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2011 | 71 | 1 |
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Migratory capabilities of human umbilical cord blood-derived neural stem cells (HUCB-NSC) in vitro

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Many types of neural progenitors from various sources have been evaluated for therapy of CNS disorders. Prerequisite for success in cell therapy is the ability for transplanted cells to reach appropriate target such as stroke lesion. We have established neural stem cell line from human umbilical cord blood neural stem (HUCB-NSC). In the present study we evaluated migratory capabilities of cells (HUCB-NSC) and the presence of various migration-related receptors. Immunocytochemical analysis revealed abundant expression of CXCR4, PDGFRa, PDGFRp, c-Met, VEGFR, IGF-1R and PSA-NCAM receptors in non-adherent population of HUCB-NSC cultured in serum free (SF) conditions (SF cells). Biological activity of selected receptors was confirmed by HUCB-NSC in vitro migration towards SDF-1 and IGF-1 ligands. Additionally, rat brain-derived homogenates have been assessed for their chemoattractive activity of HUCB-NSC. Our experiments unveiled that brain tissue was more attracted for HUCB-NSC than single ligands with higher potency of injured than intact brain. Moreover, adherent HUCB-NSC cultured in low serum (LS) conditions (LS cells) were employed to investigate an impact of different extracellular matrix (ECM) proteins on cell motility. It turned out that laminin provided most permissive microenvironment for cell migration, followed by fibronectin and gelatin. Unexpected nuclear localization of CXCR4 in SF cells prompted us to characterize intracellular pattern of this expression in relation to developmental stage of cells cultured in different conditions. Continuous culture of LS cells revealed cytoplasmatic pattern of CXCR4 expression while HUCB-NSC cultured in high serum conditions (HS cells) resulted in gradual translocation of CXCR4 from nucleus to cytoplasm and then to arising processes. Terminal differentiation of HUCB-NSC was followed by CXCR4 expression decline.
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  • Mossakowski Medical Research Centre, Department of NeuroRepair, Polish Academy of Sciences, Warsaw, Poland
  • Mossakowski Medical Research Centre, Department of NeuroRepair, Polish Academy of Sciences, Warsaw, Poland
  • Mossakowski Medical Research Centre, Department of NeuroRepair, Polish Academy of Sciences, Warsaw, Poland
  • Ali H, Bahbahani H (2010) Umbilical cord blood stem cells - potential therapeutic tool for neural injuries and disor­ders. Acta Neurobiol Exp (Wars) 70: 316-324.
  • Altenburg JD, Jin Q, Alkhatib B, Alkhatib G (2010) The potent anti-HIV activity of CXCL12gamma correlates with efficient CXCR4 binding and internalization. J Virol 84: 2563-2572.
  • Andrae J, Afink G, Zhang XQ, Wurst W, Nister M (2004) Forced expression of platelet-derived growth factor B in the mouse cerebellar primordium changes cell migration during midline fusion and causes cerebellar ectopia. Mol Cell Neurosci 26: 308-321.
  • Annenkov A (2009) The insulin-like growth factor (IGF) receptor type 1 (IGF1R) as an essential component of the signalling network regulating neurogenesis. Mol Neurobiol 40: 195-215.
  • Arcese W, Aversa F, Bandini G, De Vincentiis A, Falda M, Lanata L, Lemoli RM, Locatelli F, Majolino I, Zanon P, Tura S (1998) Clinical use of allogeneic hematopoietic stem cells from sources other than bone marrow. Haematologica 83: 159-182.
  • Barkho BZ, Munoz AE, Li X, Li L, Cunningham LA, Zhao X (2008) Endogenous matrix metalloproteinase (MMP)-3 and MMP-9 promote the differentiation and migration of adult neural progenitor cells in response to chemokines. Stem Cells 26: 3139-3149.
  • Battista D, Rutishauser U (2010) Removal of polysialic acid triggers dispersion of subventricularly derived neuro- blasts into surrounding CNS tissues. J Neurosci 30: 3995-4003.
