Dependence of colonization of the large intestine by Candida on the treatment of Crohn’s disease

• Autorzy: Kowalska-Duplaga K. , Krawczyk A. , Sroka-Oleksiak A. , Salamon D. , Wedrychowicz A. , Fyderek K. , Gosiewski T.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to determine if there are quantitative differences in Candida fungi between pediatric patients with Crohn’s disease (before and after exclusive enteral nutrition (EEN), and the biologic therapy with anti-tumor necrosis factor alpha – (IFX)), and healthy controls. DNA was isolated from fecal samples and PCR was used to determine the number of fungal cells. Both therapeutic interventions resulted in a statistically significant decrease in Pediatric Crohn’s Disease Activity Index. The numbers of Candida decreased during both therapeutic intervention but the difference was statistically significant for the IFX intervention only (p = 0.045). Moreover, fungi population in both study groups declined during intervention when compared to the control group but the difference was significant before treat-ment only in the IFX group (p = 0.013). The total distribution of Candida with both IFX and EEN as well as in the control group differed significantly (p = 0.01) before treatment only. No correlation between the numbers of Candida and disease activity as well as the following biochemical parameters: serum iron concentration, protein or glucose level were found. It cannot be ruled out that, in combination with genetic and immunological disorders, fungi can contribute to the initiation of the disease process and perpetuation of active inflammation.

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2019
• Tom: 68
• Numer: 1
• Opis fizyczny: p.121-126,fig.,ref.

Twórcy

autor

Kowalska-Duplaga K.

• Department of Pediatrics, Gastroenterology and Nutrition, Faculty of Medicine, Jagiellonian University Medical College, Krakow, Poland

autor

Krawczyk A.

• Chair of Microbiology, Department of Molecular Medical Microbiology, Faculty of Medicine, Jagiellonian University Medical College, Krakow, Poland

autor

Sroka-Oleksiak A.

• Chair of Microbiology, Department of Molecular Medical Microbiology, Faculty of Medicine, Jagiellonian University Medical College, Krakow, Poland
• Chair of Microbiology, Department of Mycology, Faculty of Medicine, Jagiellonian University Medical College, Krakow, Poland

autor

Salamon D.

• Chair of Microbiology, Department of Molecular Medical Microbiology, Faculty of Medicine, Jagiellonian University Medical College, Krakow, Poland

autor

Wedrychowicz A.

• Department of Pediatrics, Gastroenterology and Nutrition, Faculty of Medicine, Jagiellonian University Medical College, Krakow, Poland

autor

Fyderek K.

• Department of Pediatrics, Gastroenterology and Nutrition, Faculty of Medicine, Jagiellonian University Medical College, Krakow, Poland

autor

Gosiewski T.

• Chair of Microbiology, Department of Molecular Medical Microbiology, Faculty of Medicine, Jagiellonian University Medical College, Krakow, Poland

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Identyfikatory

DOI

10.21307/pjm-2019-014