Detection of classical genes and enterotoxins of Staphylococcus aureus isolated from raw milk in the south-east region of Poland

• Autorzy: Korpysa-Dzirba W. , Osek J.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to investigate if the enterotoxigenic strains of S. aureus isolated from raw milk are able to produce staphylococcal enterotoxins (SEs) A - E. A total of 168 of S. aureus isolates from raw milk collected in the south - east region of Poland (Lubelskie Province) were tested for SE production by the ELFA, while multiplex PCR was applied for detection of enterotoxin genes (sea, seb, sec, sed, see). It was found that 20 (11.9%) out of 168 strains were positive for one or more classical SE markers and 19 of them produced a detectable level of enterotoxins. The results obtained by mPCR and ELFA were in agreement, when the presence of A, B, and D toxin types was tested; whereas SEC was not found by the ELFA although the S. aureus was positive for the respective gene. The results of the two methods showed that mPCR identified one more strain potentially producing enterotoxin than the ELFA, which may suggest that the enterotoxigenic S. aureus are not always able to express the toxin protein.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2014
• Tom: 58
• Numer: 4
• Opis fizyczny: p.559-561,ref.

Twórcy

autor

Korpysa-Dzirba W.

• Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Osek J.

• Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

• 1. Adesiyum A.A., Tatini S.R., Hoover D.G.: Production of enterotoxin by Staphylococcus hyicus. Vet Microbiol 1984, 9, 487-495.
• 2. Bendahou A., Abid M., Bouteldoun N., Catelejine D., Lebbadi M.: Enterotoxigenic coagulase positive Staphylococcus in milk and milk products, Iben and jben, in northern Morocco. J Infect Dev Ctries 2009, 3, 169-176.
• 3. Bergdoll M.S.: Staphylococcus aureus. In: Foodborne pathogens, edited by M.P. Doyle, New York, 1989, pp.139-162.
• 4. Borelli B.M., Ferreira E.G., Lacerda I.C.A., Santos D.A., Carmo L.S., Dias R.S., Silva M.C.C., Rosa C.A.: Enterotoxigenic Staphylococcus spp. and other microbial contaminants during production of Canastra Cheese. Braz J Microbiol 2006, 37, 545-560.
• 5. Bystroń J., Bania J., Lis E., Molenda J., Bednarski M.: Characterization of Staphylococcus aureus strains isolated from cow's milk. Bull Vet Inst Pulawy 2009, 53, 59-63.
• 6. Bystroń J., Molenda J., Bania J., Czerw M.: Enterotoxigencity of staphylococci isolated from some foodstuffs of animal origin. Acta Sci Pol Med Vet 2005, 4, 95-103.
• 7. Cremonesi P., Luzzana M., Brasca M., Morandi S., Lodi R., Vimercati C., Agnellini D., Caramenti G., Moroni P., Castiglioni B.: Development of a multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products. Mol Cell Probes 2005, 19, 299-305.
• 8. D'Amico D.J., Groves E., Donnelly C.W.: Low incidence of foodborne pathogens of concern in raw milk utilized for fannstead cheese production. J Food Prot 2008, 71, 1580-1589.
• 9. Dinges M.M., Orwin P.M., Schlievert P.M.: Exotoxins of Staphylococcus aureus. Clin Microbiol Rev 2000, 13, 16-34.
• 10. EFSA (European Food Safety Authority) and ECDC (European Centre for Disease Prevention and Control), 2014. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2012. EFSA J 2014, 12, 3547.
• 11. Evenson M., Hinds M., Bernstein R., Bergdoll M.: Estimation of human dose of staphylococcal enterotoxin-A from a large outbreak of staphylococcal food poisoning involving chocolate milk. Int J Food Microbiol 1988, 7, 311-316.
• 12. Jablonsky L.M., Bohach G.A.: Staphylococcus aureus. In: Food Microbiology: Fundamentals and Frontiers, edited by M.P. Doyle, L. R. Beuchat, T.J. Montville, ASM Press, Washington, 1997, pp. 353-375.
• 13. Jorgensen H.J., Mork T., Hogasen H.R., Rorvik L.M.: Enterotoxigenic Staphylococcus aureus in bulk milk in Norway. J Appl Microbiol 2005, 99, 158-166.
• 14. Jorgensen H.J., Mork T., Rorvik L.M.: The occurrence of Staphylococcus aureus on a farm with small-scale production of raw milk cheese. J. Dairy Sci 2005, 88, 3810-3817.
• 15. Kerouanton A., Hennekinne J.A., Letertre C., Petit L., Chesneau O., Brisabois A., De Buyser M.L.: Characterization of Staphylococcus aureus strains associated with food poisoning outbreaks in France. Int J Food Microbiol 2007, 115, 369-375.
• 16. Korpysa-Dzirba W., Osek J.: Identification of genes encoding classical staphylococcal enterotoxins in Staphylococcus aureus isolated from raw milk. Bull Vet Inst Pulawy 2011, 55, 55-58.
• 17. Loncarevic S., Jorgensen H.J., Lovseth A., Mathisen T., Rorvik L.M.: Diversity of Staphylococcus aureus enterotoxin types within single samples of raw milk and raw milk products. J Appl Microbiol 2005, 98, 344-350.
• 18. Morandi S., Brasca M., Lodi R., Cremonesi P., Castiglioni B.: Detection of classical enterotoxins and identification of enterotoxin genes in Staphylococcus aureus from milk and daily products. Vet Microbiol 2007, 124, 66-72.
• 19. Orwin P.M., Leung D.Y., Donahue H.L., Novick R.P., Schlievert P.M.: Biochemical and biological properties of staphylococcal enterotoxin K. Infect Immun 2001, 69, 360-366.
• 20. Orwin P.M., Fitzgerald J.R., Leung, D.Y., Gutierrez J.A., Bohach G.A., Schlievert P.M.: Characterization of Staphylococcus aureus enterotoxin L. Infect Immun 2003, 71, 2916-2919.
• 21. Pereira V., Lopes C., Castro A., Silva J., Gibbs P., Teixeira P.: Characterization for enterotoxin production, virulence factors, and antibiotic susceptibility of Staphylococcus aureus isolates from various foods in Portugal. Food Microbiol 2009, 26, 278-282.
• 22. Rall V.L., Vieira F.P., Rall R., Vieitis R.L., Fernandes A., Candeias J.M., Cardoso K.F., Araújo J.P.: PCR detection of staphylococcal enterotoxin genes in Staphylococcus aureus strains isolated from raw and pasteurized milk. Vet Microbiol 2008, 132, 408-413.
• 23. Ren K., Bannan J.D., Pancholi V., Cheung A.L., Robbins J.C., Fischetti V.A., Zabriskie J.B.: Characterization and biological properties of a new staphylococcal exotoxin. J Exp Med 1994, 180, 1675-1683.
• 24. Schlegelová J., Babák V., Klímová E., Lukásová J., Navrátilová P., Sustácková A., Sedivá I., Rysánek D.: Prevalence of and resistance to anti-microbial drugs in selected microbial species isolated from bulk milk samples. J Vet Med B 2002, 49, 216-225.