Theta rhythm and local cell discharges recorded in posterior hypothalamic slices of the adult rat

• Autorzy: Caban B. , Klos-Wojtczak P. , Kowalczyk T. , Bocian R. , Kazmierska P. , Konopacki J.
• Języki publikacji: EN

Abstrakty

EN

BACKGROUND AND AIMS: Hippocampal formation (HPC) theta rhythm is one of the best examples of neural synchrony in mammalian brain. HPC theta field potentials in rats consists of high-amplitude, almost sinusoidal waves in 3–13 Hz frequency range. It is wellknown that the pathway of theta generation originates in the nucleus reticularis pontis oralis (RPO), then RPO projects to supramammillary nuclei (SuM), and finally through the medial septal area (MS) to HPC and other limbic structures. This tract is called the ascending brainstem-hippocampal synchronizing pathway. In vivo, HPC theta frequency is modulated at least partially by SuM which consists of neurons firing in the frequency of HPC theta. However, in our previous studies we have discovered for the first time that local theta activity can also be recorded in deafferented posterior hypothalamic preparations. The present in vitro study investigates theta-related neurons and their relation to local hypothalamic theta rhythm. METHODS: 56 in vitro electrophysiology experiments were performed using brain slices taken from 56 Wistar rats. Each slice was perfused with 75 µM carbachol (cholinergic agonist) to induce rhythmic activity. The relation of firing neurons to local field theta rhythm was investigated according to an earlier developed classification. RESULTS: This study resulted in recording 21 theta-related neurons and 35 neurons classified as non-related to theta. CONCLUSION: Neuronal activity recorded in the posterior hypothalamic area in vitro resembles well-documented patterns of thetarelated cell discharges in the hippocampal formation in vitro and in vivo. Supported by NCN grant 2013/11/B/NZ4/04873.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.S52

Twórcy

autor

Caban B.

• Neurobiology Department, University of Lodz, Lodz, Poland

autor

Klos-Wojtczak P.

• Neurobiology Department, University of Lodz, Lodz, Poland

autor

Kowalczyk T.

• Neurobiology Department, University of Lodz, Lodz, Poland

autor

Bocian R.

• Neurobiology Department, University of Lodz, Lodz, Poland

autor

Kazmierska P.

• Neurobiology Department, University of Lodz, Lodz, Poland

autor

Konopacki J.

• Neurobiology Department, University of Lodz, Lodz, Poland