  • Bersano A, Ballabio E, Lanfranconi S, Boncoraglio GB, Corti S, Locatelli F, Baron P, Bresolin N, Parati E, Candelise L (2010) Clinical studies in stem cells trans­plantation for stroke: a review. Curr Vasc Pharmacol 8: 29-34.
  • Bilozur ME, Hay ED (1988) Neural crest migration in 3D extracellular matrix utilizes laminin, fibronectin, or col­lagen. Dev Biol 125: 19-33.
  • Bleul CC, Fuhlbrigge RC, Casasnovas JM, Aiuti A, Springer TA (1996) A highly efficacious lymphocyte chemoattrac- tant, stromal cell-derived factor 1 (SDF-1). J Exp Med 184: 1101-1109.
  • Boncoraglio GB, Bersano A, Candelise L, Reynolds BA, Parati EA (2010) Stem cell transplantation for ischemic stroke. Cochrane Database Syst Rev 8: CD007231.
  • Buzanska L, Machaj EK, Zablocka B, Pojda Z, Domanska- Janik K (2002) Human cord blood-derived cells attain neuronal and glial features in vitro. J Cell Sci 115: 2131— 2138.
  • Buzanska L, Jurga M, Domanska-Janik K (2006a) Neuronal differentiation of human umbilical cord blood neural stem-like cell line. Neurodegener Dis 3: 19-26.
  • Buzanska L, Jurga M, Stachowiak EK, Stachowiak MK, Domanska-Janik K (2006b) Neural stem-like cell line derived from a nonhematopoietic population of human umbilical cord blood. Stem Cells Dev 15: 391-406.
  • Carbajal KS, Schaumburg C, Strieter R, Kane J, Lane TE (2010) Migration of engrafted neural stem cells is medi­ated by CXCL12 signaling through CXCR4 in a viral model of multiple sclerosis. Proc Natl Acad Sci USA 107: 11068-11073.
  • Dziembowska M, Tham TN, Lau P, Vitry S, Lazarini F, Dubois-Dalcq M (2005) A role for CXCR4 signaling in survival and migration of neural and oligodendrocyte precursors. Glia 50: 258-269.
  • Franceschini I, Vitry S, Padilla F, Casanova P, Tham TN, Fukuda M, Rougon G, Durbec P, Dubois-Dalcq M (2004) Migrating and myelinating potential of neural precursors engineered to overexpress PSA-NCAM. Mol Cell Neurosci 27: 151-162.
  • Freed CR, Greene PE, Breeze RE, Tsai WY, DuMouchel W, Kao R, Dillon S, Winfield H, Culver S, Trojanowski JQ, Eidelberg D, Fahn S (2001) Transplantation of embryonic dopamine neurons for severe Parkinson's disease. N Engl J Med 344: 710-719.
  • Garzotto D, Giacobini P, Crepaldi T, Fasolo A, De Marchis S (2008) Hepatocyte growth factor regulates migration of olfactory interneuron precursors in the rostral migratory stream through Met-Grb2 coupling. J Neurosci 28: 5901­5909.
  • Giannoudis PV, Einhorn TA, Schmidmaier G, Marsh D (2008) The diamond concept - open questions. Injury 39: S5-S8.
  • Gokhale PJ, Andrews PW (2006) A prospective on stem cell research. Semin Reprod Med 24: 289-297.
  • Habisch HJ, Fiedler J, Ludolph AC, Storch A, Brenner RE (2008) Altered migration and adhesion potential of pro- neurally converted human bone marrow stromal cells. Cytotherapy 10: 824-833.
  • Hicks A, Jolkkonen J (2009) Challenges and possibilities of intravascular cell therapy in stroke. Acta Neurobiol Exp (Wars) 69: 1-11.
  • Horner RD (1998) The high cost of stroke to society, the family, and the patient. Pharmacotherapy 18: 87-93.
  • Howard C, Murray PE, Namerow KN (2010) Dental pulp stem cell migration. J Endod 36: 1963-1966.
