Deciphering the molecular responses to methanol-enhanced photosynthesis and stomatal conductance in broad bean

• Autorzy: Zhao Y. , Zeng Z.-D. , Qi C.-J. , Yu X.-S. , Guo C.-L. , Chen Q. , Chen L.-M.
• Języki publikacji: EN

Abstrakty

EN

This study showed that foliar spray of 2 % methanol significantly increased stomatal conductance, photosynthesis, and growth of broad bean (Vicia faba L.). To understand the molecular mechanisms behind the methanol-enhanced photosynthesis of broad bean, a forward suppression subtractive hybridization (SSH) cDNA library was constructed with 2 % methanol-sprayed leaves. A total of 145 methanol-induced genes were identified, and 63 of these had a known function. Clustering analysis indicated that the largest proportion (41.3 %) comprised genes associated with photosynthesis. RT-PCR analysis verified that the transcription of photosynthetic genes, including Rubisco and chlorophyll a/b-binding proteins of photosystem I and II, was upregulated in 2 % methanolsprayed leaves, which might contribute to the molecular mechanism of methanol-enhanced photosynthesis in broad bean. Further analysis suggested that the transcription and expression of 14-3-3 protein (present in the SSH library) and PM H⁺-ATPase encoding genes, PM H⁺-ATPase phosphorylation and its interactions with 14-3-3 proteins as well as stomatal aperture increased in leaves after 2 % methanol sprayed. Simultaneously, the leaf H₂O₂ content decreased in a time-dependent manner. Based on these results, we hypothesize that methanol spray induces the expression of 14-3-3 proteins and PM H⁺-ATPase and simultaneously reduces the H₂O₂ content in leaves. Consequently, the interaction between phosphorylated PM H⁺- ATPase and 14-3-3 proteins is enhanced, and PM H⁺- ATPase and H⁺ pump activities elevated; these functions may constitute the molecular mechanism through which methanol increases stomatal aperture and conductance in broad bean leaves.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 11
• Opis fizyczny: p.2883-2896,fig.,ref.

Twórcy

autor

Zhao Y.

• Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Chenggong, Kunming, 650500, China
• Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Chenggong, Kunming, 650500, China

autor

Zeng Z.-D.

• Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Chenggong, Kunming, 650500, China

autor

Qi C.-J.

• Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Chenggong, Kunming, 650500, China

autor

Yu X.-S.

• Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Chenggong, Kunming, 650500, China

autor

Guo C.-L.

• Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Chenggong, Kunming, 650500, China

autor

Chen Q.

• Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Chenggong, Kunming, 650500, China

autor

Chen L.-M.

• Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Chenggong, Kunming, 650500, China

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Identyfikatory

DOI

10.1007/s11738-014-1658-x