PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2017 | 21 |

Tytuł artykułu

Abundance of live and dead cells of bacterioneuston and bacterioplankton from the Slupia River estuary

Treść / Zawartość

Warianty tytułu

PL
Liczebność żywych i martwych komórek bakterioneustonu i bakterioplanktonu w estuarium rzeki Słupi

Języki publikacji

EN

Abstrakty

EN
The study was carried out in the estuarine part of the Słupia River, which, for the most part, comprises the harbour channel. The results of the present study showed that the total abundance of bacterioneuston was higher compared to bacterioplankton. In these two groups of bacteria, dead bacterial cells were dominant. The total number of bacteria, as well as the number of live and dead bacteria was similar in the entire horizontal profile. The abundance of live and dead bacteria showed distinct seasonal variation.
PL
Badania bakteriologiczne przeprowadzono w estuariowym odcinku rzeki Słupi będącym kanałem portowym. Wodę z czterech stanowisk badawczych pobierano z błony powierzchniowej oraz warstwy podpowierzchniowej. W badanych próbach przy użyciu mikroskopu epifluoroscencyjnego oznaczono ogólną liczebność bakterioneustonu i bakterioplanktonu oraz liczebność komórek bakterii żywych i martwych. Uzyskane wyniki badan wykazały, że bakterie neustonowe były liczniejsze niż bakterie planktonowe. W obu warstwach dominowały martwe komórki bakterii. W profilu horyzontalnym stwierdzono nieomal homogenne liczbowe występowanie badanych organizmów. W bakterioustonie wysoki odsetek martwych komórek, odnotowano wiosną i latem, kiedy promieniowanie UV jest najbardziej intensywne.

Wydawca

-

Rocznik

Tom

21

Opis fizyczny

p.61-72,fig.,ref.

Twórcy

autor
  • Department of Experimental Biology, Institute of Biology and Environmental Protection, Pomeranian University in Slupsk, Arciszewskiego 22b, 76-200 Slupsk, Poland
autor
  • Department of Experimental Biology, Institute of Biology and Environmental Protection, Pomeranian University in Slupsk, Arciszewskiego 22b, 76-200 Slupsk, Poland

