Molecular identification of Buxtonella sulcata from associated-diarrhea in water buffaloes (Bubalus bubalis) in the Philippines

• Autorzy: Dianso J.A. , Garcia G.G. , Belotindos L.P. , Mingala C.N.
• Języki publikacji: EN

Abstrakty

EN

Sixty suspected protozoan oocysts were demonstrated from 260 fecal samples collected from water buffaloes aged one month to seven years old with clinical signs of diarrhea in four provinces in the Philippines after conventional methods of isolation, sporulation, morphological characteristics and Kinyoun Acid Fast Staining techniques. The recovered protozoan oocysts were subjected to molecular analysis. Amplification of DNA extracted from recovered Eimeria oocysts using universal primers for the ITS-1 region of 18S rRNA revealed PCR products with 348 bp size demonstrated by samples collected from Benguet, La Union and Nueva Ecija provinces in the Philippines while DNA extracted from oocysts of suspected Cryptosporidium spp. samples that applied primers for the SSU of 18S rRNA registered PCR products but no genes were amplified from diarrheic water buffaloes from these provinces. Alignment of the DNA sequences of the suspected Eimeria and Cryptosporidium species revealed sequences for three isolates of Buxtonella sulcata with product lengths that varied from 235 to 252 bp. This is an initial observation on the involvement of B. sulcata in diarrhea condition of water buffaloes in the Philippines. Phylogenetic analysis of the three local isolates of B. sulcata revealed no similarity with other protozoan constructed according to Neighbor-Joining method.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2018
• Tom: 64
• Numer: 2
• Opis fizyczny: p.93-100,fig.,ref.

Twórcy

autor

Dianso J.A.

• Department of Veterinary Studies, College of Veterinary Science and Medicine, Central Luzon State University, Science City of Munoz 3120, Nueva Ecija, Philippines

autor

Garcia G.G.

• Department of Veterinary Studies, College of Veterinary Science and Medicine, Central Luzon State University, Science City of Munoz 3120, Nueva Ecija, Philippines

autor

Belotindos L.P.

• Biosafety and Environment Section, Philippine Carabao Center National Headquarters and Gene Pool, Science City of Munoz 3120, Nueva Ecija, Philippines

autor

Mingala C.N.

• Department of Veterinary Studies, College of Veterinary Science and Medicine, Central Luzon State University, Science City of Munoz 3120, Nueva Ecija, Philippines

