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1996 | 43 | 4 |

Tytuł artykułu

Influence of low temperature on the synthesis of some Galleria mellonella proteins

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
In developing Gallería mellonella larvae (reared at 30°C) three proteins of 74/ 76 and 81/82 kDa were identified. They represent a group of storage proteins (LHP proteins). In Galleria larvae, the development of which is arrested by low temperature (18°C)/ accumulation of the 74, 76 and 81/82 kDa proteins was detected in the hemolymph. The synthesis of 74 kDa and 76 kDa proteins started after 24 h, and that of about 80 kDa after 96 h following the transfer of larvae from 30°C to 18°C. 20-Hydroxyecdysone inhibited synthesis of the 74 and 76 kDa proteins in larvae exposed to low temperature. The arrest of development of Galleria larvae is associated with the synthesis and accumulation of storage proteins, and ecdysteroids are involved in these processes.

Wydawca

-

Rocznik

Tom

43

Numer

4

Opis fizyczny

p.639-644,fig.

Twórcy

  • Polish Academy of Sciences, A.Pawinskiego 5a, 02-106 Warsaw, Poland
autor
autor
autor

Bibliografia

  • 1. Chippendale, G.M. (1988) Role of proteins in insect diapause; in Endocrinological Frontiers in Physiological Insect Ecology (Sehnal, F., Zabza, A. & Denlinger, D.L., eds.) pp. 331-346, Wroclaw Technical University Press, Wroclaw, Poland.
  • 2. Peferoen, M., Stynen, D. & dc Loof, A. (1982) A re-examination of the protein pattern of the hemolymph of Lqitinotarsa decemlineata, with special reference to vitellogenins and diapause proteins. Comp. Biochem. Physiol. 72B, 345-351.
  • 3. Dillwith, J.W., Lenz, C.J. & Chippendale, G.M. (1985) An immunochemical study of the dia­pause-associated protein of the south- western corn borer, Diatraea grandiosella. Insect Biochem. 15,711-722.
  • 4. Sonobe, H. & Odake, H. (1986) Studies on cmbryonic diapause in the pnd mutant of the silkworm, Bombyx moń. V. Identification of a pnd" gene-specific protein. Roux's Arch. Dev. Biol. 195,229-235.
  • 5. Joplin, K.H., Yocum, G.D. & Denlinger, D.L. (1990) Diapause specific proteins expressed by the brain during the pupal diapause of the flesh fly, Sarcophaga crassipalpis. ]. Insect Physiol. 36, 775-783.
  • 6. Ichimori, T., Ohtomo, R-, Suzuki, K. & Kurihara, M. (1990) Specific protein related to adult dia­pause in the leaf beetle, Gastrophysa actrocyanea. J. Insect Physiol. 36, 85-91.
  • 7. śula,J., Kodrik, D. & Socha, R. (1995) Hexameric haemolymph protein related to adult diapause in the red firebug, Pyrrhocoris apterus (L.) (Heteroptera). J. Insect Physiol. 41,793-800.
  • 8. Miura, K., Chirizei,Y., Shinoda,T. & Numata, 11. (1991) Cvanoprotein: quantitative changes and synthesis in diapause and juvenile hormone analog treated bean bug, Riptortus clavatns. Insect Biochem. 21, 553-562.
  • 9. Delinger, D.L. (1985) Hormonal control of dia­pause; in Comprehensive Insect Physiology, Bio­chemistry and Pharmacology (Kerkut, G.A. & Gilbert, L.I., eds.) vol. 8, pp. 353-412, Pergamon Press, Oxford.
  • 10. Cymborowski, B., Śmietanko, A. & Delbecque, J.P. (1989) Orcadian modulation of ecdysteroid titer in Calleria mellonella larvae. Comp. Biochem. Physiol. 94A, 431-438.
  • 11. Cymborowski, B., Muszyńska-Pytel, M., Por- cheron, P. & Cassier, P. (1991) Haemolymph ecdysteroid titers controlled by a cireadian clock mechanism in larvae of the wax moth, Galleria mellonella. J. Insect Physiol. 37, 35-40.
  • 12. Mikołajczyk, P. & Cymborowski, B. (1993) Lower temperature influences developmental rhythms of the wax moth Galleria mellonella: Putative role of ecdysteroids. Comp. Biochem. Physiol. 105A, 57-66.
  • 13. Sehnal, F. (1966) Kritisches Studium der Bio- nomie und Biometrie der in Verschiedenen I ebensbedingungen Gezüchteten Waschmotte Galleria mellonella (Lepidoptera). 7. Wiss. YjooI. 174, 53-82.
  • 14. Miller, S.G. &Silhacek, D.L.. (1982) Identification and purification of storage proteins in tissues of the greater wax moth Galleria mellonella (L.). Insect Biochem. 12,277-292.
  • 15. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72,248-254. 16. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227,680-685.
  • 17. Laskey, R.A. & Mills, A.D. (1975) Quantitative film detection of 3H and 14C in polyacrylamidc gels by fiuorography. Eur. J. Biochem. 56, 335- -341.
  • 18. Miller, S.G. & Silhacek, D.L. (1982) The synthesis and uptake of haemolymph storage proteins by the fat body of the greater wax moth Galleria niellonella (L.) Insect Biochem. 12,293-300.
  • 19. Kay, A., Memmel, N.A., Orchekowski, R.P. & Kumaran, A.K. (1987) Isolation of two cDNA clones coding for larval hemolymph proteins of Galleria mellonella. Insect Biochem. 17, 603-617.
  • 20. Telfer, W.H. & Kunkel, J.G. (1991) The function and evolution of insect storage hexamers. Annu. Rev. Entomol. 36,205-228.
  • 21. Kumaran, A.K., Ray, A., Tertadian, J.A. & Memmel, N.A. (1987) Effects of juvenile hor­mone, ecdystcroids and nutrition on larval hemolymph protein gene expression in Galleria mellonella. Insect Biochem. 17, 1053-1058.
  • 22. Ray, A., Memmel, N.A. & Kumaran, A.K. (1987) Developmental regulation of the larval hemo­lymph protein genes in Galleria mellonella. Roux's Arch. Dev. Biol. 196,414-420.
  • 23. Memmel, N.A. & Kumaran, A.K. (1988) Role of ecdystcroids and juvenile hormone fn regu­lation of larval haemolymph protein gene expression in Galleria mellonella. J. Insect Physiol. 34,585-591.

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Bibliografia

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