PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2015 | 37 | 02 |
Tytuł artykułu

Suppression of α-l-arabinofuranosidase in the endosperm and atypical germination of lettuce seeds induced by sodium dichloroisocyanurate

Autorzy
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Endosperm cap (CAP) weakening and radicle (RAD) elongation growth are prerequisites for the completion of lettuce seed germination. Lettuce CAP and RAD cell walls contain a large amount of L-arabinofuranose, which may exist in the form of arabinan or L-arabinofuranose- rich polysaccharides. The aim of the present study was to investigate the relationship between a-L-arabinofuranosidase, an enzyme degrading L-arabinofuranose-rich polysaccharides, and CAP weakening and RAD elongation growth. It was shown that the enzyme activity and the transcript abundance of two lettuce α-L-arabinofuranosidase genes, LsARAf2 and LsARAf7, increased in the CAP and RAD prior to the completion of seed germination during imbibition in water. Sodium dichloroisocyanurate, a chemical inducing atypical germination (RAD elongation without endosperm rupture) in lettuce seeds, significantly decreased the enzyme activity and the transcript abundance of LsARAf7 and LsARAf2 in the CAP. It is concluded that the expression of α-L-arabinofuranosidase is necessary for CAP weakening and the suppression of this enzyme in the CAP is at least partly responsible for atypical germination in lettuce.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
37
Numer
02
Opis fizyczny
Article: 10 [7 p.], fig.,ref.
Twórcy
autor
  • Seed Science and Technology Laboratory, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
autor
  • Seed Science and Technology Laboratory, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
autor
  • Seed Science and Technology Laboratory, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
autor
  • Seed Science and Technology Laboratory, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
Bibliografia
  • Aspeborg H, Schrader J, Coutinho PM, Stam M, Kallas A, Djerbi S, Nilsson P, Denman S, Amini B, Sterky F, Master E, Sandberg G, Mellerowicz E, Sundberg B, Henrissat B, Teeri TT (2005)
  • Carbohydrate-active enzymes involved in the secondary cell wall biogenesis in hybrid aspen. Plant Physiol 137:983–997
  • Bewley JD (1997) Breaking down the walls-a role for endo-βmannanase in release from seed dormancy. Trend Plant Sci 2:464–469
  • Bewley JD, Leung DWM, Ouellette FB (1983) The cooperative role of endo-β-mannanase, β-manosidase and α-galactosidase in the mobilization of endosperm cell wall hemicelluloses of germinated lettuce seed. In: Nozzolillo C, Lea PJ, Loewus FA (ed) Mobilization of Reserves in Germination. Plenum Publishing Corporation, pp 137-152
  • Dutta S, Bradford KJ, Nevins DJ (1994) Cell-wall autohydrolysis in isolated endosperms of lettuce (Lactuca sativa L.). Plant Physiol 104:623–628
  • Ferré H, Broberg A, Duus JO, Thomsen KK (2000) A novel type of arabinoxylan arabinofuranohydrolase isolated from germinated barley: analysis of substrate preference and specificity by nanoprobe NMR. Eur J Biochem 267:6633–6641
  • Finch-Savage WE, Leubner-Metzger G (2006) Seed dormancy and the control of germination. New Phytol 171:501–523
  • Fulton LM, Cobbett CS (2003) Two a-L-arabinofuranosidase genes in Arabidopsis thaliana are differentially expressed during vegetative growth and flower development. J Exp Bot 54:2467–2477
  • Gomez LD, Steele-King CG, Jones L, Foster JM, Vuttipongchaikij S, McQueen-Mason SJ (2009) Arabinan metabolism during seed development and germination in Arabidopsis. Mol Plant 2:966–976
  • Halmer P, Bewley JD, Thorpe TA (1975) Enzyme to break down lettuce endosperm cell wall during gibberellin-and light-induced germination. Nature 258:716–718
  • Ikuma H, Thimann KV (1963) The role of the seed-coats in germination of photosensitive lettuce seeds. Plant Cell Physiol 4:169–185