Bibliografia

  • Alonso-Sáez L., Gasol J.M., Lefort T., Hofer J., Sommaruga R., 2006. Effect of natural sunlight on bacterial activity and differential sensivity of natural bacterioplankton groups in northwestern Mediterranean Coastal waters. Appl. Environ. Microbiol., 72, 5806-5813.
  • Berman T., Kaplan B., Chava S., Viner Y., Sherr B.F., Sherr E.B., 2001. Metabolically active bacteria in lake Kinneret. Aquat. Microb. Ecol., 23, 213-224.
  • Bogdanova O.Yu., Golovchenko A.V., Lysak L.V., Glukhova T.V., Zvyagintsev D.G., 2014. Viability of bacteria in peatlands. Eurasian Soil Sci., 47, 297-303.
  • Bratbak G., Heldal M., 2000. Viruses rule the waves – the smallest and most abundant members of ecosystems. Microbiol. Today, 27, 171-173.
  • Choi J.W., Sherr E.B., 1996. Relation between presence/absence of visible nucleoid and metabolic activity in bacterioplankton cells. Limnol. Oceanogr., 41, 1161-1168.
  • Christowa C., Luks K., Christowa-Dobrowolska M., Szulc M., Kiełb-Stańczuk M., Podruczna B., Kasperek S., Hącia E., 2007. The development strategy of the sea port in Ustka by 2021. Business Mobility International Private Limited Company, Słupsk, 19-29.
  • Cunliffe M., Engel A., Frka S., Gašparovic B., Guitart C., Murrell C., Salter M., Stolle C., Upstill-Goddard R., Wurl O., 2013. Sea surface microlayers: A unified physicochemical and biological perspective of the air–ocean interface. Prog. Oceanogr., 109, 104-116.
  • Davidson A.T., Thomson P.G., Westwood K., van den Enden R., 2004. Estimation of bacterioplankton activity in Tasmanian coastal waters and between Tasmania and Antarctica using stains. Aquat. Microb. Ecol., 37, 33-45.
  • Donderski W., Walczak M., Mudryk Z., Kobyliński M., 1999. Neustonic bacteria number, biomass and taxonomy. Pol. J. Environ. Stud., 8, 137-141.
  • Falcioni T., Papa S., Gasol J.M., 2008. Evaluating the flow-cytometric nucleic acid doublestaining protocol in realistic situations of planktonic bacterial death. Appl. Environ. Microbiol., 74, 1767-1779.
  • Freese H.M., Karsten U., Schumann R., 2006. Bacterial Abundance, Activity, and Viability in the Eutrophic River Warnow, Northeast Germany. Microb. Ecol., 51, 117-127.
  • Garrett W.D., 1965. Collection of slick-forming materials from the sea surface. Limnol. Oceanogr., 10, 602-605.
  • del Giorgio P.A., Gasol J.M., Vaqé D., Mura P., Agusti A., Duarte C.M., 1996. Protistan control of the proportion of metabolic active cells in coastal marine bacterioplankton. Limnol. Oceanogr., 41, 1169-1179.
  • Gonzáez J.M., Sherr E.B., Sherr B.F., 1993. Differential grazing by marine flagellates og growing vs starving bacteria, and on motile vs non-motile bacteria. Mar. Ecol. Prog. Ser., 102, 257-267.
  • Haglund A.L., Lantz P., Törnblom E., Tranvik L., 2003. Depth distribution of active bacteria and bacterial activity in lake sediment. FEMS Microbiol. Ecol., 46, 31-38.
  • Hervas A., Casamayor E.O., 2008. High similarity between bacterioneuston and airborne bacterial community compositions in a high mountain lake area. FEMS Microb. Ecol., 67, 219-228.
  • Hillbricht-Ilkowska A., Jasser I., Kostrzewska-Szlakowska I., 1997. Air-water interface: dynamics of nutrients and picoplankton in the surface microlayer of a humic lake. Verh. Internat. Verein. Limnol., 26, 319-322.
  • Hoppe H.G., Arnosti C., Herndel G.F., 2002. Ecological significance of bacterial enzymes in marine environment. In: Microbial enzymes in the environment activity, ecology and applications. (Eds) R.C. Burns, R.P. Dick, Marcel Dekker, Inc., New York, Basel, 73-107.
  • Kubera Ł., Donderski W., 2017. Distribution and activity of benthic bacteria in four lakes in the Bory Tucholskie National Park (Poland). Aquat. Microb. Ecol., 79, 127-135.
  • Lamy D., Artigas L.F., Jauzein C., Lizon F., Cornille V., 2006. Coastal bacterial viability and production in the eastern English Channel: A case study during a Phaeocystis globosa bloom. J. Sea Res., 56, 227-238.
  • Leuko S., Legat A., Fendrihan S., Stan-Lotter H., 2004. Evaluation of the LIVE/DEAD backlight kit for detection of extremophilic archaea and visualization of microorganisms in environmental hypersaline samples. Appl. Environ. Microbiol., 70, 6884-6886.
  • Luna G.M., Manini E., Danovaro R., 2002. Large fraction of dead and inactive bacteria in coastal Marine sediments: comparison of protocols for determination and ecological significance. Appl. Environ. Microbiol., 68, 3509-3513.
