Does allicin combined with vitamin B-complex have superior potentials than alpha-tocopherol alone in ameliorating lead acetate-induced Purkinje cell alterations in rats? An immunohistochemical and ultrastructural study

• Autorzy: Mustafa H.N. , Hussein A.M.
• Języki publikacji: EN

Abstrakty

EN

Background: The current article aims to explore the protective potentials of α-tocopherol alone and the combination of allicin and vitamin B-complex against lead-acetate neurotoxicity on the cerebellar cortex. Materials and methods: Forty rats were divided into four groups (n = 10). Group 1 was the control group; Group 2 received 10 mg/kg body weight (BW) of lead acetate; Group 3 was exposed to 10 mg/kg BW of lead acetate plus a combination of allicin (100 mg/kg BW) and vitamin B-complex (40 mg/kg BW); Group 4 was administered lead acetate (10 mg/kg BW) and α-tocopherol (100 mg/kg BW). The animals received the treatment for 60 days by oral gavage. All the groups were studied ultrastructurally and immunohistochemically with glial fibrillary acidic protein (GFAP). Results: The affected groups revealed shrunken and degenerated Purkinje cells with irregular nuclei. The cytoplasm comprised several lysosomes, unhealthy mitochondria, and dilated Golgi saccules. The myelinated nerve fibres demonstrated breaking of the myelin sheaths, apparent vacuoles, and broad axonal spaces. Immunohistochemically, there was a tremendous surge in GFAP-positive astrocytes in the lead acetate-treated group. These histological and ultrastructural variations were ameliorated by the administration of a-tocopherol and the combination of allicin and vitamin B complex. Moreover, an apparent decrease in the number of GFAP-positive astrocytes was obvious in the protected groups. Conclusions: Although both a-tocopherol and the combination of allicin and vitamin B-complex can be used as possible adjuvant therapies to ameliorate nervous system ailments attributable to lead acetate, α-tocopherol showed more protective potential. (Folia Morphol 2016; 75, 1: 76–86)

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2016
• Tom: 75
• Numer: 1
• Opis fizyczny: p.76-86,fig.,ref.

Twórcy

autor

Mustafa H.N.

• Anatomy Department, Faculty of Medicine, King Abdulaziz University, Building No.8 - P.O.Box: 80205 Jeddah, 21589, Saudi Arabia

autor

Hussein A.M.

