Selected steps of swine ICSI method to overcome seasonal effect and achieve acceptable early embryonic development, a preliminary study

• Autorzy: Tsakmakidis, L.A. , Tsantarliotou M. , Michos I.A , Basioura A.G. , Tsousis G. , Boscos C.M.
• Języki publikacji: EN

Abstrakty

EN

Porcine intra cytoplasmic sperm injection’s (ICSI) efficacy by selected protocol steps was investigated. Three trials per year’s period (hot, medium, cold) were carried out. Only large size follicles (6-8mm) were aspirated, brilliant cresyl blue (BCB) test was performed and only the BCB+ oocytes were in vitro maturated (40h) and involved to ICSI process. The presumptive embryos were in vitro cultured (15h). Raw boar semen and SpermCatch® as slowing medium were used. No differences were observed between periods regarding early embryonic development and maturation competence. ICSI achieves acceptable porcine early embryonic development rates under the investigated conditions.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2017
• Tom: 20
• Numer: 3
• Opis fizyczny: p.607-609,fig.,ref.

Twórcy

autor

Tsakmakidis, L.A.

• Farm Animals Clinic, School of Veterinary Medicine, Aristotle University of Thessaloniki, St. Voutyra 11, 56427, Thessaloniki, Greece

autor

Tsantarliotou M.

• Farm Animals Clinic, School of Veterinary Medicine, Aristotle University of Thessaloniki, St. Voutyra 11, 56427, Thessaloniki, Greece

autor

Michos I.A

• Farm Animals Clinic, School of Veterinary Medicine, Aristotle University of Thessaloniki, St. Voutyra 11, 56427, Thessaloniki, Greece

autor

Basioura A.G.

• Farm Animals Clinic, School of Veterinary Medicine, Aristotle University of Thessaloniki, St. Voutyra 11, 56427, Thessaloniki, Greece

autor

Tsousis G.

• Farm Animals Clinic, School of Veterinary Medicine, Aristotle University of Thessaloniki, St. Voutyra 11, 56427, Thessaloniki, Greece

autor

Boscos C.M.

• Farm Animals Clinic, School of Veterinary Medicine, Aristotle University of Thessaloniki, St. Voutyra 11, 56427, Thessaloniki, Greece

Bibliografia

• Almiñana C, Gil MA, Cuello C, Roca J, Vazquez JM, Rodriguez-Martinez H, Martinez EA (2005) Adjustments in IVF system for individual boars: value of additives and time of sperm-oocyte co-incubation. Theriogenology 64: 1783-1796.
• García-Roselló E, Coy P, Garcia Vázquez FA, Ruiz S, Matás C (2006a) Analysis of different factors influencing the intracytoplasmic sperm injection (ICSI) yield in pigs. Theriogenology 66: 1857-1865.
• García-Roselló E, Matás C, Cánovas S, Moreira PN, Gadea J, Coy P (2006b) Influence of sperm pretreatment on the efficiency of intracytoplasmic sperm injection in pigs. J Androl 27: 268-275.
• Lasiene K, Gedrimas V, Vitkus A, Glinskyte S, Lasys V, Valanciute A, Sienkiewicz W (2013) Evaluation of morphological criteria of sperm quality before in vitro fertilization and intracytoplasmic sperm injection. Pol J Vet Sci 16: 773-785.
• Roca J, Martinez E, Vazquez JM, Lucas X (1998) Selection of immature pig oocytes for homologous in vitro penetration assays with the brilliant cresyl blue test. Reprod Fertil Dev 10: 479-485.

Identyfikatory

DOI

10.1515/pjvs-2017-0075