Auxin signalling is involved in cadmium-induced glutathione-S-transferase activity in barley root

• Autorzy: Bocova B. , Huttova J. , Mistrik I. , Tamas L.
• Języki publikacji: EN

Abstrakty

EN

Transient exposure of barley roots to Cd, IAA or H2O2 for 30 min resulted in a significant root growth inhibition. Cd significantly increased the GST activity of roots 6 h after the end of short-term treatment. This increase was more relevant in root segment containing differentiation zone than in root segment just immediately behind the root apex. In contrast to Cd treatment, the shortterm exposure of barley roots to IAA resulted in a significant increase of GST activity along the whole root tip and this increase was detectable already 3 h after the treatment with 10 μM IAA. Similarly to IAA, exogenously applied 10 mM H2O2 for 30 min caused significant increase of GST activity along the whole root tip 6 h after the treatment. This increase was already detectable 3 h after the exposure, but only in the differentiation zone of root tip. Auxin influx or signalling inhibitor considerable decreased the Cd- or IAA-induced GST activity in barley root tips. The strong activation of GST even after a brief exposure of barley roots to Cd support the crucial role of GST in the Cd-induced stress response in which presumably IAA and H2O2 play an important signalling role including the activation of GST.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 09
• Opis fizyczny: p.2685-2690,fig.,ref.

Twórcy

autor

Bocova B.

• Institute of Botany, Slovak Academy of Sciences, Du´bravska´ cesta 9, 84523 Bratislava, Slovak Republic

autor

Huttova J.

• Institute of Botany, Slovak Academy of Sciences, Du´bravska´ cesta 9, 84523 Bratislava, Slovak Republic

autor

Mistrik I.

• Institute of Botany, Slovak Academy of Sciences, Du´bravska´ cesta 9, 84523 Bratislava, Slovak Republic

autor

Tamas L.

• Institute of Botany, Slovak Academy of Sciences, Du´bravska´ cesta 9, 84523 Bratislava, Slovak Republic