Bibliografia

• [1] Borghese A. 2005. Buffalo Production and Research. FAO Ed. REU Technical Series 67: 1315. http://www.fao.org/3/a-ah847e.pdf
• [2] Uriyapongson S. 2013. Buffalo and buffalo meat in Thailand. Buffalo Bulletin 32: 329-332.
• [3] Luseba D. 2005. Diarrhoea in cattle. Department of Agriculture, Republic of South Africa. http://nda.agric.za/docs/Infopaks/Diarrhoea_cattle.pdf
• [4] Al-Zubaidi M.T., Al-Mayah K.S.H. 2011. Prevalence of Buxtonella sulcata in neonatal and young calves in Al-Nasir station and some regions in Baghdad. Iraqi Journal of Science 52: 420-424.
• [5] Kočiš J., Ilić T., Becskei Z., Radisavljević K., Dimitrijević S. 2014. Buxtonellosis and coccidiosis of cattles in Northern Serbia. Acta Parasitologica 60: 158-163. doi:10.1515/ap-2015-0022
• [6] Henderson G. 2011. Calf Scours: Causes, prevention and treatment. https://www.drovers.com/article/ calfscours-causes-prevention-and-treatment-0
• [7] Kawahara F., Zhang G., Mingala C.N., Tamura Y., Koiwa M., Onuma M., Nunoya T. 2010. Genetic analysis and development of species-specific PCR assays based on ITS-1 region of rRNA in bovine Eimeria parasites. Veterinary Parasitology 174: 49-57. doi:10.1016/j.vetpar.2010.08.001
• [8] Berto B.P., McIntosh D., Lopes C.W.G. 2014. Studies on coccidian oocysts (Apicomplexa: Eucoccidiorida). Brazilian Journal of Veterinary Parasitology 1: 1-15. doi:10.1590/S1984-29612014001
• [9] Carvalho F.S., Wenceslau A.A., Teixeira M., Albuquerque G.R. 2011. Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus. Genetics and Molecular Research 10: 996-1005. doi:10.4238/vol10-2gmr1043
• [10] Mugridge N.B., Morrison D.A., Jäkel T., Heckeroth A.R., Tenter A.M., Johnson A.M. 2000. Effects of sequence alignment and structural domains of ribosomal DNA on phylogeny reconstruction for the protozoan family sarcocystidae. Molecular Biology and Evolution 17:1842-1853. doi:10.1093/oxfordjournals. molbev.a026285
• [11] Hansen J., Perry B. 1994. The epidemiology, diagnosis and control of helminth parasites of ruminants. International Laboratory for Research on Animal Diseases, Nairobi, Kenya. http://www.fao.org/Wairdocs/ILRI/x5492E/x5492e00.htm#Contents
• [12] Domingo C.Y.J. 2009. Prevalence and risk factors of zoonotic protozoa among small holder farms in Aurora Province. Doctoral Dissertation, University of the Philippines, College of Public Health, Manila, Philippines.
• [13] Dibner J., Kitchell M.L., Pfannenstiel M.A. 2002. Viability assay for sporocyst-forming protozoa. US6344340B1 https://patents.google.com/patent/US6344340
• [14] Richter B., Nedorost N., Maderner A., Weissenbök H. 2011. Detection of Cryptosporidium species in feces or gastric contents from snakes and lizards as determined by polymerase chain reaction analysis and partial sequencing of the 18S ribosomal RNA gene. Journal of Veterinary Diagnostic Investigation 23: 430-435. doi:10.1177/1040638711403415
• [15] Ho C.S., Martens W., Schook L.B., Smith D.M. 2006. Characterization of swine leukocyte antigen polymorphism by sequence-based and PCR-SSP methods in Meishan pigs Immunogenetics 58: 873-882. doi:10.1007/s00251-006-0145-y
• [16] Florião M.M., Lopes Bdo B., Berto B.P., Lopes C.W.G. 2015. New approaches for morphological diagnosis of bovine Eimeria species: a study on a subtropical organic dairy farm in Brazil. Tropical Animal Health and Production 48: 577-584. doi:10.1007/s11250-016-0998-5
• [17] Levine N.D. 1984. Taxonomy and review of the coccidian genus Cryptosporidium (Protozoa, Apicomplexa). Journal of Protozoology 31: 94-98.
• [18] Villanueva M.A., Domingo C.Y.J., Abes N.S., Mingala C.N. 2010. Incidence and risk factors of Cryptosporidium spp. infection in water buffaloes confined in a communal management system in the Philippines. Internet Journal of Veterinary Medicine 8: 1-10.
• [19] Pyziel A.M., Demiaszkiewicz A.W. 2015. Observation on sporulation of Eimeria bovis (Apicomplexa: Eimeriidae) from the European bison Bison bonasus: effect of temperature and potassium dichromate solution. Folia Parasitologica 62: 2015.020. doi:10.14411/fp.2015.020
