PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2015 | 18 | 4 |

Tytuł artykułu

Application of real - time PCR for evaluation of distribution of equine herpesvirus type 1 in tissues of aborted fetuses

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
A highly sensitive and specific real-time PCR assay was used for detection and quantitation of equine herpesvirus type 1 (EHV-1) in the different internal organs of aborted fetuses. Tissue samples from 23 aborted fetuses submitted to the Department of Virology of the National Veterinary Research Institute in Pulawy between 2012 and 2013 were used for testing. Total DNA was extracted using a phenol-chloroform-isoamyl alcohol standard protocol. A real-time PCR with forward and reverse primers encompassing a highly conserved region encoding viral glycoprotein B was adapted for diagnosis of EHV-1 infection. The detection limit of the assay was shown to be 6.0x100 of viral DNA copies and the obtained standard curve exhibited a linear range from 100 to 107 molecules. Sixteen out of twenty three aborted fetuses (69.5%) were positive for EHV-1 in real- time PCR. The highest EHV-1 DNA load was obtained for liver (mean Ct value: 15.7) and lung (18.2) samples, while the lowest was in the thymus (29.6) and placenta (28.4).

Słowa kluczowe

Wydawca

-

Rocznik

Tom

18

Numer

4

Opis fizyczny

p.833-839,fig.,ref.

Twórcy

autor
  • Department of Virology, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland
autor
  • Department of Virology, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland
  • Department of Virology, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland

Bibliografia

  • Abdelgawad A, Azab W, Damiani AM, Baumgartner K, Will H, Osterrieder N, Greenwood AD (2014) Zebra-borne equine herpesvirus type 1 (EHV-1) infection in non-African captive mammals. Vet Microbiol 169: 102-106.
  • Allen GP (2002) Epidemic disease caused by Equine herpesvirus-1: recommendations for prevention and control. Equine Vet Educ 14: 136-142.
  • Allen GP, Bryans JT (1986) Molecular epizootiology, pathogenesis, and prophylaxis of equine herpesvirus-1 infections. Prog Vet Microbiol Immunol 2: 78-144.
  • Borchers K, Slater J (1993) A nested PCR for the detection and differentiation of EHV-1 and EHV-4. J Virol Methods 45: 331-336.
  • Diallo IS, Hewitson G, Wright L, Rodwell BJ, Corney BG (2006) Detection of equine herpesvirus type 1 using a real-time polymerase chain reaction. J Virol Methods 131: 92-98.
  • Edington N, Smyth B, Griffiths L (1991) The role of endothelial cell infection in the endometrium, placenta and foetus of equid herpesvirus 1 (EHV-1) abortions. J Comp Pathol 104: 379-387.
  • Elia G, Decaro N, Martella V, Campolo M, Desario C, Lorusso E, Cirone F, Buonavoglia C (2006) Detection of equine herpesvirus type 1 by real-time PCR. J Virol Methods 133: 70-75.
  • Foote CE, Love DN, Gilkerson JR, Wellington JE, Whalley JM (2006) EHV-1 and EHV-4 infection in vaccinated mares and their foals. Vet Immunol Immunopathol 111: 41-46.
  • Galosi CM, Vila Roza MV, Oliva GA, Pecoraro MR, Echeverria MG, Corva S, Etcheverrigaray ME (2001) A polymerase chain reaction for detection of equine herpesvirus-1 in routine diagnostic submissions of tissues from aborted foetuses. J Vet Med B Infect Dis Vet Public Health 48: 341-346.
  • Gardiner DW, Lunn DP, Goehring LS, Chiang YW, Cook C, Osterrieder N, McCue P, Del Piero F, Hussey SB, Hussey GS (2012) Strain impact on equine herpesvirus type 1 (EHV-1) abortion models: viral loads in fetal and placental tissues and foals. Vaccine 30: 6564-6572.
  • Gerst S, Borchers K, Gower SM, Smith KC (2003) Detection of EHV-1 and EHV-4 in placental sections of naturally occurring EHV-1 and EHV-4-related abortions in the UK: use of the placenta in diagnosis. Equine Vet J 35: 430-433.
  • Goodman LB, Wimer C, Dubovi EJ, Gold C, Wagner B (2012) Immunological correlates of vaccination and infection for equine herpesvirus 1. Clin Vaccine Immunol 19: 235-241.
  • Hornyák A, Bakonyi T, Kulik M, Kecskeméti S, Rusvai M (2006) Application of polymerase chain reaction and virus isolation techniques for the detection of viruses in aborted and newborn foals. Acta Vet Hung 54: 271-279.
  • Hussey SB, Clark R, Lunn KF, Breathnach C, Soboll G, Whalley JM, Lunn DP (2006) Detection and quantification of equine herpesvirus-1 viremia and nasal shedding by real-time polymerase chain reaction. J Vet Diagn Invest 18: 335-342.
  • Mackay IM (2004) Real-time PCR in the microbiology laboratory. Clin Microbiol Infect 10: 190-212.
  • Paillot R, Case R, Ross J, Newton R, Nugent J (2008) Equine Herpes Virus-1: Virus, Immunity and Vaccines. The Open Vet Sci J 2: 68-91.
  • Prickett ME (1970) The pathology of disease caused by equine herpesvirus 1. In: Bryans JT, Gerber H (eds) Equine infectious diseases II. S. Karger, Basel, pp 24-33.
  • Rimstad E, Evensen Ø (1993) The identification of equid herpesvirus 1 in paraffin-embedded tissues from aborted fetuses by polymerase chain reaction and immunohistochemistry. J Vet Diagn Invest 5: 174-183.
  • Smith KC, Whitwell KE, Blunden AS, Bestbier ME, Scase TJ, Geraghty RJ, Nugent J, Davis- Poynter NJ, Cardwell JM (2004) Equine herpesvirus-1 abortion: atypical cases with lesions largely or wholly restricted to the placenta. Equine Vet J 36: 79-82.
  • Van Maanen C (2002) Equine herpesvirus 1 and 4 infections: an update. Vet Q 24: 57-78.
  • Wohlsein P, Lehmbecker A, Spitzbarth I, Algermissen D, Baumgärtner W, Böer M, Kummrow M, Haas L, Grummer B (2011) Fatal epizootic equine herpesvirus 1 infections in new and unnatural hosts. Vet Microbiol 149: 456-460.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-b0168ad8-1401-48d5-839c-2b597eb76f3a
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.