  • Itai A, Ishihara K, Bewley JD (2003) Characterization of expression, and cloning, of β-D-xylosidase and α-L-arabinofuranosidase in developing and ripening tomato (Lycopersicon esculentum Mill.) fruit. J Exp Bot 54:2615–2622
  • Jones RL (1974) The structure of the lettuce endosperm. Planta 121:133–146
  • Lee RC, Burton RA, Hrmova M, Fincher GB (2001) Barley arabinoxylan arabinofuranohydrolases: purification, characterization and determination of primary structures from cDNA clones. Biochem J 356:181–189
  • Lee RC, Hrmova M, Burton RA, Lahnstein J, Fincher GB (2003) Bifunctional family 3 glycoside hydrolases from barley with α-L-arabinofuranosidase and β-D-xylosidase activity: characterization, primary structures, and COOH-terminal processing. J Biol Chem 278:5377–5387
  • Lee KJ, Dekkers BJ, Steinbrecher T, Walsh CT, Bacic A, Bentsink LON, Leubner-Metzger G, Knox JP (2012) Distinct cell wall architectures in seed endosperms in representatives of the Brassicaceae and Solanaceae. Plant Physiol 160:1551–1566
  • Minic Z, Rihouey C, Do CT, Lerouge P, Jouanin L (2004) Purification and characterization of enzymes exhibiting β-D-xylosidase activities in stem tissues of Arabidopsis. Plant Physiol 135:867–878
  • Minic Z, Do CT, Rihouey C, Morin H, Lerouge P, Jouanin L (2006) Purification, functional characterization, cloning, and identification of mutants of a seed-specific arabinan hydrolase in Arabidopsis. J Exp Bot 57:2339–2351
  • Nonogaki H, Morohashi Y (1999) Temporal and spatial pattern of the development of endo-β-mannanase activity in germinating and germinated lettuce seeds. J Exp Bot 50:1307–1313
  • Ouellette BFF, Bewley JD (1986) β-Mannoside mannohydrolase and the mobilization of the endosperm cell wall of lettuce seeds, cv. Gd Rapids Planta 169:333–338
  • Pavlisˇta AD, Haber AH (1970) Embryo expansion without protrusion in lettuce seeds. Plant Physiol 46:636–637
  • Pavlista AD, Valdovinos JG (1975) Carboxymethylcellulase activity prior to the onset of germination of lettuce seeds. Plant Physiol 56(2 Suppl):83
  • Ren YF, He JY, Wang XF (2007) Changes in activities of three enzymes degrading galactomannan during and following rice seed germination. Rice Sci 14:295–301
  • Saha BC (2000) α-L-Arabinofuranosidases: biochemistry, molecular biology and application in biotechnology. Biotechnol Adv 18:403–423
  • Skjøt M, Kauppinen S, Kofod LV, Fuglsang C, Pauly M, Dalbøge H, Andersen LN (2001) Functional cloning of an endo-arabinanase from Aspergillus aculeatus and its heterologous expression in A.oryzae and tobacco. Mol Genet Genom 265:913–921
  • Sliwinska E, Bassel GW, Bewley JD (2009) Germination of Arabidopsis thaliana seeds is not completed as a result of elongation of the radicle but of the adjacent transition zone and lower hypocotyl. J Exp Bot 60:3587–3594
  • Taiz L, Honigman WA (1976) Production of cell wall hydrolyzing enzymes by barley aleurone layers in response to gibberellic acid. Plant Physiol 58:380–386
  • Tateishi A, Mori H, Watari J, Nagashima K, Yamaki S, Inoue H (2005) Isolation, characterization, and cloning of α-L-arabinofuranosidase expressed during fruit ripening of Japanese pear. Plant Physiol 138:1653–1664
  • Wang A, Li J, Bewley JD (2004) Molecular cloning and characterization of an endo-β-mannanase gene expressed in the lettuce endosperm following radicle emergence. Seed Sci Res 14:267–276
  • Wang A, Wang X, Ren Y, Gong X, Bewley JD (2005) Endo-βmannanase and β-mannosidase activities in rice grains during and following germination, and the influence of gibberellin and abscisic acid. Seed Sci Res 15:219–227
  • Zhang Y, Chen B, Xu Z, Shi Z, Chen S, Huang X, Chen J, Wang X (2014) Involvement of reactive oxygen species in endosperm cap weakening and embryo elongation growth during lettuce seed germination. J Exp Bot 65:3189–3200
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-b4f788ec-a78b-4d81-a8bb-03de99786b27
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.