Microsatellite loci-based distribution of Trypanosoma cruzi genotypes from Chilean chronic Chagas disease patients and Triatoma infestans is concordant with a specific host-parasite association hypothesis

• Autorzy: Venegas J. , Diaz F. , Rojas T. , Miranda S. , Jercic M. I. , Gonzalez C. , Conoepan W. , Vargas A. , Pichuantes S. , Gajardo M. , Rodriguez J. , Sanchez G.
• Języki publikacji: EN

Abstrakty

EN

The objective of this study was to investigate if there is specific host-parasite association in Chilean populations of Trypanosoma cruzi. For this purpose, two groups of parasites were analyzed, one from chronic chagasic patients, and the other from Triatoma infestans triatomines in three regions of the country. The first group consisted of four types of samples: parasites from peripheral blood of non-cardiopathic T. cruzi infected patients (NB); parasites from their corresponding xenodiagnosis (NX); parasites from peripheral blood of T. cruzi infected cardiopathic patients (CB) and parasites from their xenodiagnostics (CX). The T. infestans sample in turn was from three regions: III, V and M (Metropolitan). The genetic differentiation by the Fisher exact method, the lineage distribution of the samples, the molecular phylogeny and the frequency of multiclonality were analysed. The results show that not only are the groups of T. cruzi clones from Chagas disease patients and vectors genetically differentiated, but also all the sub-groups (NB, NX, CB and CX) from the III, V and M regions. The analysis of lineage distribution was concordant with the above results, because significant differences among the percentages of TcI, TcIII and hybrids (TcV or TcVI) were observed. The phylogenetic reconstruction with these Chilean T. cruzi samples was coherent with the above results because the four chagasic samples clustered together in a node with high bootstrap support, whereas the three triatomine samples (III, V and M) were located apart from that node. The topology of the tree including published T. cruzi clones and isolates was concordant with the known topology, which confirmed that the results presented here are correct and are not biased by experimental error. Taken together the results presented here are concordant with a specific host-parasite association between some Chilean T. cruzi populations.

Słowa kluczowe

EN

Chagas disease; Chilean population; Trypanosoma cruzi; infection; patient; population genetics; phylogenesis; host-parasite interaction; triatomine; Triatoma infestans; phylogenetic analysis

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2013
• Tom: 58
• Numer: 2
• Opis fizyczny: p.139-148,fig.,ref.

Twórcy

autor

Venegas J.

• Programa de Biologia Celular y Molecular, Instituto de Ciencias Biomedicas, Facultad de Medicina, correo 70086, Santiago, Chile

autor

Diaz F.

autor

Rojas T.

autor

Miranda S.

autor

Jercic M. I.

autor

Gonzalez C.

autor

Conoepan W.

autor

Vargas A.

autor

Pichuantes S.

autor

Gajardo M.

autor

Rodriguez J.

autor

Sanchez G.

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