Characterization and gene mapping of a brittle culm mutant of diploid wheat (Triticum monococcum L.) with irregular xylem vessels development

• Autorzy: Ansari M.J. , Al-Ghamdi A. , Usmani S. , Kumar R. , Nuru A. , Singh K. , Dhaliwal H. S.
• Języki publikacji: EN

Abstrakty

EN

Diploid wheat, Triticum monococcum L. (einkorn) is an ideal plant material for wheat functional genomics. Brittle culm mutant was identified by screening of the ethyl methane sulphonate-treated M2 progenies of a T. monococcum accession pau14087 by banding the plant parts manually. The brittle culm mutant with drooping leaves, early flowering, reduced tiller numbers and susceptible to lodging had also exhibited brittleness in all plant parts than the wild-type parents. Comprehensive mechanical strength, histological, biochemical, scanning electron microscopy, and Fourier transform infrared spectroscopy analyses of brittle culm mutant supplemented and complemented the findings that the mutant had defective cellulose biosynthesis pathway and deposition of cell wall materials on secondary cell wall of mechanical tissues. Microscopic studies demonstrated that the decrease in cellulose contents resulted in the irregular cell wall organization in xylem vessels of leaf vascular bundles. To map the brc5 mutant, mapping populations were developed by crossing the brittle culm mutant with wild Triticum boeoticum acc. pau5088, having non-brittle characters. The brittle culm mutation was mapped between SSR markers, Xcfd39 and Xgwm126 on 5AmL chromosome of T. monococcum, with genetic distances of 2.6 and 4.8 cM, respectively. The brc5 mutant mapped on 5AmL, being distinct from a previously mapped brittle culm mutant in wheat, has been designated as brc5. The work on fine mapping and map-based cloning of brc5 gene regulating synthesis and deposition of cellulose on the secondary cell wall is in progress.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 08
• Opis fizyczny: p.2407-2419,fig.,ref.

Twórcy

autor

Ansari M.J.

• Department of Plant Protection, College of Food and Agriculture Sciences, King Saud University, PO Box 2460, Riyadh 11451, Kingdom of Saudi Arabia
• Department of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand 247667, India

autor

Al-Ghamdi A.

• Department of Plant Protection, College of Food and Agriculture Sciences, King Saud University, PO Box 2460, Riyadh 11451, Kingdom of Saudi Arabia

autor

Usmani S.

• Department of Biotechnology, ICFAI University, Dehradun 248197, India

autor

Kumar R.

• D.K.M. College for Women, Thiruvalluvar University, Vellore, Tamilnadu 632004, India

autor

Nuru A.

• Department of Plant Protection, College of Food and Agriculture Sciences, King Saud University, PO Box 2460, Riyadh 11451, Kingdom of Saudi Arabia

autor

Singh K.

• School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab 141004, India

autor

Dhaliwal H. S.

• Department of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand 247667, India
• Akal School of Biotechnology, Eternal University, Baru Sahib, Himachal Pradesh 173101, India

