Molekularna identyfikacja gatunków z rodzaju Abies na podstawie polimorfizmu DNA mitochondrialnego

• Autorzy: Pawlaczyk E.M. , Staniak J. , Malinski T. , Bobowicz M.A.

Warianty tytułu

EN

Molecular identification of species from Abies genus based on the mitochondrial DNA polymorphism

• Języki publikacji: PL

Abstrakty

EN

The plant material was collected on 34 individuals growing in the Dendrological Garden of Poznań University of Life Sciences (52°25'32,95" N 16°53'39,83" E) and Botanical Garden of Adam Mickiewicz University in Poznań (52°25'11,70" N 16°52'55,07" E). The species for this study originated from Europe, Asia Minor, central and eastern Asia and North America and included: Abies alba, Abies cephalonica, Abies cilicica, Abies equi−trojani, Abies sibirica, Abies koreana, Abies pinsapo, Abies ×insignis, Abies bornmulleriana, Abies homolepsis, Abies holophylla, Abies grandis, Abies concolor, Abies concolor var. violacea, Abies concolor var. lowiana, Abies nordmanniana, Abies ×arnoldiana, Abies nephrolepis and Abies balsamea. The aim of this study was to define the species haplotypes (the length of allele) on the basis of nad5−4 mitochondrial DNA marker detected by capillary electrophoresis. This marker has been suggested as an easy−to−use tool to distinguish species of the Abies genus and it could be species−specific. Seven different haplotypes were identified. The first one appears in the species from Europe, Asia and North America. The second one was detected in firs from Europe and Asia Minor. A. cephalonica and A. sibirica were identified by the third haplotype, which occurs also in A. alba from the Balkan region. The fourth haplotype is characteristic for species from Asia and North America. The fifth and sixth haplotypes were identified in A. pinsapo and A. numidica. The seventh haplotype was detected only in A. holophylla. Applied marker is a very useful for verification of fir species especially allopatric species, less for parapatric ones. This marker is more helpful to exclude the species than to precisely identify them.

Słowa kluczowe

PL

lesnictwo; drzewa lesne; genetyka roslin; jodla; Abies; gatunki roslin; identyfikacja; haplotypy; DNA mitochondrialny; polimorfizm DNA

• Wydawca: -
• Czasopismo: Sylwan
• Rocznik: 2015
• Tom: 159
• Numer: 08
• Opis fizyczny: s.675-683,fot.,rys.,tab.,bibliogr.

Twórcy

autor

Pawlaczyk E.M.

• Zakład Genetyki, Uniwersytet im.Adama Mickiewicza w Poznaniu, ul.Umultowska 89, 61-614 Poznań

autor

Staniak J.

• Zakład Genetyki, Uniwersytet im.Adama Mickiewicza w Poznaniu, ul.Umultowska 89, 61-614 Poznań

autor

Malinski T.

• Katedra Botaniki Leśnej, Uniwersytet Przyrodniczy w Poznaniu, ul.Wojska Polskiego 71 D, 60-625 Poznań

autor

Bobowicz M.A.

• Zakład Genetyki, Uniwersytet im.Adama Mickiewicza w Poznaniu, ul.Umultowska 89, 61-614 Poznań

Bibliografia

• Boratyński A. 1983. Systematyka i geograficzne rozmieszczenie. W: Nasze drzewa leśne. Jodła pospolita Abies alba Mill. PWN, Warszawa – Poznań. 41-85.
• Doyle J. J., Doyle J. L. 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13-15.
• Farjon A. 2010. A Handbook of the World's Conifers. Leiden, Netherlands: Brill Academic Publishers.
• Farjon A., Rushforth K. D. 1989. A classification of Abies Miller (Pinaceae). Notes of the Royal Botanic Garden Edinburgh 46 (1): 59-79.
• Gömöry D., Longauer R., Liepelt S., Ballian D., Brus R., Kraigher H., Parpan V. I., Parpan T. V., Paule L., Stupar V., Ziegenhagen B. 2004. Variation patterns of mitochondrial DNA of Abies alba Mill. in suture zones of postglacial migration in Europe. Acta Societatis Botanicorum Poloniae 73 (3): 203-206.
• Liepelt S., Bialozyt R., Ziegenhagen B. 2002. Wind-dispered pollen mediates postglacial gene flow among refugia. The Proceedings of National Academy of Science USA 99: 14590-14594.
• Liepelt S., Chedaddi R., de Beaulieu J.-L., Fady B., Gömöry D., Hussendorfer E., Konnert M., Litt T., Longauer R., Terhürne-Berson R., Ziegenhagen B. 2009. Postglacial range expansion and its genetic imprints in Abies alba (Mill.) – a synthesis from paleobotanic and genetic data. Review of Palaeobotany and Palynology 153: 139-149.
• Pawlaczyk E. M., Kroplewska I., Bobowicz M. A. 2013. Postglacjalna migracja jodły pospolitej (Abies alba Mill.) do Polski – analiza na podstawie polimorfizmu mitochondrialnego DNA. Sylwan 157 (6): 458-463.
• Ziegenhagen B., Fady B., Kuhlenkamp V., Liepelt S. 2005. Differentiating groups of Abies species with a simple molecular marker. Silvae Genetica 54 (3): 123-126.