Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody

• Autorzy: Dai G. , Huang X. , Liu Q. , Li Y. , Zhang L. , Han K. , Yang J. , Liu Y. , Xue F. , Zhao D.
• Języki publikacji: EN

Abstrakty

EN

Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY36 within Cap protein. Sequence alignment indicated that 33QKVY36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2022
• Tom: 25
• Numer: 4
• Opis fizyczny: p.579-587,fig.,ref.

Twórcy

autor

Dai G.

• Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling Street, Nanjing City, Jiangsu Province, 210014, PR China
• College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Tongwei Road, Nanjing City, Jiangsu Province 210095, PR China
• Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing City, Jiangsu Province, 210014, PR China

autor

Huang X.

• Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling Street, Nanjing City, Jiangsu Province, 210014, PR China
• Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing City, Jiangsu Province, 210014, PR China

autor

Liu Q.

• Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling Street, Nanjing City, Jiangsu Province, 210014, PR China
• Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing City, Jiangsu Province, 210014, PR China

autor

Li Y.

• Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling Street, Nanjing City, Jiangsu Province, 210014, PR China
• Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing City, Jiangsu Province, 210014, PR China

autor

Zhang L.

• Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling Street, Nanjing City, Jiangsu Province, 210014, PR China
• Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing City, Jiangsu Province, 210014, PR China

autor

Han K.

• Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling Street, Nanjing City, Jiangsu Province, 210014, PR China
• Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing City, Jiangsu Province, 210014, PR China

autor

Yang J.

• Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling Street, Nanjing City, Jiangsu Province, 210014, PR China
• Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing City, Jiangsu Province, 210014, PR China

autor

Liu Y.

• Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling Street, Nanjing City, Jiangsu Province, 210014, PR China
• Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing City, Jiangsu Province, 210014, PR China

autor

Xue F.

• College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Tongwei Road, Nanjing City, Jiangsu Province 210095, PR China

autor

Zhao D.

• Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling Street, Nanjing City, Jiangsu Province, 210014, PR China
• College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Tongwei Road, Nanjing City, Jiangsu Province 210095, PR China
• Institute of Life Sciences, Jiangsu University, No. 301 Xuefu Road, Zhenjiang, Jiangsu Province, 212013, PR China
• Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing City, Jiangsu Province, 210014, PR China

Identyfikatory

DOI

10.24425/pjvs.2022.143541