  • Maki J.S., 1993. The air-water interface as an extreme environment. In: Aquatic Microbiology. An Ecological Approach. (Ed.) T.D. Ford, Blackwell Science Publications, Boston, Oxford, London, Edinburg, Melbourne, Paris, Berlin, 409-439.
  • Mudroch A., MacKnight S.D., 1994. Handbook of Techniques for Aquatic Sediments Sampling. Second Edition CRC Press Inc., Boca Racon.
  • Mudryk Z., Donderski W., Skórczewski P., Walczak M., 1999. Neustonic and planktonic bacteria isolated from a brackish lake Gardno. Pol. Arch. Hydrobiol., 46. 121-129.
  • Mudryk Z., Korzeniewski K., Falkowska L., 1991. Bacteriological investigation of the surface microlayer of Gulf of Gdańsk. Oceanologia, 30, 93-103.
  • Mudryk Z.J., Trojanowski J., Antonowicz J., Skórczewski P., 2003. Chemical and bacteriological studies of surface and subsurface water layers in estuarine Lake Gardno. Pol. J. Environ. Stud., 12, 199-206.
  • Painchaud J., Therriault J.C., Legendre L., 1995. Assessment of Salinity-Related Mortality of Freshwater Bacteria in the Saint Lawrence Estuary. Appl. Environ. Microbiol., 61, 205-208.
  • Perliński P., Mudryk Z.J., Antonowicz J., 2017. Enzymatic activity in the surface microlayer and subsurface water in the harbour channel. Estuar. Coast. Shelf. S., 196, 150-158.
  • Perliński P., Skórczewski P., Zdanowicz M., Mudryk Z., 2015. Participation of MEM+ bacteria in the bacterioplankton community in Ustka harbor, the River Słupia estuary, Southern Baltic Sea. Eur. J. Biol. Res., 5, 17-24.
  • Quéric N-V., Soltwedel T., Arntz W.E., 2004. Application of a rapid direct viable count method to deep-sea sediment bacteria. J. Microbiol. Meth., 57, 351-367.
  • Santos A.L., Baptista I., Lopes S., Henriques I., Gomes N.C., Almeida A., Correia A., Cunha A., 2012. The UV response of bacterioneuston and bacterioplankton isolates depend on the physiological condition and involve a metabolic shift. FEMS Microbiol. Ecol., 80 (3), 646-658.
  • Santos A.L., Mendes C., Gomes N.C.M., Henriques I., Correia A., Almeida A., Cunha A., 2009. Short-term variability of abundance, diversity and activity of estuarine bacterioneuston and bacterioplankton. J. Plankton. Res., 31, 1545-1555.
  • Säwström C., Pearce I., Davidson A.T., Rosén P., Laybourn-Parry J., 2008. Influence of environmental conditions, bacterial activity and viability on the viral component in 10 Antarctic lakes. FEMS Microbiol. Ecol., 63, 12-22.
  • Senjarini K., Karsten U., Schumann R., 2013. Application of fluorescence markers for the diagnosis of bacterial abundance and viability in aquatic ecosystem. J. Microbiol. Res., 3, 143-147.
  • Stiefel P., Schmidt-Emrich S., Maniura-Weber K., Ren Q., 2015. Critical aspects of using bacterial cell viability assays with the fluorophores SYTO9 and propidium iodide. BMC Microbiol., doi: 10.1186/s12866-015-0376-x.
  • Stolle C., Nagel K., Labrenz M., Jürgens K., 2010. Succession of the sea-surface microlayer in the costal Baltic Sea under natural and experimentally induced low-wind conditions. Biogeosci., 7, 2975-2988.
  • Walczak M., 2002. Bakterie neustonowe jeziora Jeziorak Mały, występowanie, właściwości fizjologiczne i aktywność metaboliczna. (Neustonic bacteria of lake Jeziorak Mały, presence, physiological and metabolic activity). Ph.D. thesis. Nicolaus Copernicus University, Toruń, (in Polish).
  • Walczak M., Donderski W., 2005. Bacterioneuston of water reservoirs. Post. Mikrobiol., 44, 275-288.
  • Wurl O., Obbard J.P., 2004. A review of pollutants in the sea-surface microlayer (SML): a unique habitat for marine organisms. Mar. Pollut. Bull., 48, 1016-1030.
  • Yokomaku D., Yamaguchi N., Nasu M., 2000. Improved direct viable count procedure for quantitative estimation of bacterial viability in freshwater environments. Appl. Environ. Microbiol., 66, 5544-5548.
  • Zampino D., Zaccone R., La Ferla R., 2004. Determination of living and active bacterioplankton: a comparison of methods. Chem. Ecol., 20, 411-422.
  • Zdanowicz M., 2009. Bakterioneuston i bakterioplankton przymorskiego jeziora Dołgie Wielkie – występowanie, produkcja i aktywność metaboliczna. (Bacterioneuston and bacterioplankton of the coastal lake Dołgie Wielkie – occurrence, production and metabolic activity). Ph.D. thesis, Pomeranian University in Słupsk, (in Polish).
  • Zimmermann R., Meyer-Reil A., 1974. A new method for fluorescence staining of bacterial populations on membrane filters. Kiel Meer., 30, 24-27.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-b3c52e71-2f2e-4b6b-88df-3665155cbdb4
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.