How photothrombotic stroke affects extracellular matrix?

• Autorzy: Nowicka D. , Karetko M. , Aleksy M. , Skangiel-Kramska J.
• Języki publikacji: EN

Abstrakty

EN

Perineuronal net (PN), a lattice-like structure surrounding certain neurons, represents a special form of extracellular matrix of the brain. The role of this structure remains elusive. It has been suggested to regulate neuronal plasticity, accumulate trophic molecules and protect neurons from damaging factors. Here we studied whether PN-enwrapped neurons are protected against ischemic injury. To this aim we performed cortical photothrombotic stroke in rats and investigated PNs and their co-localization with Fluoro Jade C (FJ), a marker of rapid cell death. We found that in the ischemic core 4 h after the stroke still PNs were observable, none of them around FJ-positive neurons. No PNs were detected after 24 h. In the peri-infarct area, a huge loss of PNs was observed 24 h after stroke, followed by a partial restoration 30 days post-infarct. Similar effect was found in remote cortical areas, except that in this case the restoration was almost complete already 7 days after stroke. No FJ staining was observed outside ischemic core. These results suggest that in areas that are not directly damaged, ischemic insult disrupts PNs but the neurons survive this disruption. Supported by MNiSW Grant N3030300832/0474.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 3
• Opis fizyczny: p.286-287

Twórcy

autor

Nowicka D.

• Department of Molecular and Cellular Neuroscience, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Karetko M.

• Department of Molecular and Cellular Neuroscience, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Aleksy M.

• Department of Molecular and Cellular Neuroscience, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Skangiel-Kramska J.

• Department of Molecular and Cellular Neuroscience, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland