Measurement of asymmetric dimethylarginine, nitric oxide levels and total antioxidant capacity in experimental diabetic rats

• Autorzy: Surmeli E. , Takellii H. , Kiral F.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study is to investigate the status of oxidative stress and nitric oxide related parameters in diabetic rats. In experimental animals (12 rats) diabetes was induced by intraperitoneal injection of a single 50 mg/kg dose of streptozotocin. At the end of the experiment, in the blood serum of the control and experimental animals ADMA, NO was measured by using ELISA test. Serum levels of GSH, MDA and ceruloplasmin as well as TAC were detected in all animals. Compared to the control animals serum MDA levels in diabetic rats were remarkably and significantly (P < 0.05) higher (26.38 ± 1.94 µmol/L and 42.23 ± 1.24 µmol/L, respectively). On the other hand, a statistically significant (P < 0.05) decrease from 4.39 ± 0.15 mg/dl to 4.00 ± 0.09 mg/dl was detected in serum GSH levels of the diabetic rats. Compared to the serum ceruloplasmin levels of healthy rats (35.05 ± 2.79 mg/dl), diabetic rats showed a significant decrease (P < 0.001) in their serum ceruloplasmin concentration (23.10 ± 1.65 mg/dl). Likewise, serum ADMA concentration of experimental animals (7.26 ± 0.86 ng/ml) was higher than that of controls (5.59 ± 0.75 ng/ml) but not significantly (P > 0.05). As to serum TAC, compared to the control rats a statistically significant (P < 0.05) decline was observed in diabetic rats. Serum TAC of diabetic rats reduced from 1.01 ± 0.06 mmol trolox equivalent/L to 0.87 ± 0.03 mmol trolox equivalent/L. Serum NO concentration of experimental animals (30.42 ± 2.48 µM/ml) was significantly (P < 0.05) less than that of controls (62.28 ± 10.74 µM/ml).

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2017
• Tom: 73
• Numer: 07
• Opis fizyczny: p.418-421,ref.

Twórcy

autor

Surmeli E.

• Department of Biochemistry, Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey

autor

Takellii H.

• Golhisar Vocational High School of health Service, Mehmet Akif Ersoy University, Burdur, Turkey

autor

Kiral F.

• Department of Biochemistry, Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey

Bibliografia

• Abou-Seif M. A., Youssef A. A.: Evaluation of some biochemical changes in diabetic patients. Clin. Chim. Acta 2004, 346, 161-170.
• Akkaya H., Çelik S.: Ratlarda diyabet öncesi ve sonrası oksidan-antioksidan durum, F.Ü. Sağ. Bil. Vet. Der. 2010, 24, 5-10.
• Baynes J. W., Thorpe S. R.: Role of oxidative stress in diabetic complications: A new perspective on an old paradigm. Diabetes 1999, 48, 1-9.
• Bedoya F. J., Solano F., Lucas M.: N-monomethyl-arginine and nicotinamide prevent streptozotocine-induced double strand DNA break formation in pancreatic islets. Experientia 1996, 52, 344-347.
• Beutler E., Dubon O., Kelly B. M.: Improved method for the determination of blood glutathione. J. Lab. Clın. Med. 1963, 61, 882-888.
• Byung P. Y.: Celluler defences agains damage from reactive species. Physıol. Rev. 1994, 74, 139-172.
• Dosoo D. K., Rana S. V., Offe-Amoyaw K., Tete-Donkor D., Maddy S. Q.: Total antioxidant status in non-insulin-dependent diabetes mellitus patients in Ghana. West Afric J. Med. 2001, 20, 184-186.
• Elabbady A. A., Gagnon C., Hassouna M. M.: Diabetes mellitus increase NO synthase in penises but not in majority of rats. Br. J. Urol. 1995, 76, 196-202.
• Elmalı E., Altan N., Bukan N.: Effect of sulphonylurea glibenclamide on liver and kidney antioxidant enzymes in streptozotocin-induced diabetic rats. Drugs R. D. 2004, 5, 203-208.
• Erel O.: A novel automated method to measure total antioxidant response against potent free radical reactions. Clin. Biochem. 2004, 37, 112-119.
• Ghiselli A., Serafini M., Natella F., Scaccini C.: Total antioxidant capacity as a tool to assess redox status: critical view and experimental data. Free Radic. Biol. Med. 2000, 29, 1106-1114.
• Gmieniczek A., Hopkala H., Wojtowicz Z., Nieradko M.: Differences in antioxidant status in skeletal muscle tissue in experimental diabetes. Clin. Chim. Acta 200, 314, 39-45.
• Gönül B., Demiröz A. S., Özer Ç.: The oxidant/antioxidant capacity of diabetic rat liver tissue treated with benfluorex. Faseb J. 2005, 19, A 1531.
• Haluzik M., Nedvidkova J.: The role of nitric oxide in the development of streptozotocine induced diebetes mellitus: experimental and clinical implications. Physiol. Res. 2000, 49, 37-42.
• Khandelwal R. L., Gupta D., Sulakhe P. V.: Decreased activity and impaired induction of nitric oxide synthase by lipopolysaccharides in streptozotocineinduced diabetic rats. Bioc. Bioph. Acta 2003, 1620, 259-266.
• Krzyzanowska K., Mittermayer F., Krugluger W., Schnack C., Hofer M., Wolzt M., Schernthaner G.: Asymmetric dimethylarginine is associated with macrovascular disease and total homocysteine in patients with type 2 diabetes. Atherosclerosis 2006, 189, 236-240.
• Lin K. Y., Ito A., Asagami T., Tsao P. S., Adimoolam S., Kimoto M., Tsuji M., Reaven G. M., Cooke J. P.: Impaired nitric oxide synthase pathway in diabetes mellitus: role of asymmetric dimethylarginine and dimethylarginine dimethylaminohydrolase. Circulation 2002, 106, 987-992.
• Mesa J., Salcedo D., Calle H., Delgado E., Novoa J., Hawkins F., Navarrete G. S., Parramon M., Acosta D.: Detection of ketonemia and its relationship with hyperglycemia in type I Diabetic patients. Diabetes Res. Clin. Pract. 2006, 72, 292-297.
• Mohan K., Das U. N.: Effect of L-arginine-nitric oxide system on the metabolism of essential fatty acids in chemical-induced diabetes mellitus. Prostaglandins Leukot. Essent. Fatty Acids 2000, 62, 35-46.
• Nukatsuka M., Yoshimura Y., Nishida M., Kawadw J.: Importance of the concentration of ATP in rat pancreatic beta cells in the mechanism of streptozotocine-induced cytotoxicity. J. Endocrinol. 1990, 127, 161-165.
• Opara E. C., Abdel-Rahman E., Soliman S., Kamel W. A., Souka S., Lowe J. E., Abdel-Aleem S.: Depletion of total antioxidant capacity in type 2 diabetes. Metabolism 1999, 48, 1414-1417.
• Paiva H., Lehtimaki T., Laakso J., Ruokonen I., Rantalaiho V., Wirta O., Pasternack A., Laaksonen R.: Plasma concentrations of asymmetric-dimethylarginine in type 2 diabetes associate with glycemic control and glomerular filtration rate but not with risk factors of vasculopathy. Metabolism 2003, 52, 303-307.
• Serafini M., Del Rio D.: Understanding the association between dietary antioxidants, redox status and disease: is the total antioxidant capacity the right tool? Redox Rep. 2004, 9, 145-152.
• Shanmugam K. R., Mallikarjuna K., Kesireddy M., Reddy K. S.: Neuroprotective effect of ginger on anti-oxidant enzymes in streptozotocin-induced diabetic rats. Food Chem. Toxicol. 2011, 41, 893-897.
• Shin M.-J., Park E., Lee J. H., Chung N.: Relationship between insulin resistance and lipid peroxidation and antioxidant vitamins in hypercholesterolemic patients. Ann. Nutr. Metab. 2006, 50, 115-120.
• Subramanian V., Leelavinothan P., Ganesan S.: Effect of Phaseolus vulgaris on Circulatory Antioxidants and Lipids in Rats with Streptozotocin Induced Diabetes. J. Med. Food. 2002, 5, 97-103.
• Sunderman F. W., Nomoto S.: Measurement of human serum ceruloplasmin by its p-phenylene-diamine oxidase activity. Clin. Chem. 1970, 16, 903-910.
• Szkudelski T.: The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol. Res. 2001, 50, 536-546.
• Vallance P., Leiper J.: Cardiovascular Biology of the Asymmetric Dimethylarginine: Dimethylarginine Dimethylaminohydrolase Pathway. Arterioscler Thromb. Vasc. Biol. 2004, 24, 1023-1030.
• Wiernsperger N. F.: Oxidative stress as a therapeutic target in diabetes: revisiting the controversy. Diabetes Metab. 2003, 29, 1-14.
• Yanwen W., Tony C., Benjamin P., Cécile B., Ling Q.: Hypoglycemic and insulin-sensitizing effects of berberine in high-fat diet- and streptozotocininduced diabetic rats. Metabolism 2011, 60, 298-305.
• Yılmaz H. R., Uz E., Yücel N., Altuntaş I., Ozçelik N.: Protective effect of caffeic acid phenethyl ester (CAPE) on lipid peroxidation and antioxidant enzymes in diabetic rat liver. J. Biochem. Mol. Toxicol. 2004, 18, 234-238.
• Yoshoiko T., Kawada K., Shimada T.: Lipit peroxidation in maternal and cord blood and protective mechanism aganist actived-oxygen toxicity in the blood. Am. J. Obstet. Gynecol. 1979, 135, 372-376.

Identyfikatory

DOI

10.21521/mw.5741