PCR-based detection of Helicobacter pylori and non-Helicobacter pylori species among humans and animals with potential for zoonotic infections

• Autorzy: Youssef A.I. , Afifi A. , Abbadi S. , Hamed A. , Enany M.
• Języki publikacji: EN

Abstrakty

EN

Helicobacter species have been reported in animals, some of which are of zoonotic importance. This study aimed to detect Helicobacter species among human and animal samples using conventional PCR assays and to identify their zoonotic potentials. Helicobacter species was identified in human and animal samples by genus-specific PCR assays and phylogenetic analysis of partial sequencing of the 16S ribosomal RNA gene. The results revealed that elicobacter species DNA was detected in 13 of 29 (44.83%) of the human samples. H. pylori was identified in 2 (15.38%), and H. bovis was detected in 4 (30.77%), whereas 7 (53.85%) were unidentified. H. bovis and H. heilmannii were prevalent among the animal samples. Phylogenetic analysis revealed bootstrapping of sequences with H. cinaedi in camel, H. rappini in sheep and humans, and Wollinella succinogenes in humans. In conclusion, the occurrence of non-H. pylori infections among human and animal samples suggested zoonotic potentials.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2021
• Tom: 24
• Numer: 3
• Opis fizyczny: p.445-450,fig.,ref.

Twórcy

autor

Youssef A.I.

• Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, Suez Canal University, 41522, 4.5 Km Ring Road, Ismailia, Egypt

autor

Afifi A.

• Microbiology and Immunology Department, Faculty of Veterinary Medicine, Suez Canal University, Egypt 3 Microbiology and Immunology Department, Faculty of Medicine, Suez University, 43512, Alsalam City, Suez, Egypt

autor

Abbadi S.

• Microbiology and Immunology Department, Faculty of Medicine, Suez University, 43512, Alsalam City, Suez, Egypt

autor

Hamed A.

• Biotechnology Department, Animal Health Research Institute, P.O. Box 264, Dokki, Giza 12618, Egypt

autor

Enany M.

• Microbiology and Immunology Department, Faculty of Veterinary Medicine, Suez Canal University, Egypt

Identyfikatory

DOI

10.24425/pjvs.2021.138737