Bibliografia

• Ansari MJ, Kumar R, Singh K, Dhaliwal HS (2012) Characterization and molecular mapping of EMS-induced brittle culm mutants of diploid wheat (Triticum monococcum L.). Euphytica 186:165–176
• Ansari MJ, Al-Ghamdi A, Kumar R, Usmani S, Al-attal Y, Adgaba N, Singh K, Dhaliwal HS (2013) Characterization and gene mapping of a chlorophyll-deficient mutant clm1 of Triticum monococcum L. Biol Plant. doi:10.1007/s10535-013-0307-3
• Aohara T, Kotake T, Kaneko Y, Takatsuji H, Tsumuraya Y, Kawasaki S (2009) Rice BRITTLE CULM 5(BRITTLE NODE) is involved in secondary cell wall formation in the sclerenchyma tissue of nodes. Plant Cell Physiol 50:1886–1897
• Appenzeller L, Doblin M, Barreiro R, Wang HY, Niu XM, Kollipara K, Carrigan L, Tomes D, Chapman M, Dhugga KS (2004) Cellulose synthesis in maize: isolation and expression analysis of the cellulose synthase (CesA) gene family. Cellulose 11: 287–299
• Bourne Y, Henrissat B (2001) Glycoside hydrolases and glycosyltransferases: families and functional modules. Curr Opin Struct Biol 11:593–600
• Brown DM, Zeef LA, Ellis J, Goodacre R, Turner SR (2005) Identification of novel genes in Arabidopsis involved in secondary cell wall formation using expression profiling and reverse genetics. Plant Cell 17:2281–2295
• Brown D, Wightman R, Zhang Z, Gomez LD, Atanassov I, Bukowski JP, Tryfona T, McQueen-Mason SJ, Dupree P, Turner S (2011) Arabidopsis genes IRREGULAR XYLEM (IRX15) and IRX15L encode DUF579-containing proteins that are essential for normal xylan deposition in the secondary cell wall. Plant J 66(3): 401–413
• Carpita N, McCann M (2000) The cell wall. In: Buchanan BB, Gruissem W, Jones RL (eds) Biochemistry and molecular biology of plants. American Society of Plant Physiologists, Rockville, pp 52–108
• Carpita N, Tierney M, Campbell M (2001) Molecular biology of the plant cell wall: searching for the genes that define structure, architecture and dynamics. Plant Mol Biol 47:1–5
• Chen L, Huang L, Min D, Phillips A, Wang S, Madgwick PJ, Parry MA, Hu YG (2012) Development and characterization of a new TILLING population of common bread wheat (Triticum aestivum L.). PLoS One 7(7):e41570. doi:10.1371/journal.pone.0041570
• Chhuneja P, Kaur S, Garg T, Ghai M, Kaur S, Prashar M, Bains NS, Goel RK, Keller B, Dhaliwal HS, Singh K (2008) Mapping of adult plant stripe rust resistance genes in diploid A genome wheat species and their transfer to bread wheat. Theor Appl Genet 116:313–324
• Ching A, Dhugga KS, Appenzeller L, Meeley R, Bourett TM, Howard RJ, Rafalski A (2006) Brittle stalk2 encodes a putative glycosylphosphatidylinositol-anchored protein that affects mechanical strength of maize tissues by altering the composition and structure of secondary cell walls. Planta 224:1174–1184
• Cosgrove DJ (2000) Loosening of plant cell walls by expansions. Nature 407(6802):321–326
• Demura T, Tashiro G, Horiguchi G, Kishimoto N, Kubo M, Matsuoka N, Minami A, Nagata-Hiwatashi M, Nakamura K, Okamura Y, Sassa N, Suzuki S, Yazaki J, Kikuchi S, Fukuda H (2002) Visualization by comprehensive microarray analysis of gene expression programs during trans differentiation of mesophyll cells into xylem cells. Proc Natl Acad Sci 99:15794–15799
• Dhaliwal HS, Multani DS, Sharma SK, Singh M (1987) Induction of useful variability in T. monococcum L. Crop Improv 14(1):1–5
• Dubcovsky J, Luo MC, Zhang GY, Bainsteitter R, Desai A, Kilian A, Kleinhofs A, Dvorak J (1996) Genetic map of diploid wheat T. monococcum L. and its comparison with maps of H. vulgare L. Genetics 143:983–999
• Haldane JBS (1919) The combination of linkage values, and the calculation of distance between loci of linked factors. J Genet 8:299–309
• Harholt J, Jensen JK, Sorensen SO, Orfila C, Pauly M, Scheller HV (2006) ARABINAN DEFICIENT 1 is a putative arabinosyltransferase involved in biosynthesis of pectic arabinan in Arabidopsis. Plant Physiol 140:49–58