Bibliografia

• Anjum NA, Ahmad I, Mohmood I, Pacheco M, Duarte AC, Pereira E, Umar S, Ahmad A, Khan NA, Iqbal M, Prasad MNV (2012) Modulation of glutathione and its related enzymes in plants’ responses to toxic metals and metalloids—a review. Env Exp Bot 75:307–324
• Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
• Chen W, Singh KB (1999) The auxin, hydrogen peroxide and salicylic acid induced expression of the Arabidopsis GST6 promoter is mediated in part by an ocs element. Plant J 19:667–677
• Chen F, Wu F, Dong J, Vincze E, Zhang G, Wang F, Huang Y, Wei K (2007) Cadmium translocation and accumulation in developing barley grains. Planta 227:223–232
• Coleman JOD, Blake-Kalff MMA, Davies TGE (1997) Detoxification of xenobiotics by plants: chemical modification and vacuolar compartmentation. Trends Plant Sci 2:144–151
• Cuypers A, Plusquin M, Remans T, Jozefczak M, Keunen E, Gielen H, Opdenakker K, Nair AR, Munters E, Artois TJ, Nawrot T, Vangronsveld J, Smeets K (2010) Cadmium stress: an oxidative challenge. Biometals 23:927–940
• DalCorso G, Farinati S, Maistri S, Furini A (2008) How plants cope with cadmium: staking all on metabolism and gene expression. J Integr Plant Biol 50:1268–1280
• Dixit V, Pandey V, Shyam R (2001) Differential antioxidative responses to cadmium in roots and leaves of pea (Pisum sativum L. cv. Azad). J Exp Bot 52:1101–1109
• Dixon DP, Cummins I, Cole DJ, Edwards R (1998) Glutathionemediated detoxification systems in plants. Curr Opin Plant Biol 1:258–266
• Droog FNJ, Hooykaas PJJ, Libbenga KR, van der Zaal EJ (1993) Proteins encoded by an auxin-regulated gene family of tobacco share limited but significant homology with glutathione S-transferases and one member indeed shows in vitro GST activity. Plant Mol Biol 21:965–972
• Edwards R, Dixon DP, Walbot V (2000) Plant glutathione S-transferase: enzymes with multiple functions in sickness and in health. Trends Plant Sci 5:193–198
• Elobeid M, Göbel C, Feussner I, Polle A (2012) Cadmium interferes with auxin physiology and lignification in poplar. J Exp Bot 63:1413–1421
• Ezaki B, Suzuki M, Motoda H, Kawamura M, Nakashima S, Matsumoto H (2004) Mechanism of gene expression of Arabidopsis glutathione S-transferase, AtGST1, and AtGST11 in response to aluminum stress. Plant Physiol 134:1672–1682
• Gong H, Jiao Y, Hu WW, Pua EC (2005) Expression of glutathione-S-transferase and its role in plant growth and development in vivo and shoot morphogenesis in vitro. Plant Mol Biol 57:53–66
• Grossmann K, Kwiatkowski J, Tresch S (2001) Auxin herbicides induce H2O2 overproduction and tissue damage in cleavers (Galium aparine L.). J Exp Bot 52:1811–1816
• Iannelli MA, Pietrini F, Fiore L, Petrilli L, Massacci A (2002) Antioxidant response to cadmium in Phragmites australis plants. Plant Physiol Biochem 40:977–982
• Joo JH, Bae YS, Lee JS (2001) Role of auxin-induced reactive oxygen species in root gravitropism. Plant Physiol 126:1055–1060
• Koreňovská M, Poláčeková O (2000) Trace elements content in virgin sunflower oil production. Czech J Food Sci 18:61–65
• Kunieda T, Fujiwara T, Amano T, Shioi Y (2005) Molecular cloning and characterization of a senescence-induced tau-class glutathione S-transferase from barley leaves. Plant Cell Physiol 46:1540–1548
• Marrs KA (1996) The functions and regulation of glutathione S-transferases in plants. Annu Rev Plant Physiol Plant Mol Biol 47:127–158
• Minglin L, Yuxiu Z, Tuanyao C (2005) Identification of genes up-regulated in response to Cd exposure in Brassica juncea L. Gene 363:151–158
• Moons A (2003) Osgstu3 and osgstu4, encoding tau class glutathione S-transferases, are heavy metal- and hypoxic stress-induced and differentially salt stress-responsive in rice roots. FEBS Lett 553:427–432
• Oono Y, Ooura C, Rahman A, Aspuria ET, Hayashi K, Tanaka A, Uchimiya H (2003) p-Chlorophenoxyisobutyric acid impairs auxin response in Arabidopsis root. Plant Physiol 133:1135–1147
• Ortega-Villasante C, Rellán-Álvarez R, Del Campo FF, Carpena-Ruiz RO, Hernández LE (2005) Cellular damage induced by cadmium and mercury in Medicago sativa. J Exp Bot 56:2239–2251
• Pan J, Plant JA, Voulvoulis N, Oates CJ, Ihlenfeld C (2010) Cadmium levels in Europe: implications for human health. Environ Geochem Health 32:1–12
• Polidoros AN, Scandalios JG (1999) Role of hydrogen peroxide and different classes of antioxidants in the regulation of catalase and glutathione S-transferase gene expression in maize (Zea mays L.). Physiol Plant 106:112–120
• Roth U, von Roepenack-Lahaye E, Clemens S (2006) Proteome changes in Arabidopsis thaliana roots upon exposure to Cd2+. J Exp Bot 57:4003–4013
• Roxas VP, Lodhi SA, Garrett DK, Mahan JR, Allen RD (2000) Stress tolerance in transgenic tobacco seedlings that overexpress glutathione S-transferase/glutathione peroxidase. Plant Cell Physiol 41:1229–1234
• Sappl PG, Carroll AJ, Clifton R, Lister R, Whelan J, Millar AH, Singh KB (2009) The Arabidopsis glutathione transferase gene family displays complex stress regulation and co-silencing multiple genes results in altered metabolic sensitivity to oxidative stress. Plant J 58:53–68
• Seppanen MM, Cardi T, Hyökki MB, Pehu E (2000) Characterization and expression of cold-induced glutathione S-transferase in freezing tolerant Solanum commersonii, sensitive S. tuberosum and their interspecific somatic hybrids. Plant Sci 153:125–133
• Sharma SS, Dietz K-J (2009) The relationship between metal toxicity and cellular redox imbalance. Trends Plant Sci 14:43–50
• Smith AP, Nourizadeh SD, Peer WA, Xu J, Bandyopadhyay A, Murphy AS, Goldsbrough PB (2003) Arabidopsis AtGSTF2 is regulated by ethylene and auxin, and encodes a glutathione S-transferase that interacts with flavonoids. Plant J 36:433–442
• Smith AP, DeRidder BP, Guo WJ, Seeley EH, Regnier FE, Goldsbrough PB (2004) Proteomic analysis of Arabidopsis glutathione S-transferases from benoxacor- and copper-treated seedlings. J Biol Chem 279:26098–26104
• Takahashi Y, Nagata T (1992) parB: an auxin-regulated gene encoding glutathione S-transferase. Proc Natl Acad Sci 89:56–59
• Tamás L, Bočová B, Huttová J, Liptáková L , Mistrik I, Valentovičová K, Zelinová V (2012) Impact of the auxin signaling inhibitor p-chlorophenoxyisobutyric acid on short-term Cd-induced hydrogen peroxide production and growth response in barley root tip. J Plant Physiol 169:1375–1381
• Ulmasov T, Ohmiya A, Hagen G, Guilfoyle T (1995) The soybean GH2/4 gene that encodes a glutathione S-transferase has a promoter that is activated by a wide range of chemical-agents. Plant Physiol 108:919–927
• Wang Y, Qian Y, Hu H, Xu Y, Zhang H (2011) Comparative proteomic analysis of Cd-responsive proteins in wheat roots. Acta Physiol Plant 33:349–357
• Xu F, Lagudah ES, Moose SP, Riechers DE (2002) Tandemly duplicated safener-induced glutathione S-transferase genes from Triticum tauschii contribute to genome- and organ-specific expression in hexaploid wheat. Plant Physiol 130:362–373

Uwagi

rekord w opracowaniu