Cryopreservation of conditionally dormant orthodox seeds of Betula pendula

• Autorzy: Chmielarz P.
• Języki publikacji: EN

Abstrakty

EN

To evaluate the feasibility of long-term cryopreservation of Polish provenances of silver birch (Betula pendula), the sensitivity of conditionally dormant seeds to extreme desiccation and/or the ultra-low temperature of liquid nitrogen (LN; -196℃) was evaluated. The critical water content (WC) of desiccated seeds and the high-moisture freezing limit of seeds desiccated or moistened to various WCs and frozen for 24 h or for 2 years in LN was also determined. Germination tests revealed no critical WC for seeds [to 0.02 g H₂O g⁻¹ dry mass (g g⁻¹)]. Seeds tolerated freezing in LN within specific safe range of WC 0.02–0.23 g g⁻¹ (nuts). Seeds desiccated to the safe WC and stored in LN for 2 years had similar or higher germination as seeds stored at -3℃ for 2 years, depending on provenance. Therefore, long-term cryopreservation of B. pendula seeds in gene banks is feasible.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2010
• Tom: 32
• Numer: 3
• Opis fizyczny: p.591-596,fig.,ref.

Twórcy

autor

Chmielarz P.

• Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Ko´rnik, Poland

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