Subdiaphragmatic vagotomy does not protect against the increase of noradrenergic response of the rat

• Autorzy: Wieczorek M. , Kobrzycka A. , Hubner M , Oclon E. , Zubel J. , Swiergiel A.
• Języki publikacji: EN

Abstrakty

EN

BACKGROUND AND AIMS: Peripheral administration of gram-negative bacteria endotoxin – lipopolisaccharide (LPS) is known to activate the hypothalamo-pituitary adrenal axis (HPAA) and brain noradrenergic systems. We studied the vagotomized rats responses to peripherally administered LPS using the HPLC-ED to measure the concentration of noradrenaline and their metabolite MHPG in various brain regions. METHODS: Rats were submitted to subdiaphragmatic vagotomy and after 30 days we used them for experiments. They were injected with saline (100 μl ip) and LPS (10 μg/100 μl ip) in random order, and two hours after injection they were euthanized. They brains where removed from the skull and we isolated the hypothalamus, amygdala, prefrontal medial cortex, hippocampus periaqueductal gray matter and the brainstem and submitted for chromatographic analysis. RESULTS: Future chromatographic analysis indicates, that subdiaphragmatic vagotomy did not protect against increase of noradrenaline concentration in analyzed brain regions. In case of LPS injected animals we observed increased noradrenaline concentration versus saline injected ones. These results were comparable with those observed in sham operated rats. CONCLUSIONS: There results suggest that there may be compensatory mechanisms, responsible for transferring of immune signal to the brain, and develop during such a long time of recovery after subdiaphragmatic vagotomy. Supported by NCN, grant no. UMO-2012/07/B/NZ4/00205

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.S47

Twórcy

autor

Wieczorek M.

• Department of Neurobiology, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland

autor

Kobrzycka A.

• Department of Neurobiology, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland

autor

Hubner M

• Department of Neurobiology, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland

autor

Oclon E.

• Animals Physiology and Endocrinology, Agriculture University, Krakow, Poland

autor

Zubel J.

• Animals Physiology and Endocrinology, Agriculture University, Krakow, Poland

autor

Swiergiel A.

• Faculty of Biology, University of Gdansk, Gdansk, Poland