bioenerg Biomembr 33:459–468. doi:10.1023/A:1012818920027
  • Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254. doi:10.1016/0003-2697(76)90527-3
  • Chitiboga J (1966) Purification and properties of oxalic acid oxidase. Arch Biochem Biophys 116:516–523. doi:10.1016/0003-9861 (66)90057-9
  • Delforge D, Devreese B, Dieu M, Delaive E, Beeumen JV, Remacle J (1997) Identification of lysine 74 in the pyruvate binding site of alanine dehydrogenase from Bacillus subtilis. Chemical modification with 2,4,6-trinitrobenzenesulfonic acid, N-succinimidyl 3-(2-pyridyldithio) propionate, and 59-(p-(fluorosulfonyl)benzoyl) adenosine. J Biol Chem 272:2276–2284. doi:10.1074/jbc. 272.4.2276
  • Dura MA, Flores M, Toldra F (2002) Purification and characterization of a glutaminase from Debaryomyces spp. Int J Food Microbiol 76:117–126. doi:10.1016/S0168-1605(02)00024-7
  • Gane PJ, Dunwell JM, Warwicker J (1998) Modeling based on the structure of vicilins predicts a histidine cluster in the active site of oxalate oxidase. J Mol Evol 46:488–493. doi:10.1007/ PL00006329
  • Hollenberg PF, Flashner M, Coon MJ (1971) Role of lysyl ε-amino groups in adenosine diphosphate binding and catalytic activity of pyruvate kinase. J Biol Chem 246:946–953
  • Jaikaran ASI, Kennedy TD, Dratewka-Kos E, Lane BG (1990) Covalently bonded and adventitious glycans in germin. J Biol Chem 265:12503–12512
  • Kotsira VP, Clonis YD (1997) Oxalate oxidase from barley roots: purification to homogeneity and study of some molecular catalytic, and binding properties. Arch Biochem Biophys 340:239–249. doi:10.1006/abbi.1997.9896
  • Kotsira VP, Clonis YD (1998) Chemical modification of barley root oxalate oxidase shows the presence of a lysine, a carboxylate, and disulfides, essential for enzyme activity. Arch Biochem Biophys 356:117–126. doi:10.1006/abbi.1998.0764
  • Laemmli UK (1970) Active-staining cleavage of structural protein during the assembly of the head of bacteriophage T₄. Nature 227:680–685. doi:10.1038/227680a0
  • Lane BG (1994) Oxalate, germin, and the extracellular matrix of higher plants. FASEB J 8:294–301
  • Lane BG (2000) Oxalate oxidases and differentiating surface structure in wheat: germins. Biochem J 349:309–321. doi:10.1042/0264-6021:3490309
  • Lane BG, Bernier F, Dratewka-Kos E, Shafai R, Kennedy TD, Pyne C et al (1991) Homologies between members of the germin gene family in hexaploid wheat and similarities between these wheat germins and certain Physarum spherulins. J Biol Chem 266:10461–10469
  • Lane BG, Cuming AC, Frégeau J, Carpita NC, Hurkman WH, Bernier F et al (1992) Germin isoforms are discrete temporal markers of wheat development: pseudogermin is a unique thermostable water-soluble oligomeric protein in ungerminated embryos and like germin in germinated embryos it is incorporated into cell wall. Eur J Biochem 209:961–969. doi:10.1111/j.1432-1033. 1992.tb17369.x
  • Lane BG, Dunwell JM, Ray JA, Schmitt MR (1993) Germin, a protein marker of early plant development, is an oxalate oxidase. J Biol Chem 268:12239–12242
  • Lerouge P, Cabanes-Macheteau M, Rayon C, Fischette-Laine AC, Gomord V, Faye L (1998) N-glycoprotein biosynthesis in plants: recent development and future trends. Plant Mol Biol 38:31–48. doi:10.1023/A:1006012005654
  • Liu W, Zhu WM, Wu DQ (1999) Chemical modification of xylanase from Trichosporon cutaneum shows the presence of carboxyl groups and cysteine residues essential for enzyme activity. J Protein Chem 18:677–686. doi:10.1023/A:1020658307787
  • Opaleye O, Rose RS, Whittaker MM, Woo EJ, Whittaker JW, Pickersgill RW (2006) Structural and spectroscopic studies shed light on the mechanism of oxalate oxidase. J Biol Chem 281:6428–6433. doi:10.1074/jbc.M510256200
  • Pritsa AA, Dimitrios A, Kyriakidis DA (2001) L-Asparaginase of Thermus thermophilus: purification, properties and identification of essential amino acids for its catalytic activity. Mol Cell Biochem 216:93–101. doi:10.1023/A:1011066129771
  • Requena L, Bornemann S (1999) Barley (Hordeum vulgare) oxalate oxidase is a manganese-containing enzyme. Biochem J 343:185–190. doi:10.1042/0264-6021:3430185
  • Subray SH, Ameeta RK, Krishna NG, Khan IM (1998) Catalytic thiol and carboxylate: role of cysteine and glutamic acid in the xylosidic activity of endoxylanase from Chainia sp. (NCL 82-5-1). Arch Biochem Biophys 355:153–159. doi:10.1006/abbi.1998.0729
  • Thordal-Christensen H, Zhang Z, Wei Y, Collinge DB (1997) Subcellular localization of H₂O₂ in plants. H₂O₂ accumulation in papillae and hypersensitive response during the barley powdery mildew interaction. Plant J 11:1187–1194. doi:10.1046/j.1365-313X.1997.11061187.x
  • Woo E, Dunwell JM, Goodenough PW, Pickersgill RW (1998) Barley oxalate oxidase is a hexameric protein related to seed storage proteins: evidence from X-ray crystallography. FEBS Lett 437:87–90. doi:10.1016/S0014-5793(98)01203-4
  • Woo E, Dunwell JM, Goodenough PW, Marvier AC, Pickersgill RW (2000) Germin is a manganese containing homohexamer with oxalate and superoxide dismutase activities. Nat Struct Biol 7:1036–1040. doi:10.1038/80954
  • Zhang Z, Yang J, Collinge DB, Thordal-Christensen H (1996) Ethanol increases sensitivity of oxalate oxidase assays and facilitates direct activity staining SDS gel. Plant Mol Biol Rep 14:266–272. doi:10.1007/BF02671662
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