Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 59 | 2 |
Tytuł artykułu

Host specificity and in vivo infectivities of the mouse coccidian parasites Eimeria krijgsmanni

Warianty tytułu
Języki publikacji
In the present study, infection experiments of E. krijgsmanni using various hosts were conducted to elucidate the host specificity among some animals and the infectivity to mouse strains. According to the results, the infection was not found in most animals, except for rats, in which some oocyst shedding was detected, and there was no significant difference in infectivity among mouse strains. Additionally, oocyst shedding was hardly detectable in a secondary infection to immunocompetent mice, although it was found in immunodeficient mice. These results indicated that only immunocompetent mice could develop adaptive immunity against reinfection by stimuli of the primary infection. Furthermore, the infection experiments were performed with splenic macrophage (Mφ)-depleted mice with a reagent and Beige (Bg) mice known to be a strain of mice with low NK cell activity. No significant effect was found in primary or secondary infections in the Mφ-depleted mice, whereas the mortality rate was clearly increased in Bg mice inoculated with a large number of oocysts. Their oocyst shedding was similar to that of immunocompetent hosts. Taken together, these results suggested that Mφ has only a minor role in the immune response, but the NK cell has an important function in resistance to primary infection of E. krijgsmanni.
Opis fizyczny
  • Laboratory of Parasitology, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan
  • Laboratory of Emerging Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan
  • Laboratory of Protozoan Diseases, National Institute of Animal Health, Tsukuba 305-0856, Japan
  • Kyorin University School of Health Science, Tokyo 192-8508, Japan
  • National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan
  • Division of Tropical Diseases and Parasitology, Kyorin University School of Medicine, Tokyo 181-8611, Japan
  • Abbas R.Z., Iqbal Z., Blake D., Khan M.N., Saleemi M.K. 2011. Anticoccidial drug resistance in fowl coccidia: the state of play revisited. World’s Poultry Science Journal, 67, 337–350. DOI: 10.1017/S004393391100033X.
  • Aguirre S.A., Mason P.H., Perryman L.E. 1994. Susceptibility of major histocompatibility complex (MHC) class I- and MHC class II-deficient mice to Cryptosporidium parvum infection. Infection and Immunity, 62, 697–699.
  • Barakat F.M., McDonald V., Di Santo J.P., Korbel D.S. 2009. Roles for NK cells and an NK cell-independent source of intestinal gamma interferon for innate immunity to Cryptosporidium parvum infection. Infection and Immunity, 77, 5044–5049. DOI: 10.1128/IAI.00377-09.
  • Chen W., Harp J.A., Harmsen A.G. 1993a. Requirements for CD4+ cells and gamma interferon in resolution of established Cryptosporidium parvum infection in mice. Infection and Immunity, 61, 3928–3932.
  • Chen W., Harp J.A., Harmsen A.G., Havell E.A. 1993b. Gamma interferon functions in resistance to Cryptosporidium parvum infection in severe combined immunodeficient mice. Infection and Immunity, 61, 3548–3551.
  • Denkers E.Y., Gazzinelli R.T. 1998. Regulation and function of T-cell-mediated immunity during Toxoplasma gondii infection. Clinical Microbiology Reviews, 11, 569–588.
  • Lou D.Q., Lesbordes J.C., Nicolas G., Viatte L., Bennoun M., Van Rooijen N., Kahn A., Renia L., Vaulont S. 2005. Iron- and inflammation-induced hepcidin gene expression in mice is not mediated by Kupffer cells in vivo. Hepatology, 41, 1056–1064. DOI: 10.1002/hep.20663.
  • Matsui T., Fujino T., Kobayashi F., Morita T., Imai S. 2006. Life cycle of Eimeria krijgsmanni-like coccidium in the mouse (Mus musculus). Journal of Veterinary Medical Science, 68, 331–336. DOI: 10.1292/jvms.68.331.
  • McDonald V., Bancroft G.J. 1994. Mechanisms of innate and acquired resistance to Cryptosporidium parvum infection in SCID mice. Parasite Immunology, 16, 315–320. DOI: 10.1111/j.1365-3024.1994.tb00354.x.
  • McDonald V., Deer R., Uni S., Iseki M., Bancroft G.J. 1992. Immune responses to Cryptosporidium muris and Cryptosporidium parvum in adult immunocompetent or immunocompromised (nude and SCID) mice. Infection and Immunity, 60, 3325–3331.
  • Pogonka T., Schelzke K., Stange J., Papadakis K., Steinfelder S., Liesenfeld O., Lucius R. 2010. CD8+ cells protect mice against reinfection with the intestinal parasite Eimeria falciformis. Microbes and Infection, 12, 218–226. DOI: 10.1016/j.micinf.2009.12.005.
  • Rose M.E., Hesketh P., Wakelin D. 1992. Immune control of murine coccidiosis: CD4+ and CD8+ T lymphocytes contribute differentially in resistance to primary and secondary infections. Parasitology, 105, 349–354. DOI: 10.1017/s0031182000074515.
  • Rose M.E., Owen D.G., Hesketh P. 1984. Susceptibility to coccidiosis: effect of strain of mouse on reproduction of Eimeria vermiformis. Parasitology, 88, 45–54. DOI: 10.1017/s0031182000054330.
  • Rose M.E., Wakelin D., Hesketh P. 1989. Gamma interferon controls Eimeria vermiformis primary infection in BALB/c mice. Infection and Immunity, 57, 1599–1603.
  • Schito M.L., Barta J.R. 1997. Nonspecific immune responses and mechanisms of resistance to Eimeria papillata infections in mice. Infection and Immunity, 65, 3165–3170.
  • Smith A.L., Hayday A.C. 2000. Genetic dissection of primary and secondary responses to a widespread natural pathogen of the gut, Eimeria vermiformis. Infection and Immunity, 11, 6273–6280. DOI: 10.1128/IAI.68.11.6273-6280.2000.
  • Takeuchi D., Jones V.C., Kobayashi M., Suzuki F. 2008. Cooperative role of macrophages and neutrophils in host antiprotozoan resistance in mice acutely infected with Cryptosporidium parvum. Infection and Immunity, 76, 3657–3663. DOI: 10.1128/IAI.00112-08.
  • Taubert A., Behrendt J.H., Sühwold A., Zahner H., Hermosilla C. 2009. Monocyte- and macrophage-mediated immune reactions against Eimeria bovis. Veterinary Parasitology, 164, 141–153. DOI: 10.1016/j.vetpar.2009.06.003.
  • Ungar B.L., Kao T.C., Burris J.A., Finkelman F.D. 1991. Cryptosporidium infection in an adult mouse model. Independent roles for IFN-gamma and CD4+ T lymphocytes in protective immunity. Journal of Immunology, 147, 1014–1022.
  • Vetterling J.M. 1976. Eimeria tenella: host specificity in gallinaceous birds. Journal of Protozoology, 23, 155–158. DOI: 10.1111/j.1550-7408.1976.tb05261.x.
  • Wallach M. 2010. Role of antibody in immunity and control of chicken coccidiosis. Trends in Parasitology, 26, 382–387. DOI: 10.1016/
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.