Microfabrication techniques and surface functionalisation for the culture of neural stem cells

• Autorzy: Rossi F. , Colpo P. , Mehn D. , Nowak J. , Ruiz A.
• Języki publikacji: EN

Abstrakty

EN

We present different processes used for the patterning and growth of stem cells based on microspotting, microcontact printing and 3D patterning. Microspotting and microcontact-printing technique have been performed to produce micropatterned surfaces for cell-biological applications. Biomolecules have been micropsotted and microstamped on plasma-polymerized poly ethylene glycol substrates. The patterns exhibited a firm stability and an improved feasibility for controlling cell localization, proliferation and even differentiation. Production and application of 3D substrate by combination of lithography and in situ UV cross linking of photoresist are also detailed. An application of these processes is the biofunctional surfaces were incubated with suspensions of human umbilical cord blood neural stem cells (HUCB-NSC). It was clearly observed how the cells adhered and grew in the protein patterned regions. After 4 weeks of culturing the cells were still anchored in the patterns. Immunocytochemistry studies indicate that cell differentiation can be controled by the combination of interface engineering and culture conditions.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2011
• Tom: 71
• Numer: S
• Opis fizyczny: p.19

Twórcy

autor

Rossi F.

• Joint Research Centre, IHCP, Brussels, Belgium

autor

Colpo P.

• Joint Research Centre, IHCP, Brussels, Belgium

autor

Mehn D.

• Joint Research Centre, IHCP, Brussels, Belgium

autor

Nowak J.

• Joint Research Centre, IHCP, Brussels, Belgium

autor

Ruiz A.

• Department of Pharmacology, Chemotherapy and Medical Toxicology, University of Milan, Milan, Italy

Uwagi

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