Microbial community dynamics in field-scale biopile bioremediation

• Autorzy: Tanase A.M. , Chiciudean I. , Mereuta I. , Ionescu R. , Cornea C.P. , Vassu T. , Stoica I.
• Języki publikacji: EN

Abstrakty

EN

Understanding the microbial communities responses to biopile procedures is an essential step in the field of microbial ecology and in bioremediation applications. In order to estimate metabolic dynamics at a microbial community level, we used Biolog Ecoplates. Assays showed that the bacterial communities from all samples were capable of utilizing a great variety from the 31 carbon substrates. Shannon’s diversity index values were between 2.711±0.303 in August and 3.262±0.062 in April, recording a general variation of 0.5 that is similar to other studies. Statistical analysis showed a good correlation coeficient for 2-hydroxybenzoic acid, a compound that is metabolized through the nafhthalene degradation pathway. Results showed a metabolic shift of the microbial community determined by temperature and the decrease of aromatic hydrocarbon concentrations. The shift was observed in the dynamics of MPN from 9x10⁶ to 1x10⁹ cell/g of soil. Even if the initial total petroleum hydrocarbon concentration from the biopile matrix was much higher than in other studies (10.25 g/kg), at the end of the evaluation the pollutant content of the biopile matrix had a removal rate between 92-98% for monoaromatic hydrocarbons and 57-75% for poliaromatic hydrocarbons.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2017
• Tom: 26
• Numer: 1
• Opis fizyczny: p.331-336,fig.

Twórcy

autor

Tanase A.M.

• Molecular Biology Laboratory, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Marasti Blvd, District 1, 011464, Bucharest, Romania
• 2 Department of Genetics, University of Bucharest, 1-3 Portocalelor Aly, Districs 6, 060101, Bucharest, Romania

autor

Chiciudean I.

• Department of Genetics, University of Bucharest, 1-3 Portocalelor Aly, Districs 6, 060101, Bucharest, Romania

autor

Mereuta I.

• Department of Genetics, University of Bucharest, 1-3 Portocalelor Aly, Districs 6, 060101, Bucharest, Romania

autor

Ionescu R.

• Department of Genetics, University of Bucharest, 1-3 Portocalelor Aly, Districs 6, 060101, Bucharest, Romania

autor

Cornea C.P.

• Molecular Biology Laboratory, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Marasti Blvd, District 1, 011464, Bucharest, Romania

autor

Vassu T.

• Molecular Biology Laboratory, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Marasti Blvd, District 1, 011464, Bucharest, Romania

autor

Stoica I.

• Department of Genetics, University of Bucharest, 1-3 Portocalelor Aly, Districs 6, 060101, Bucharest, Roman