  • Jablonska A, Kozlowska H, Markiewicz I, Domanska-Janik K, Lukomska B (2010) Transplantation of neural stem cells derived from human cord blood to the brain of adult and neonatal rats. Acta Neurobiol Exp (Wars) 70: 337­350.
  • Janowski M, Gornicka-Pawlak E, Kozlowska H, Domanska- Janik K, Gielecki J, Lukomska B (2008) Structural and functional characteristic of a model for deep-seated lacu- nar infarct in rats. J Neurol Sci 273: 40-48.
  • Janowski M (2009) Functional diversity of SDF-1 splicing variants. Cell Adh Migr 3: 243-249.
  • Janowski M, Date I (2009) Systemic neurotransplantation - a problem-oriented systematic review. Rev Neurosci 20: 39-60.
  • Joers VL, Emborg ME (2009) Preclinical assessment of stem cell therapies for neurological diseases. Ilar J 51: 24-41.
  • Jurga M, Markiewicz I, Sarnowska A, Habich A, Kozlowska H, Lukomska B, Buzanska L, Domanska-Janik K (2006) Neurogenic potential of human umbilical cord blood: neural-like stem cells depend on previous long-term cul­ture conditions. J Neurosci Res 83: 627-637.
  • Kline RM, Bertolone SJ (1998) Umbilical cord blood trans­plantation: providing a donor for everyone needing a bone marrow transplant? South Med J 91: 821-828.
  • Kozlowska H, Jablonka J, Janowski M, Jurga M, Kossut M, Domanska-Janik K (2007) Transplantation of a novel human cord blood-derived neural-like stem cell line in a rat model of cortical infarct. Stem Cells Dev 16: 481­488.
  • Kucia M, Wojakowski W, Reca R, Machalinski B, Gozdzik J, Majka M, Baran J, Ratajczak J, Ratajczak MZ (2006) The migration of bone marrow-derived non-hematopoiet- ic tissue-committed stem cells is regulated in an SDF-1-, HGF-, and LIF-dependent manner. Arch Immunol Ther Exp (Warsz) 54: 121-135.
  • Lambert H, Dellacasa-Lindberg I, Barragan A (2011) Migratory responses of leukocytes infected with Toxoplasma gondii. Microbes Infect 13: 96-102
  • Lindvall O, Kokaia Z (2006) Stem cells for the treatment of neurological disorders. Nature 441: 1094-1096.
  • Liu XS, Chopp M, Santra M, Hozeska-Solgot A, Zhang RL, Wang L, Teng H, Lu M, Zhang ZG (2008) Functional response to SDF1 alpha through over-expression of CXCR4 on adult subventricular zone progenitor cells. Brain Res 1226: 18-26.
  • Mauney J, Olsen BR, Volloch V (2010) Matrix remodeling as stem cell recruitment event: a novel in vitro model for homing of human bone marrow stromal cells to the site of injury shows crucial role of extracellular collagen matrix. Matrix Biol 29: 657-663.
  • Muller WE (2006) The stem cell concept in sponges (Porifera): Metazoan traits. Semin Cell Dev Biol 17: 481-491.
  • Na IK, Scheibenbogen C, Adam C, Stroux A, Ghadjar P, Thiel E, Keilholz U, Coupland SE (2008) Nuclear expres­sion of CXCR4 in tumor cells of non-small cell lung cancer is correlated with lymph node metastasis. Hum Pathol 39: 1751-1755.
  • Netelenbos T, Zuijderduijn S, Van Den Born J, Kessler FL, Zweegman S, Huijgens PC, DragerAM (2002) Proteoglycans guide SDF-1-induced migration of hematopoietic progeni­tor cells. J Leukoc Biol 72: 353-362.
  • Newman MB, Willing AE, Manresa JJ, Davis-Sanberg C, Sanberg PR (2005) Stroke-induced migration of human umbilical cord blood cells: time course and cytokines. Stem Cells Dev 14: 576-586.
  • Olanow CW, Goetz CG, Kordower JH, Stoessl AJ, Sossi V, Brin MF, Shannon KM, Nauert GM, Perl DP, Godbold J, Freeman TB (2003) A double-blind controlled trial of bilateral fetal nigral transplantation in Parkinson's dis­ease. Ann Neurol 54: 403-414.
  • Peichev M, Naiyer AJ, Pereira D, Zhu Z, Lane WJ, Williams M, Oz MC, Hicklin DJ, Witte L, Moore MA, Rafii S (2000) Expression of VEGFR-2 and AC133 by circulat­ing human CD34(+) cells identifies a population of func­tional endothelial precursors. Blood 95: 952-958.
  • Rooprai HK, Liyanage K, Robinson SF, Kandanearatchi A, Dean AF, Pilkington GJ (1999) Extracellular matrix- modulated differential invasion of human meningioma cell lines in vitro. Neurosci Lett 263: 214-216.
  • Sanchez-Ramos JR, Song S, Kamath SG, Zigova T, Willing A, Cardozo-Pelaez F, Stedeford T, Chopp M, Sanberg PR (2001) Expression of neural markers in human umbilical cord blood. Exp Neurol 171: 109-115.
  • Sarnowska A, Jurga M, Buzanska L, Filipkowski RK, Duniec K, Domanska-Janik K (2009) Bilateral interac­tion between cord blood-derived human neural stem cells and organotypic rat hippocampal culture. Stem Cells Dev 18: 1191-1200.
  • Schlenska-Lange A, Knupfer H, Lange TJ, Kiess W, Knupfer M (2008) Cell proliferation and migration in glioblastoma multiforme cell lines are influenced by insulin-like growth factor I in vitro. Anticancer Res 28: 1055-1060.
  • Son BR, Marquez-Curtis LA, Kucia M, Wysoczynski M, Turner AR, Ratajczak J, Ratajczak MZ, Janowska- Wieczorek A (2006) Migration of bone marrow and cord blood mesenchymal stem cells in vitro is regulated by stromal-derived factor-1-CXCR4 and hepatocyte growth factor-c-met axes and involves matrix metalloproteinases. Stem Cells 24: 1254-1264.
  • Spano JP, Andre F, Morat L, Sabatier L, Besse B, Combadiere C, Deterre P, Martin A, Azorin J, Valeyre D, Khayat D, Le Chevalier T, Soria JC (2004) Chemokine receptor CXCR4 and early-stage non-small cell lung cancer: pattern of expression and correlation with outcome. Ann Oncol 15: 613-617.
  • Speetjens FM, Liefers GJ, Korbee CJ, Mesker WE, van de Velde CJ, van Vlierberghe RL, Morreau H, Tollenaar RA, Kuppen PJ (2009) Nuclear localization of CXCR4 deter­mines prognosis for colorectal cancer patients. Cancer Microenviron 2: 1-7.
  • Strachan LR, Condic ML (2004) Cranial neural crest recycle surface integrins in a substratum-dependent manner to promote rapid motility. J Cell Biol 167: 545-554.
  • Strachan LR, Condic ML (2008) Neural crest motility on fibronectin is regulated by integrin activation. Exp Cell Res 314: 441-452.
  • Sypecka J, Dragun-Szymczak P, Zalewska T, Domanska- Janik K (2009) Laminin promotes oligogliogenesis and increases MMPs activity in human neural stem cells of HUCB-NSC line. Acta Neurobiol Exp (Wars) 69: 37-45.
  • Szymczak P, Wojcik-Stanaszek L, Sypecka J, Sokolowska A, Zalewska T (2010) Effect of matrix metalloproteinases inhibition on the proliferation and differentiation of HUCB-NSCs cultured in the presence of adhesive sub­strates. Acta Neurobiol Exp (Wars) 70: 325-336.