• Hirano K, Kotake T, Kamihara K, Tsuna K, Aohara T, Kaneko Y, Takatsuji H, Tsumuraya Y, Kawasaki S (2010) Rice BRITTLE CULM 3 (BC3) encodes a classical dynamin OsDRP2B essential for proper secondary cell wall synthesis. Planta 232:95–108
• Kokubo A, Kuraishi S, Sakurai N (1989) Culm strength of barley correlation among maximum bending stress, cell wall dimensions, and cellulose content. Plant Physiol 91:876–882
• Kokubo A, Sakurai N, Kuraishi S, Takabe K (1991) Culm brittleness of barley (Hordeum vulgare L.) mutants is caused by smaller number of cellulose molecules in cell wall. Plant Physiol 97:509–514
• Kosambi DD (1944) The estimation of map distance from recombination values. Ann Eugen 12:172–175
• Kubicka H, Kubicki B (1988) Characteristics of chemical composition of two forms of rye with brittle Stalk conditioned by Bs-1 or Bs- 2 genes. Hodowla Roslin Aklimatyzacja i Nasiennictwo 32:1–6
• Kubo M, Udagawa M, Nishikubo N, Horiguchi G, Yamaguchi M, Ito J, Mimura T, Fukuda H, Demura T (2005) Transcription switches for protoxylem and metaxylem vessel formation. Genes Dev 19:1855–1860
• Kuraparthy V, Sood S, Gill BS (2007) Genomic targeting and mapping of tiller inhibition gene (tin3) of wheat using ESTs and synteny with rice. Funct Integr Genomics 8:33–34
• Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
• Lee C, O’Neill MA, Tsumuraya Y, Darvill AG, Ye ZH (2007) The irregular xylem9 mutant is deficient in xylan xylosyltransferase activity. Plant Cell Physiol 48:1624–1634
• Li YH, Qian Q, Zhou YH, Yan MX, Sun L, Zhang M et al (2003) BRITTLE CULM1, which encodes a COBRA-like protein affects the mechanical properties of rice plant. Plant Cell 15: 2020–2031
• Li X, Yang Y, Yao J, Chen G, Li X, Zhang Q, Wu C (2009) FLEXIBLE CULM 1 encoding a cinnamyl-alcohol dehydrogenase controls culm mechanical strength in rice. Plant Mol Biol 69:685–697
• Lincoln SE, Daly MJ, Lander ES (1993) Constructing genetic maps with MAPMAKER/EXP version 3.0: a tutorial and reference manual. Whitehead Inst Biomed Res Tech Rpt, 3rd edn. Whitehead Institute for Biomedical Research, Cambridge, p 97
• Liu R, Yu H, Huang Y (2005) Structure and morphology of cellulose in wheat straw. Cellulose 12(1):25–34
• Liu S, Yeh CT, Tang HM, Nettleton D, Schnable PS (2012) Gene mapping via bulked segregant RNA-Seq (BSR-Seq). PLoS One 7(5):e36406. doi:10.1371/journal.pone.0036406
• Mou ZL, He YK, Dai Y, Liu XF, Li J (2000) Deficiency in fatty acid synthase leads to premature cell death and dramatic alteration in plant morphology. Plant Cell 12:405–417
• Nagao S, Takahashi M (1963) Trial construction of twelve linkage group in Japanese rice (genetical studies on rice plant, XXVII). J Fac Agric Hokkaido Univ 53:72–130
• Peña MJ, Zhong R, Zhou GK, Richardson EA, O’Neill MA, Darvill AG, York WS, Ye ZH (2007) Arabidopsis irregular xylem8 and irregular xylem9: implications for the complexity of glucuronoxylan biosynthesis. Plant Cell 19:549–563
• Persson S, Caffall KH, Freshour G, Hilley MT, Bauer S, Poindexter P, Hahn MG, Mohnen D, Somerville C (2007) The Arabidopsis irregular xylem8 mutant is deficient in glucuronoxylan and homogalacturonan, which are essential for secondary cell wall integrity. Plant Cell 19:237–255
• Sado PE, Tessier D, Vasseur M, Elmorjani K, Guillon F, Saulnier L (2009) Integrating genes and phenotype: a wheat–Arabidopsis–rice glycosyltransferase database for candidate gene analyses. Func Integr Genomics 9(1):43–58
• Saghai-Maroof MA, Soliman KM, Jorgensen AR, Allard RW (1984) Ribosomal DNA spacer length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci 81:8014–8018
• Singh K, Multani DS, Khush GS (1994) A new brittle culm mutant in rice. Rice Gen News 11:91–92
