An immunohistochemical analysis of lymphocytic infiltrations in canine skin cancers

• Autorzy: Madej J.A. , Madej J.P. , Dzimira S. , Nowak M.
• Języki publikacji: EN

Abstrakty

EN

Lymphocytic infiltrations located in the extracellular matrix often accompany canine skin cancer. They can be characterised as an inflammatory infiltration and/or a second tumour – lymphoma. The aim of this study was an immunohistochemical analysis of a lymphocytic infiltration which accompanies spontaneous skin cancer. Twenty basal cell carcinoma, 20 non-keratinizing squamous cell carcinoma, 20 keratinizing squamous cell carcinoma and 8 sebaceous gland carcinoma samples which were accompanied by a lymphocytic infiltration and/or secondary lymphatic follicles were verified histopathologically. The expression of bcl-2, CD3, CD79α, Ki-67, MCM-3 and MCM-7 in the lymphocytic infiltration was evaluated. Four types of lymphocytic infiltrations were found: I – diffuse bcl-2⁺, II – diffuse bcl-2⁻, III – follicular bcl-2⁺/⁻ where the centre was bcl-2⁻, and the marginal zone of the follicles and the extrafollicular area were bcl-2⁺ and IV – aggregated bcl-2⁺, where the centre and periphery were bcl-2⁺. The I and IV type corresponds to lymphoma, II type is non-neoplastic immune response and III type suggest reactive follicular hyperplasia. The proliferation of lymphocytes which demonstrated the expression of neoplastic markers (I and IV), suggests preneoplastic phase (pseudolymphoma) or lymphoma – the second independent tumour. A high proliferative index of the follicular blc-2⁺/⁻ follicular infiltration indicates an increased immunological response of the host against skin cancer.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2017
• Tom: 20
• Numer: 1
• Opis fizyczny: p.141-147,fig.,ref.

Twórcy

autor

Madej J.A.

• Division of Pathomorphology and Veterinary Forensics, Department of Pathology, Wroclaw University of Environmental and Life Sciences, Norwida 31, 50-375 Wroclaw, Poland

autor

Madej J.P.

• Division of Histology and Embryology, Department of Animal Physiology and Biostructure, Wroclaw University of Environmental and Life Sciences, Norwida 31, 50-375 Wroclaw, Poland

autor

Dzimira S.

• Division of Pathomorphology and Veterinary Forensics, Department of Pathology, Wroclaw University of Environmental and Life Sciences, Norwida 31, 50-375 Wroclaw, Poland

autor

Nowak M.

• Division of Pathomorphology and Veterinary Forensics, Department of Pathology, Wroclaw University of Environmental and Life Sciences, Norwida 31, 50-375 Wroclaw, Poland

