Melatonin prevents nicotine-induced hepatotoxicity by modulating apoptosis and histopathological changes in rats

• Autorzy: Sengul S.A. , İcen Taskın I. , Asır F. , Eraslan Sakar A. , Pektanc Sengul G.
• Języki publikacji: EN

Abstrakty

EN

Nicotine, the main toxic component of tobacco, directly or indirectly causes adverse effects on the liver metabolism. Melatonin, secreted by the pineal gland, has anti-apoptotic activity as well as antioxidant activity. The aim of this study was to reveal the antiapoptotic effects of melatonin in rats with experimentally induced chronic liver damage with nicotine. In this study, 32 male Wistar albino rats were divided into four groups: control, melatonin, nicotine and nicotine+melatonin. During the experiment, nicotine (1 mg/kg) and melatonin (10 mg/kg) were administered daily intraperitoneally for 56 days. At the end of the study, the liver tissues were taken for histopathological, immunohistochemical and molecular analysis. The administration of melatonin was determined to partially alleviate histopathological changes in the liver tissue induced by nicotine, such as hepatocyte degeneration, vascular dilatation and congestion, and leukocyte infiltration. It was observed that there was a significant decrease in Bax expression levels and a significant increase in Bcl-2 expression levels in the nicotine+melatonin group when compared to the injury group. On the other hand, it was determined that melatonin administration reduced the Bax/Bcl-2 ratio, which was significantly higher in the nicotine group compared to the other groups, to a level close to the control group. Additionally, as a result of immunohistochemical evaluation, it was observed that decreased Bax expression and increased Bcl-2 expression in hepatocytes in the nicotine+melatonin group were at a level close to the control group. Our results revealed that melatonin is a hepatoprotective and effective antioxidant by suppressing cell apoptosis and increasing the rate of healing after damage at both the immunohistochemical and molecular levels.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2024
• Tom: 27
• Numer: 4
• Opis fizyczny: p.589-597,fig.,ref.

Twórcy

autor

Sengul S.A.

• Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, Hatay, Turke

autor

İcen Taskın I.

• Department of Molecular Biology and Genetics, Faculty of Science and Art, Inonu University, Malatya, Turkey

autor

Asır F.

• Department of Histology and Embryology, Medical Faculty, Dicle University, Diyarbakır, Turkey

autor

Eraslan Sakar A.

• Department of Genetics, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, Hatay, Turkey

autor

Pektanc Sengul G.

• Technology and Research & Development Center (MARGEM), Hatay Mustafa Kemal University, Hatay, Turkey

Identyfikatory

DOI

10.24425/pjvs.2024.152949