  • Takizawa H, Kubo-Akashi C, Nobuhisa I, Kwon SM, Iseki M, Taga T, Takatsu K, Takaki S (2006) Enhanced engraft- ment of hematopoietic stem/progenitor cells by the tran­sient inhibition of an adaptor protein, Lnk. Blood 107: 2968-2975.
  • Tataria M, Penyman SV, Sylvester KG (2006) Stem cells: tissue regeneration and cancer. Semin Pediatr Surg 15: 284-292.
  • Tate MC, Garcia AJ, Keselowsky BG, Schumm MA, Archer DR, LaPlaca MC (2004) Specific beta1 integrins mediate adhesion, migration, and differentiation of neural pro­genitors derived from the embryonic striatum. Mol Cell Neurosci 27: 22-31.
  • Wagner PL, Hyjek E, Vazquez MF, Meherally D, Liu YF, Chadwick PA, Rengifo T, Sica GL, Port JL, Lee PC, Paul S, Altorki NK, Saqi A (2009) CXCL12 and CXCR4 in adenocarcinoma of the lung: association with metastasis and survival. J Thorac Cardiovasc Surg 137: 615-621.
  • Walczak P, Zhang J, Gilad AA, Kedziorek DA, Ruiz-Cabello J, Young RG, Pittenger MF, van Zijl PC, Huang J, Bulte
  • JW (2008) Dual-modality monitoring of targeted intraar- terial delivery of mesenchymal stem cells after transient ischemia. Stroke 39: 1569-1574.
  • Wang H, Zeng X, Fan Z, Lim B (2010) RhoH plays distinct roles in T-cell migrations induced by different doses of SDF1 alpha. Cell Signal 22: 1022-1032.
  • Wang L, Li Y, Chen J, Gautam SC, Zhang Z, Lu M, Chopp M (2002) Ischemic cerebral tissue and MCP-1 enhance rat bone marrow stromal cell migration in interface cul­ture. Exp Hematol 30: 831-836.
  • Wang L, Wang Z, Yang B, Yang Q, Wang L, Sun Y (2009) CXCR4 nuclear localization follows binding of its ligand SDF-1 and occurs in metastatic but not primary renal cell carcinoma. Oncol Rep 22: 1333-1339.
  • Wang LH, Liu Q, Xu B, Chen W, Yang Q, Wang ZX, Sun YH (2010) Identification of nuclear localization sequence of CXCR4 in renal cell carcinoma by constructing expression plasmids of different deletants. Plasmid 63: 68-72.
  • Weber K, Paulus W, Senner V (2010) The side population of gliomas exhibits decreased cell migration. J Neuropathol Exp Neurol 69: 623-631.
  • Wysoczynski M, Reca R, Ratajczak J, Kucia M, Shirvaikar N, Honczarenko M, Mills M, Wanzeck J, Janowska- Wieczorek A, Ratajczak MZ (2005) Incorporation of CXCR4 into membrane lipid rafts primes homing-related responses of hematopoietic stem/progenitor cells to an SDF-1 gradient. Blood 105: 40-48.
  • Yu T, Huang H, Li HF (2010) Stromal cell-derived fac- tor-1 promotes migration of cells from the upper rhom­bic lip in cerebellar development. J Neurosci Res 88: 2775-2786.
  • Zawadzka M, Lukasiuk K, Machaj EK, Pojda Z, Kaminska B (2009) Lack of migration and neurological benefits after infusion of umbilical cord blood cells in ischemic brain injury. Acta Neurobiol Exp (Wars) 69: 46-51.
  • Ziu M, Schmidt NO, Cargioli TG, Aboody KS, Black PM, Carroll RS (2006) Glioma-produced extracellular matrix influences brain tumor tropism of human neural stem cells. J Neurooncol 79: 125-133.
  • Zou C, Song G, Luo Q, Yuan L, Yang L (2010) Mesenchymal stem cells require integrin beta1 for directed migration induced by osteopontin in vitro. In Vitro Cell Dev Biol Anim. [Epub ahead of print].
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