• [20] Long P.L., Joyner L.P. 1984. Problems in the identification of species of Eimeria. Journal of Protozoology 31: 535-541.
• [21] Chiari L., Valle J.V.R., Resende R.M.S. 2009. Comparação de três métodes de extração de DNA genômico para análises moleculares em Stylosanthes guianensis. Embrapa Gado de Corte Circular Tecnica 36: 1-6 (in Portuguese).
• [22] Romano E., Brasileiro A.C.M. 1999. Extração de DNA de plants: soluções para problemas comumente encontrados. Biotecnologia 2: 40-43 (in Portuguese).
• [23] Adamska M., Leonska-Duniec A., Sawczuk M., Maciejewska A., Skotarczak B. 2012. Recovery of Cryptosporidium from skipped water and stool samples measured by PCR and real time PCR. Veterinarni Medicina 57: 224-232. https://doi.org/10.1051/parasite/2011184341
• [24] Couto M.C.M, Sudre A.P., Lima M.F., Bomfim T.C.B. 2013. Comparison of techniques for DNA extraction and agarose gel staining of DNA fragments using samples of Cryptosporidium. Veterinarni Medicina 58: 535-542. doi:10.17221/7085-VETMED
• [25] Al-Jubory Q.J.A., Al-Rubaie H.M.A. 2016. Molecular identification and characterization and phylogenetic study of six Eimeria species in cattle in Al-Najaf province. Euphrates Journal of Agriculture Science 8: 83-96.
• [26] Zainab S.R., Shahzad M.I., Mustafa M.Z., Arshad M., Ruby T. 2016. Prevalence of Eimeria bovis in cattles of Cholistan desert, Pakistan. Journal of Biodiversity and Environmental Sciences 9: 94-98.
• [27] Abeywardena H., Jex A.R., von Samson-Himmelstjerna G., Haydon S.R., Stevens M.A., Gasser R.B. 2013. First molecular characterisation of Cryptosporidium and Giardia from Bubalus bubalis (water buffalo) in Victoria, Australia. Infection, Genetics and Evolution 20: 96-102. doi:10.1016/j.meegid.2013.07.019
• [28] Al-Saffar T.M., Suliman E.G., Al-Bakri H.S. 2010. Prevalence of intestinal ciliate Buxtonella sulcata in cattle in Mosul, Iraq. Journal of Veterinary Science 24: 27-30.
• [29] Hasheminasab S.S., Moradi P., Talvar H.M., Wright I., Darbandi M.S. 2015. Buxtonella spp. like infection in cattle. Annals of Parasitology 61: 247-251. doi:10.17420/ap6104.14.
• [30] Tomczuk K., Kurek L., Stec A., Studzińska M., Mochol J. 2005. Incidence and clinical aspects of colon ciliate Buxtonella sulcata infection in cattle. Bulletin of the Veterinary Institute in Pulawy 49: 29-33.
• [31] Taylor M.A., Coop R.L., Wall R.L. 2007. Veterinary Parasitology. 3rd ed. Blackwell Publishing, London, United Kingdom.
• [32] Grim J.N., Pomajbíková K.J., Gordo F.P. 2015. Light microscopic morphometrics, ultrastructure, and molecular phylogeny of the putative pycnotrichid ciliate, Buxtonella sulcata. European Journal of Protistology 52: 425-436. http://dx.doi.org/10.1016/j.ejop.2015.06.003
• [33] Omeragić J., Crnkić C. 2015. Diarrhoea in cattle caused by Buxtonella sulcata in Sarajevo area. Veterinaria 64: 50-54.
• [34] Tung K.C., Huang C.C., Pan C.H., Yang C.H., Lai C.H. 2012. Prevalence of gastrointestinal parasites in yellow cattle between Taiwan and its offshore islands. Thai Journal of Veterinary Medicine 42: 219-224.
• [35] Urman H.D., Kelly G.W. 1964. Buxtonella sulcata. A ciliate associated with ulcerative colitis in a cow and prevalence of infection in Nebraska cattle. Iowa State University Veterinarian 26: 11.
• [36] Skotarczak B. 1997. Bacterial flora in acute and symptom-free balantidiosis. Acta Parasitologica 42: 230-233.
• [37] Hawash Y. 2014. DNA extraction from protozoan oocysts/cysts in feces for diagnostic PCR. Korean Journal of Parasitology 52: 263-271. doi:10.3347/kjp.2014.52.3.263
• [38] Pomajbíková K., Oborník M., Horák A., Petrželková K.J., Grim J.N., Levecke B., Todd A., Mulama M., Kiyang J., Modrý D. 2013. Novel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates. PLOS Neglected Tropical Diseases 7: 1-10. https://doi.org/10.1371/journal.pntd.0002140

Identyfikatory

DOI

10.17420/ap6402.139