Wybrane parametry walidacji zestawu AmpFl STR MiniFilerTM wykorzystywanego w genetyce sądowej

• Autorzy: Gryzinska M. , Szewczuk D. , Blaszczak E. , Jezewska-Witkowska G.

Warianty tytułu

EN

Selected parameters of validation of AmpFl STR MiniFilerTM kit used in forensic genetics

• Języki publikacji: PL

Abstrakty

EN

The aim of the presented research was to evaluate AmpFI STR MiniFilerTM kit from Applied Biosystems from the point of its reliability, limitations and usefulness in forensic genetics. Amplification products were detected in fluorescence-based automated genetic analyzer, whereas alleles were defined on the basis of the size of the internal standard. During the validation, the following parameters were determined: detection threshold and threshold determination, linearity and measuring range and sensitivity of the method. The reliable results were obtained in the range of concentrations from 2.00 ng to 0.03125 ng of template DNA in the sample. The method has a high detection rate of alleles, also in the range of 0.0625 ng to 2.0 ng of template DNA were full DNA profile. The case of “drop out” of individual alleles and the entire system for the content of 0.03125 ng of template DNA in the sample were observed. Mini Kit Filer is suitable for pure research profiles or mixtures. To a lesser extent it is suitable for the trace analysis of contact because of its high sensitivity.

Słowa kluczowe

PL

genetyka sadowa; amplifikacja DNA; zestaw AmpFl STR MiniFilerTM; walidacja; prog detekcji; prog oznaczalnosci; czulosc; niezawodnosc; liniowosc; zakres pomiarowy

• Wydawca: -
• Czasopismo: Przegląd Hodowlany
• Rocznik: 2013
• Tom: 81
• Numer: 3
• Opis fizyczny: s.6-7,rys.,tab.,bibliogr.

Twórcy

autor

Gryzinska M.

• Katedra Biologicznych Podstaw Produkcji Zwierzęcej, Uniwersytet Przyrodniczy w Lublinie, Lublin

autor

Szewczuk D.

• Komenda Wojewódzka Policji w Lublinie, Lublin

autor

Blaszczak E.

• University of Rennes

autor

Jezewska-Witkowska G.

• Katedra Biologicznych Podstaw Produkcji Zwierzęcej, Uniwersytet Przyrodniczy w Lublinie, Lublin

Bibliografia

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