Bibliografia

• 1. BALDAN E., BASAGLIA M., FONTANA F., SHAPLEIGH J. P., CASELLA S. Development, assessment and evaluation of a biopile for hydrocarbons soil remediation International Biodeterioration & Biodegradation. 98, 66, 2015.
• 2. ALISI C., MUSELLA R., TASSO F., UBALDI C., MANZO S., CREMISINI C., ROSA SPROCATI A. Bioremediation of diesel oil in a co-contaminated soil by bioaugmentation with a microbial formula tailored with native strains selected for heavy metals resistance, Science of the Total Environment. 407, 3024, 2009.
• 3. TĂNASE A-M., MEREUTA I., CHICIUDEAN I., IONESCU R., CORNEA C.P., VASSU T., STOICA I Metabolic and molecular dynamics of microbial communities from kerosene polluted microcosm. Journal of Biotechnology. 208, Supplement, S64, 2015.
• 4. AICHNER B., BUSSIAN B. M., LEHNIK-HABRINK P., HEIN S. Regionalized concentrations and fingerprints of polycyclic aromatic hydrocarbons (PAHs) in German forest soils Environmental Pollution. 203, 31, 2015.
• 5. CHEN J., XIE H., ZHUANG X., ZHUANG G., BAI Z., ZHANG H. Substrate-induced changes in microbial community-level physiological profiles and their application to discriminate soil microbial Communities. Journal of Environmental Sciences. 20, 725, 2008.
• 6. AL-MUTAIRI N.Z. Variable distributional characteristics of substrate utilization patterns in activated sludge plants in Kuwait, Bioresource Technology. 100, 1524, 2009.
• 7. TIQUIA S.M. Metabolic diversity of the heterotrophic microorganisms and potential link to pollution of the Rouge River. Environ. Pollut. 158, 1435, 2009.
• 8. TĂNASE A.M., MEREUTA I., CHICIUDEAN I., IONESCU R., MILEA L., CORNEA C.P., VASSU T., STOICA Comparison of total DNA extraction methods for microbial community from polluted soil I. Agriculture and Agricultural Science Procedia. 6, 616, 2015.
• 9. BRADLEY R.L., SHIPLEY B., BEAULIEU C. Refining numerical approaches for analyzing soil microbial community catabolic profiles based on carbon source utilization patterns. Soil Biology & Biochemistry. 38, 629, 2006.
• 10. STEFANOWICZ A. The Biolog Plates Technique as a Tool in Ecological Studies of Microbial Communities A. Polish J. of Environ. Stud. 15 (5), 669, 2006.
• 11. CHEN L., CAI Q., XU S., LIU X., CHEN S. Distributuion characteristics, pollution assessment and source identification of heavy metals in sediments of Wetland Lakes. Pol.J. Environ.Stud. 24, 1525, 2015.
• 12. WANG X., WANG Q., WANG S., LI F., GUO G. Effect of biostimulation on community level physiological profiles of microorganisms in field-scale biopiles composed of aged oil sludge Bioresource Technology. 111, 308, 2012.
• 13. SANDERS J.G., SPLIID H., ALBRECHTSEN H.J., Microbial Community-Level Physiological Profiles (CLPP) and herbicide mineralization potential in groundwater affected by agricultural land use. Journal of Contaminant Hydrology 140, 45, 2012.
• 14. Roumanian Stadard ISO SR 7877-2:1995 Determination of total petroleum hydrocarbons from suspentions Part 2: Method using spectrofotmetry.
• 15. Roumanian Stadard ISO 11423-2:1997 Water quality - Determination of benzene and some derivatives - Part 2: Method using extraction and gas chromatography
• 16. Roumanian Stadard SR-ISO 13877:1998. Soil quality - Determination of polynuclear aromatic hydrocarbons - Method using high -performance liquid chromatography
• 17. SR EN ISO 17294-2:2003 Water quality - Application of inductively coupled plasma mass spectrometry (ICP-MS) - Part 2: Determination of 62 elements.
• 18. https://www.microb3.eu/sites/default/files/deliverables/MB3_D5_9_PU.pdf.
• 19. CARDINALI-REZENDE J., MORAES A.M.M., COLTURATO L.F.D.B., CARNEIRO E.V., MARRIEL I.E. CHARTONE-SOUZA E., NASCIMENTO A.M. A. Phylogenetic and physiological characterization of organic waste-degrading bacterial communities. World J Microbiol Biotechnol. 27, 245, 2011.
• 20. LEE E.H., KIM J., KIM J.Y., KOO S.Y., LEE S.D., KO K.S., KO D.C., YUM B.W., CHO K.S. Comparison of microbial communities in petroleum-contaminated groundwater using genetic and metabolic profiles at Kyonggi-Do, South Korea. Environ Earth Sci 60, 371, 2010.
• 21. FANG H., YU Y.,CHU X., WANG X., YANG X., YU J. Degradation of chlorpyrifos in laboratory soil and its impact on soil microbial functional diversity. Journal of Environmental Sciences. 21, 380, 2009.
• 22. STEFANOWICZ A. The Biolog Plates Technique as a Tool in Ecological Studies of Microbial Communities A. Polish J. of Environ. Stud. 15 (5), 669, 2006.
• 23. LOPES A.R., FARIA C., PRIETO-FERNÁNDEZ A., TRASAR-CEPEDA C., MANAIA C.M., NUNES OLGA C. Comparative study of the microbial diversity of bulk paddy soil of two rice fields subjected to organic and conventional farming. Soil Biology & Biochemistry. 43, 115, 2011.
• 24. WALLENIUS K, LAPPI K, MIKKONEN A, WICKSTRÖM A, VAALAMA A, LEHTINEN T, SUOMINEN L. Simplified MPN method for enumeration of soil naphthalene degraders using gaseous substrate. Biodegradation. 23, 47, 2012.
• 25. GRAHAM M.H., HAYNES R.J. Catabolic diversity of soil microbial communities under sugarcane and other land uses estimated by Biolog and substrate-induced respiration methods. Applied Soil Ecology. 29, 155, 2005.
• 26. TENG Y., LUO Y., SUNM., LIU Z., LI Z., CHRISTIE P., Effect of bioaugmentation by Paracoccus sp. strain HPD-2 on the soil microbial community and removal of polycyclic aromatic hydrocarbons from an aged contaminated soil. Bioresource Technology. 101, 3437, 2010.
• 27. MELNYK A., DETTLAFF A., KUKLIŃSKA K., NAMIEŚNIK J., WOLSKA L. Concentration and sources of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) in surface soil near a municipal solid waste (MSW) landfill. Science of the Total Environment. 530, 18, 2015.

Identyfikatory

DOI

10.15244/pjoes/64644