• Anatomy Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia

Bibliografia

• 1. Abd El-Monem DD (2012) The modulating effect of melatonin against the genotoxicity of lead acetate. J Basic Appl Zoology, 65: 223–231.
• 2. Abdel Moneim AE, Dkhil MA, Al-Quraishy S (2011) The protective effect of flaxseed oil on lead acetate-induced renal toxicity in rats. J Hazard Mater, 194: 250–255.
• 3. Amadio S, D’Ambrosi N, Trincavelli ML, Tuscano D, Sancesario G, Bernardi G, Martini C, Volonté C (2005) Differences in the neurotoxicity profile induced by ATP and ATPgS in cultured cerebellar granule neurons. Neurochem Int, 47: 334–342.
• 4. Aminuddin M, Partadiredja G, Sari DC (2015) The effects of black garlic (Allium sativum L) ethanol extract on the estimated total number of Purkinje cells and motor coordination of male adolescent Wistar rats treated with monosodium glutamate. Anat Sci Int, 90: 75–81.
• 5. Araujo JA, Landsberg GM, Milgram NW, Miolo A (2008) Improvement of short-term memory performance in aged beagles by a nutraceutical supplement containing phosphatidylserine, Ginkgo biloba, vitamin E, and pyridoxine. Can Vet J, 49: 379–385.
• 6. Balk EM, Raman G, Tatsioni A, Chung M, Lau J, Rosenberg IH (2007) Vitamin B6, B12, and folic acid supplementation and cognitive functiona systematic review of randomized trials. Arch Int Med, 167: 21–30.
• 7. Bastian AJ (2011) Moving, sensing and learning with cerebellar damage. Curr Opin Neurobiol, 21: 596–601.
• 8. Baydas G, Nedzvetskii VS, Tuzcu M, Yasar A, Kirichenko SV (2003) Increase of glial fibrillary acidic protein and S-100B in hippocampus and cortex of diabetic rats: effects of vitamin E. Eur J Pharmacol, 462: 67–71.
• 9. Baydas G, Reiter RJ, Yasar A, Tuzcu M, Akdemir I, Nedzvetskii VS (2003) Melatonin reduces glial reactivity in the hippocampus, cortex, and cerebellum of streptozotocin-induced diabetic rats. Free Radic Biol Med, 35: 797–804.
• 10. Baydas G, Tuzcu M, Yasar A, Baydas B (2004) Early changes in glial reactivity and lipid peroxidation in diabetic rat retina: effects of melatonin. Acta Diabetol, 41: 123–128.
• 11. Bellé LP, De Bona KS, Abdalla FH, Pimentel VC, Pigatto AS, Moretto MB (2009) Comparative evaluation of adenosine deaminase activity in cerebral cortex and hippocampus of young and adult rats: effect of garlic extract (Allium sativum L) on their susceptibility to heavy metal exposure. Basic Clin Pharmacol Toxicol, 104: 408–413.
• 12. Burki TK (2012) Nigeria’s lead poisoning crisis could leave a long legacy. Lancet, 379: 792.
• 13. Chow CK (1990) Effect of dietary vitamin E and selenium on rats: Pyruvate kinase, glutathione peroxidase and oxidative damage. Nutr Res, 10: 183–194.
• 14. Costa LG, Aschner M, Vitalone A, Syversen T, Soldin OP (2004) Developmental neuropathology of environmental agents. Annu Rev Pharmacol Toxicol, 44: 87–110.
• 15. Cutler RR, Odent M, Hajj-Ahmad H, Maharjan S, Bennett NJ, Josling PD, Ball V, Hatton P, Dall’Antonia M (2009) In vitro activity of an aqueous allicin extract and a novel allicin topical gel formulation against Lancefield group B streptococci. J Antimicrob Chemother, 63: 151–154.
• 16. Das I, Saha T (2009) Effect of garlic on lipid peroxidation and antioxidation enzymes in DMBA-induced skin carcinoma. Nutrition, 25: 459–471.
• 17. Feeser VR, Loria RM (2011) Modulation of traumatic brain injury using progesterone and the role of glial cells on its neuroprotective actions. J Neuroimmunol, 237: 4–12.
• 18. FitzGerald JT, Gruener G, Mtui E (2011) Clinical Neuroanatomy and Neuroscience: Elsevier Health Sciences, UK.
• 19. Flora G, Gupta D, Tiwari A (2012) Toxicity of lead: a review with recent updates. Interdiscip Toxicol, 5: 47–58.
• 20. Gonzalez A, Pariente JA, Salido GM (2007) Ethanol stimulates ROS generation by mitochondria through Ca2+ mobilization and increases GFAP content in rat hippocampal astrocytes. Brain Res, 1178: 28–37.
• 21. Gu X, Wu H, Fu P (2013) Allicin attenuates inflammation and suppresses HLA-B27 protein expression in ankylosing spondylitis mice. Biomed Res Int, 2013: 171573.
• 22. Harrison DG, Guzik TJ, Lob HE, Madhur MS, Marvar PJ, Thabet SR, Vinh A, Weyand CM (2011) Inflammation, immunity, and hypertension. Hypertension, 57: 132–140.
• 23. Hernández-Fonseca JP, Rincón J, Pedreañez A, Viera N, Arcaya JL, Carrizo E, Mosquera J (2009) Structural and ultrastructural analysis of cerebral cortex, cerebellum, and hypothalamus from diabetic rats. Exp Diabetes Res, 2009: 329632.
• 24. Hossain MA, Russell JC, Miknyoczki S, Ruggeri B, Lal B, Laterra J (2004) Vascular endothelial growth factor mediates vasogenic edema in acute lead encephalopathy. Ann Neurol, 55: 660–667.
• 25. Huang F, Schneider J (2004) Effects of lead exposure on proliferation and differentiation of neural stem cells derived from different regions of embryonic rat brain. Neurotoxicology, 25: 1001–1012.
• 26. Hussein AM, Badawoud MH, Mustafa, HN (2013) The effects of diethylstilbestrol administration on rat kidney Ultrastructural study. Saudi Med J, 34: 1114–1124.
• 27. Khan M, Mostofa M, Jahan M, Sayed M, Hossain M (2009) Effect of garlic and vitamin B-complex in lead acetate induced toxicities in mice. Bangl J Vet Med, 6: 203–210.
• 28. Kilikdar D, Mukherjee D, Mitra E, Ghosh AK, Basu A, Chandra AM, Bandyoapdhyay D (2011) Protective effect of aqueous garlic extract against lead-induced hepatic injury in rats. Indian J Exp Biol, 49: 498–510.
• 29. Li Y, Xu Sy (2007) Preparation of Garlic Powder with High Allicin Content by using combined microwave–vacuum and vacuum drying as well as microencapsulation. J Food Eng, 83: 76–83.
• 30. Malecka A, Jarmuszkiewicz W, Tomaszewska B (2001) Antioxidative defense to lead stress in subcellular compartments of pea root cells. Acta Biochim Pol, 48: 687–698.
• 31. Mousa AM, Al-Fadhli AS, Rao MS, Kilarkaje N (2015) Gestational lead exposure induces developmental abnormalities and up-regulates apoptosis of fetal cerebellar cells in rats. Drug Chem Toxicol, 38: 73–83.
• 32. Mustafa HN (2012) Effect of acrylamide on testis of albino rats Ultrastructure and DNA cytometry study. Saudi Med J, 33: 722–731.
• 33. Mustafa HN, El Awdan SA, Hegazy GA (2013) Protective role of antioxidants on thioacetamide-induced acute hepatic encephalopathy: biochemical and ultrastructural study. Tissue Cell, 45: 350–362.
• 34. Nagata M (2005) Inflammatory cells and oxygen radicals. Curr Drug Targets Inflamm Allergy, 4: 503–504.
• 35. Palan PR, Shaban DW, Martino T, Mikhail MS (2004) Lipid-soluble antioxidants and pregnancy: maternal serum levels of coenzyme Q10, alpha-tocopherol and gamma-tocopherol in preeclampsia and normal pregnancy. Gynecol Obstet Invest, 58: 8–13.
• 36. Pourjafar M, Aghbolaghi P, Shakhse-Niaie M (2007) Effect of garlic along with lead acetate administration on lead burden of some tissues in mice. Pak J Biol Sci, 10: 2772–2774.
• 37. Ronchetti R, Hazel P, Schoeters G, Hanke W, Rennezova Z, Barreto M, Villa MP (2006) Lead neurotoxicity in children: Is prenatal exposure more important than postnatal exposure? Acta Paediatr Suppl, 95: 45–49.
• 38. Roshan VD, Assali M, Moghaddam AH, Hosseinzadeh M, Myers J (2011) Exercise training and antioxidants: effects on rat heart tissue exposed to lead acetate. Int J Toxicol, 30: 190–196.
• 39. Schmahmann JD (2004) Disorders of the cerebellum: ataxia, dysmetria of thought, and the cerebellar cognitive affective syndrome. J Neuropsychiatry Clin Neurosci, 16: 367–378.
• 40. Sepúlveda A, Schluep M, Renaud FG, Streicher M, Kuehr R, Hagelüken C, Gerecke AC (2010) A review of the environmental fate and effects of hazardous substances released from electrical and electronic equipments during recycling: examples from China and India. Environ Impact Asses Rev, 30: 28–41.
• 41. Sharma V, Sharma A, Kansal L (2010) The effect of oral administration of Allium sativum extracts on lead nitrate induced toxicity in male mice. Food Chem Toxicol, 48: 928–936.
• 42. Shrivastava S (2012) Amelioration of aluminium induced toxicity by Allium sativum. Sci Res Essays, 8: 168–177.
• 43. Sobaniec-Lotowska ME (2003) Ultrastructure of astrocytes in the cortex of the hippocampal gyrus and in the neocortex of the temporal lobe in experimental valproate encephalopathy and after valproate withdrawal. Int J Exp Pathol, 84: 115–125.
• 44. Sofroniew MV (2014) Multiple roles for astrocytes as effectors of cytokines and inflammatory mediators. Neuroscientist, 20: 160–172.
• 45. Suvarna KS, Suvarna SK, Layton C, Bancroft JD (2012) Bancroft’s Theory and Practice of Histological Techniques, Expert Consult: Online and Print, 7: Bancroft’s Theory and Practice of Histological Techniques: Elsevier Health Sciences.
• 46. Thach W (2014) Does the cerebellum initiate movement? Cerebellum, 13: 139–150.
• 47. Verhagen H, Buijsse B, Jansen E, Bueno-de-Mesquita B (2006) The state of antioxidant affairs. Nutr Today, 41: 244–250.
• 48. Victery W (1988) Evidence for effects of chronic lead exposure on blood pressure in experimental animals: an overview. Environ Health Perspect, 78: 71–76.
• 49. Villeda-Hernandez J, Mendez Armenta M, Barroso-Moguel R, Trejo-Solis M, Guevara J, Rios C (2006) Morphometric analysis of brain lesions in rat fetuses prenatally exposed to low-level lead acetate: correlation with lipid peroxidation. Histol Histopathol, 21: 609–617.
• 50. Wang Y, Wang S (2011) Effects of lead exposure on histological structure and antioxidant capacity in the cerebellum of 30-day-old mice. Neural Regen Res, 6: 1077–1081.
• 51. Wilhelmsson U, Li L, Pekna M, Berthold CH, Blom S, Eliasson C, Renner O, Bushong E, Ellisman M, Morgan TE, Pekny M (2004) Absence of glial fibrillary acidic protein and vimentin prevents hypertrophy of astrocytic processes and improves post-traumatic regeneration. J Neurosci, 24: 5016–5021.
• 52. Xu J, Yan H, Yang B, Tong L, Zou Y, Tian Y (2009) Effects of lead exposure on hippocampal metabotropic glutamate receptor subtype 3 and 7 in developmental rats. J Negat Results BioMed, 8: 5.
• 53. Yousef MI, Awad TI, Mohamed EH (2006) Deltamethrin-induced oxidative damage and biochemical alterations in rat and its attenuation by vitamin E. Toxicology, 227: 240–247.
• 54. Zhi-Wei Z, Ru-Lai Y, Gui-juan D, Zheng-yan Z (2005) Study on the neurotoxic effects of low-level lead exposure in rats. J Zhejiang Univ Sci B, 6: 686–692.

Identyfikatory

DOI

10.5603/FM.a2015.0076