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2014 | 36 | 03 |
Tytuł artykułu

Formation and stability of polysomes and polysomal populations in roots of germinating seeds of soybean (Glycine max L.) under cold, osmotic and combined cold and osmotic stress conditions

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Abiotic stress factors such as extreme temperatures or osmotic stress are among the major causes of inferior crop yields. In response to a stress, plants have evolved various defense mechanisms. In our study, we have demonstrated how cold stress, osmotic stress and a combination of both stresses retard the growth of roots and inhibit the process of ribosomes binding into polysomes. The tested stresses also limited the ability of root tissues to synthesize proteins. At the same time, most of the analyzed samples were found to contain elevated shares of the fractions of cytoskeleton-bound polysomes (CBP, CMBP) in the total population of polysomes. Using a polysome-based degradation system, it was shown that polysomes formed under stress conditions were much more resistant to the effect of exogenous ribonuclease than the control ones. The highest tolerance to digestion was demonstrated by the cytoskeleton-bound (CBP) and cytoskeleton-membrane bound polysomes (CMBP). The increasing share of CBP and their stability in roots of seeds germinating under stress conditions can be a target for physiological regulation. It seems that modifications in the stability and percentages of particular polysomal populations play an important role in the adaptation of plants to stress conditions, which may indicate that these forms of polysomes, i.e., cytoskeleton-bound ones, are involved (via selective translation) in the synthesis of stress proteins in soybean roots.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
36
Numer
03
Opis fizyczny
p.651-662,fig.,ref.
Twórcy
autor
  • Department of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Street 1a, 10-957, Olsztyn, Poland
  • Research and Education Center ‘Laboratory of Molecular Diagnostics’, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Prawochenskiego Street 5, 10-720, Olsztyn, Poland
autor
  • Department of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Street 1a, 10-957, Olsztyn, Poland
autor
  • Department of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Street 1a, 10-957, Olsztyn, Poland
autor
  • Department of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Street 1a, 10-957, Olsztyn, Poland
autor
  • Department of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Street 1a, 10-957, Olsztyn, Poland
Bibliografia
  • Abe S, Davies E (1991) Isolation of F-actin from pea stems: evidence from fluorescence microscopy. Protoplasma 163:51–61.
  • Abe S, Ito Y, Davies E (1992) Co-sedimentation of actin, tubulin and membranes in the cytoskeleton fractions from peas and mouse 3T3 cells. J Exp Bot 43:941–949.
  • Abe S, Azama K, Sugimoto H, Davies E (2003) Protein accumulation in the maize endosperm: role of the polyribosomes and the cytoskeleton. Plant Physiol Biochem 41:125–131.
  • Bailey-Serres J (1998) Cytoplasmic ribosomes of higher plants. In: J. Bailey-Serres, D. Gallie, eds. A look beyond transcription: mechanisms determining mRNA stability and translation in plants. American Society of Plant Physiology, Rockville, pp 125–144.
  • Bensen RJ, Boyer JS, Mullet JE (1988) Water deficit-induced changes in ABA, growth, polysomes and translatable RNA in soybean hypocotyls. Plant Physiol 88:289–294.
  • Bewley JD, Bradford KJ, Hilhorst HWM, Nanogaki H (2013) Seeds: physiology of development, germination and dormancy. Springer, New York.
  • Brosowska-Arendt W, Weidner S (2011) Effect of osmotic stress on the formation of a population of polysomes and their stability in pea (Pisum sativum L.) seeds. Acta Physiol Plant 33:1475–1482.
  • Brosowska-Arendt W, Weidner S (2012) Effect of osmotic stress on in vitro translational capacity of polysomes and on the composition of polysome-associated proteins in germinating seeds of pea (Pisum sativum L.). Acta Soc Bot Pol 81:185–191.
  • Cánovas FM, Dumas-Gaudot E, Recorbet G, Jorrin J, Mock HP, Rossignol M (2004) Plant proteome analysis. Proteomics 4:285–298.
  • Chiba Y, Green Y (2009) mRNA degradation machinery in plants. J Plant Biol 52:114–124.
  • Crosby JS, Vayda ME (1991) Stress-induced translational control in potato tubers may be mediated by polysome-associated proteins. Plant Cell 3:1013–1023.
  • Davies E, Abe S (1995) Method for isolation and analysis of polyribosomes. Methods Cell Biol 50:209–222.
  • Davies E, Fillingham BD, Abe S, Ito Y (1991) Evidence for the existence of cytoskeleton-bound polysomes in plants. Cell Biol Int 15:973–981.
  • Davies E, Comer EC, Lionberger JM, Stankovic B, Abe S (1993) Cytoskeleton-bound polysomes in plants. III. Polysome–cytoskeleton-membrane interaction in maize. Cell Biol Int Rep 17:331–340.
  • Davies E, Abe S, Larkins B, Clore A, Quatrano R, Weidner S (1998) The role of the cytoskeleton in protein synthesis. In: Bailey-Serres J, Gallie D, eds. A look beyond transcription: mechanisms determining mRNA stability and translation in plants. American Society of Plant Physiology Rockville, pp 115–124.
  • Eulgem T (2005) Regulation of Arabidopsis defense transcriptome. Trends Plant Sci 10:71–78.
  • Falk H (1969) Rough thylakoids: polysomes attached to chloroplast membranes. J Cell Biol 42:582–587.