Bibliografia

• Affolter VK, Moore PF (2000) Canine cutaneous and systemic histiocytosis: reactive histiocytosis of dermal dendritic cells. Am J Dermatopathol 22: 40-48.
• Aparicio OM, Weinstein DM, Bell SP (1997) Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase. Cell 91: 59-69.
• Aster J (2013) Hematopoietic and lymphoid systems. In: Kumar V, Abbas A, Aster J (eds) Robbins Basic Pathology. 9th ed., Saunders, PA, Philadelphia, pp 407-458.
• Basingab FS, Ahmadi M, Morgan DJ (2016) IFNγ-Dependent Interactions between ICAM-1 and LFA-1 Counteract Prostaglandin E2-Mediated Inhibition of Antitumor CTL Responses. Cancer Immunol Res 4: 400-411.
• Carbone A, Della Libera D, Zannier L, Selva A, Ceolin P, Gualeni A, Canal B, Gloghini A (2011a) In situ follicular lymphoma associated with overt B- or T-cell lymphomas in the same lymph node. Am J Hematol 86: E66-70.
• Carbone A, Gloghini A (2011) Coexisting follicular and mantle cell lymphoma with each having an in situ component. Am J Clin Pathol 136: 481-483.
• Carbone A, Tibiletti MG, Canzonieri V, Rossi D, Perin T, Bernesconi B, Gloghini A (2011b) In situ follicular lymphoma associated with nonlymphoid malignancies. Leuk Lymphoma 53: 603-608.
• Cong P, Raffeld M, Teruya-Feldstein J, Sorbara L, Pittaluga S, Jaffe ES (2002) In situ localization of follicular lymphoma: description and analysis by laser capture microdissection. Blood 99: 3376-3382.
• Copelan EA, McGuire EA (1995) The biology and treatment of acute lymphoblastic leukemia in adults. Blood 85: 1151-1168.
• Correia C, Lee SH, Meng XW, Vincelette ND, Knorr KL, Ding H, Nowakowski GS, Dai H, Kaufmann SH (2015) Emerging understanding of Bcl-2 biology: Implications for neoplastic progression and treatment. Biochim Biophys Acta 1853: 1658-1671.
• Cupedo T, Mebius RE (2005) Cellular interactions in lymph node development. J Immunol 174: 21-25.
• García-Muñoz R, Feliu J, Llorente L (2015) The top ten clues to understand the origin of chronic lymphocytic leukemia (CLL). J Autoimmun 56: 81-86.
• Hsiao YW, Liao KW, Hung SW, Chu RM (2004) Tumor-infiltrating lymphocyte secretion of IL-6 antagonizes tumor-derived TGF-beta 1 and restores the lymphokine-activated killing activity. J Immunol 172: 1508-1514.
• Lupi O (2007) Correlations between the Sonic Hedgehog pathway and basal cell carcinoma. Int J Dermatol 46: 1113-1117.
• Madej JA (2015) Pathomorphology and pathogenesis of very rare groups of tumors. Med Weter 71: 264-275.
• Matsuzawa T, Inozume T, Takaki M, Harada K, Kawamura T, Shibagaki N, Shimada S (2015) Case of folliculotropic mycosis fungoides with prominent loss of T-cell antigens CD7 and CD26 in blood T cells. J Dermatol 42: 108-109.
• MacKie RM (2002) Skin cancer. Martin Dunitz, London.
• Montes-Moreno S, Castro Y, Rodriguez-Pinilla SM, Garcia JF, Mollejo M, Castillo ME, Bas-Vernal A, Barrionuevo-Cornejo C, Sanchez-Verde L, Menarguez J, Cigudosa JC, Piris MA (2010) Intrafollicular neoplasia/in situ follicular lymphoma: review of a series of 13 cases. Histopathology 56: 658-662.
• Musahl C, Holthoff HP, Lesch R, Knippers R (1998) Stability of the replicative Mcm3 protein in proliferating and differentiating human cells. Exp Cell Res 241: 260-264.
• Nybakken GE, Bala R, Gratzinger D, Jones CD, Zehnder JL, Bangs CD, Cherry A, Warnke RA, Natkunam Y (2016) Isolated Follicles Enriched for Centroblasts and Lacking t(14;18)/BCL2 in Lymphoid Tissue: Diagnostic and Clinical Implications. PLoS One 11: e0151735.
• Ralfkiaer E, Stein H, Bosq J, Gatter KC, Ralfkiaer N, Wantzin GL, Mason DY (1986) Expression of a cell-cycle-associated nuclear antigen (Ki-67) in cutaneous lymphoid infiltrates. Am J Dermatopathol 8: 37-43.
• Rebhun RB, Thamm DH (2010) Multiple distinct malignancies in dogs; 53 cases. J Am Anim Hosp Assoc 46: 20-30.
• Roullet MR, Martinez D, Ma L, Fowler MH, McPhail ED, Judkins A, Arber DA, Bagg A (2010) Coexisting follicular and mantle cell lymphoma with each having an in situ component: A novel, curious, and complex consultation case of coincidental, composite, colonizing lymphoma. Am J Clin Pathol 133: 584-591.
• Scholzen T, Gerdes J (2000) The Ki-67 protein: from the known and the unknown. J Cell Physiol 182: 311-322.
• Shelton RM (2001) Skin cancer: a review and atlas for medical provider. Mt Sinai J Med 68: 243-252.
• Watanabe R, Nanko H, Fulkuda S (2006) Lymphoma cutis due to pierced earrings. J Cutan Pathol 33: 16-19.

Identyfikatory

DOI

10.1515/pjvs-2017-0018