• Singh K, Ghai M, Garg M, Chhuneja P, Kaur P, Schnurbusch T, Keller B, Dhaliwal HS (2007) An integrated molecular linkage map of diploid wheat based on a Triticum boeoticum 9 T. monococcum RIL population. Theor Appl Genet 115:301–312
• Singh K, Chhuneja P, Singh I, Sharma SK, Garg T, Garg M, Keller B, Dhaliwal HS (2010) Molecular mapping of cereal cyst nematode resistance in T. monococcum L. and its transfer to genetics background of cultivated wheat. Euphytica 176:213–222
• Sood S, Kuraparthy V, Bai G, Gill BS (2009) The major threshability genes soft glume (sog) and tenacious glume (tg), of diploid and polyploid wheat, trace their origin to independent mutations at non-orthologous loci. Theor Appl Genet 119:341–351
• Taenzler B, Esposti RF, Vaccino P, Brandolini A, Effgen S, Heun M, Schafer-Pregl R, Borghi B, Salamini F (2002) Molecular linkage map of einkorn wheat: mapping of storage-protein and softglume genes and bread-making quality QTLs. Genet Res Camb 80:131–143
• Taylor NG, Scheible W-R, Cutler S, Somerville CR, Turner SR (1999) The irregular xylem3 locus of Arabidopsis encodes a cellulose synthase required for secondary cell wall synthesis. Plant Cell 11:769–780
• Taylor NG, Laurie S, Turner SR (2000) Multiple cellulose synthase catalytic subunits are required for cellulose synthesis in Arabidopsis. Plant Cell 12:2529–2540
• Taylor NG, Howells RM, Huttly AK, Vickers K, Turner SR (2003) Interactions among three distinct CesA proteins essential for cellulose synthesis. Proc Natl Acad Sci 100:1450–1455
• Turner SR, Somerville CR (1997) Collapsed xylem phenotype of Arabidopsis identifies mutants deficient in cellulose deposition in the secondary cell wall. Plant Cell 9:689–701
• Updegraff DM (1969) Semimicro determination of cellulose in biological materials. Anal Biochem 32:420–424
• Van Soest PJ, Robertson JB (1985) Analysis of forages and fibrous food, 74–75:80–82. In: A laboratory manual for animal science 613. Cornell University, Ithaca, New York
• Van Soest PJ, Robertson JB, Lewis BA (1991) Method for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 74:3583–3597
• Wang QS, Sang XC, Ling YH, Zhao FM, Yang ZL, Li Y, He GH (2009) Genetic analysis and molecular mapping of a novel gene for zebra mutation in rice (Oryza sativa L.). J Genet Genomics 36:679–684
• Wang D, Yuan S, Yin L, Zhao J, Guo B, Lan J, Li X (2012) A missense mutation in the transmembrane domain of CESA9 affects cell wall biosynthesis and plant growth in rice. Plant Sci 196:117–124
• Wicker T, Stein N, Albar L, Feuillet C, Schlagenhauf E, Keller B (2001) Analysis of a contiguous 211 kb sequence in diploid wheat (Triticum monococcum L.) reveals multiple mechanisms of genome evolution. Plant J 26:307–316
• Wu B, Zhang B, Dai Y, Zhang L, Shang-Guan K, Peng Y, Zhou Y, Zhu Z (2012) Brittle Culm15 encodes a membrane-associated chitinase-like protein required for cellulose biosynthesis in rice. Plant Physiol 159(4):1440–1452
• Xu J, Zhang Q-F, Zhang T, Zhang HY, Xu PZ, Wang XD, Wu XJ (2008) Phenotypic characterization, genetic analysis and genemapping for a brittle mutant in rice. J Integr Plant Biol 50: 319–328
• Yan L, Loukoianov A, Tranquilly G, Helguera M, Fahima T, Dubcovsky J (2003) Positional cloning of the wheat vernalization gene VRN1. Proc Natl Acad Sci 100:6263–6268
• Yan C, Yan S, Zeng X, Zhang Z, Gu M (2007) Fine mapping and isolation of Bc7(t), allelic to OsCesA4. J Genet Genom 34: 1019–1027
• Zhong R, Pena MJ, Zhou GK, Nairn CJ, Wood-Jones A, Richardson EA, Morrison WH, Darvill AG, York WS, Ye ZH (2005)
• Arabidopsis Fragile Fiber8, which encodes a putative glucuronyltransferase, is essential for normal secondary wall synthesis. Plant Cell 17:3390–3408
• Zuber M (1973) Evaluation of progress in selection for stalk quality. Corn Sorghum Res Conf Proc 28:110–122

Uwagi

rekord w opracowaniu