  • Funatsuki HH, Kurosaki T, Murakami S, Matsuba K, Kawaguchi S, Yumoto S, Sato Y (2003) Deficiency of a cytosolic ascorbate peroxidase associated with chilling tolerance in soybean. Theor Appl Genet 106:494–502.
  • Gardner JC, Payne TL (2003) A soybean biotechnology outlook. AgBioForum 6:1–3.
  • Green PJ (1993) Control of mRNA stability in higher plants. Plant Physiol 102:1065–1070.
  • Gualerzi C, Cammarano P (1969) Comparative electrophoretic studies on the protein of chloroplast and cytoplasmic ribosomes of spinach leaves. Biochim Biophys Acta 190:170–186.
  • Guhaniyogi J, Brewer G (2001) Regulation of mRNA stability in mammalian cells. Gene 265:11–23.
  • Han Y, Yu J, Guo F, Watkins SC (2006) Polysomes are associated with microtubules in fertilized eggs of Chinese pine (Pinus tabulaeformis). Protoplasma 227:223–227.
  • Kawaguchi R, Williams A, Bray E, Bailey-Serres J (2003) Waterdeficit-induced translational control in Nicotiana tabacum. Plant Cell Environ 26:221–229.
  • Kawaguchi R, Girke T, Bray EA, Bailey-Serres J (2004) Differential mRNA translation contributes to gene regulation under nonstress and dehydration stress conditions in Arabidopsis thaliana. Plant J 38:823–839.
  • Klyachko NL, Aksenowa L, Dunaeva M, Kulikova AL (2000) Interaction of plant polysomes with the action cytoskeleton. Cell Biol Int 24:351–358.
  • Klyachko NL, Kulikova AL, Erokhima MA (2003) Plant polysome binding to the actin cytoskeleton as a target for physiological regulation. Cell Biol Int 27:217–218.
  • Knapińska AM, Irizarry-Barreto P, Adsunmalli S, Androulakis I, Brewer G (2005) Molecular mechanisms regulating mRNA stability: physiological and pathological significance. Curr Genomics 6(6):1–16.
  • Kosowska M, Frączek E, Amarowicz R, Karamać M, Abe S, Weidner S (2004) Water-deficit-induced changes in cytoskeleton-bound and other polysomal populations in embryonic tissue during triticale caryopsis germination. Acta Physiol Plant 26:67–74.
  • Kulikova AL, Kulikov AY, Erohina MA, Klyachko NL (2001) The percentage of cytoskeleton-bound polysomes as affected by the physiological condition of plant cells. Russ J Plant Physiol 48(5):608–614.
  • Laroche A, Hopkins WG (1987) Polysomes from winter rye seedlings grown at low temperature. Plant Physiol 85:648–654.
  • Munro AJ, Jackson RJ, Korner A (1964) Studies on the nature of polysomes. Biochem J 92:289–299.
  • Raven PH, Evert RF, Eichhorn SE (2005) Biology of plants, 7th edn. Freeman Worth, New York.
  • Sami-Subbu R, Muench DG, Okita TW (2000) A cytoskeleton-associated RNA-binding protein binds to the untranslated regions of prolamine mRNA and to poly(A). Plant Sci 152: 115–122.
  • Slaymaker D, Hoppey C (2006) Reduced polysome levels and preferential recruitment of a defense gene transcript into polysomes in soybean cells treated with the syringolide elicitor. Plant Sci 170:54–60.
  • Szypulska E, Weidner S (2011) Importance of cytomatrix-bound polysomes to synthesis of lysine-containing proteins in triticale germs under ABA treatment. Acta Physiol Plant 33:1461–1465.
  • Weidner S, Łukaszewicz D (1997) The role of free, membrane-bound and tightly bound polysomes in precocious germination of triticale caryopses. Acta Physiol Plant 19:109–118.
  • Weidner S, Frączek E, Romanowska M, Amarowicz R, Abe S, Davies E (2003) The influence of abscisic acid on different polysomal populations in embryonic tissue during pea seeds germination. Acta Physiol Plant 25(1):5–12.
  • Weidner S, Każarnowicz M, Frączek E, Amarowicz R, Karamać M (2006) Exogenous abscisic acid increases stability of polysomes in embryos of triticale caryopses during germination. Acta Physiol Plant 28(6):627–634.
  • Weidner S, Kordala E, Karamać M, Kosińska A, Amarowicz R (2009) Phenolic compounds and properties of antioxidants in grapevine roots (Vitis vinifera L.) under low temperature stress followed by recovery. Acta Physiol Plant 4:279–286.
  • Wróbel M, Karamać M, Amarowicz R, Frączek E, Weidner S (2005) Metabolism of phenolic compounds in Vitis riparia seeds during stratification and during germination under optimal and low temperature stress conditions. Acta Physiol Plant 27(3A): 313–320.
  • Zak EA, Sokolov OI, Greengauz OK, Bocharova MA, Klyachko NL (1997) Polysomes from Vicia faba L., leaves bound to the actin cytoskeleton. J Exp Bot 48(310):1019–1026.
  • Zambetti G, Wilming L, Fey EG, Penman S, Stein J, Stein G (1990) Differential association of membrane-bound and non-membrane-bound polysomes with the cytoskeleton. Exp Cell Res 191: 246–255.
  • Zanetti ME, Chang IF, Gong F, Galbraith DW, Bailey-Serres J (2005) Immunopurification of polyribosomal complexes of Arabidopsis for global analysis of gene expression. Plant Physiol 138: 624–635.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-b3c3ebb7-9912-4452-b888-be9bafd39e3f
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