Determining the viability of zebra mussel [Dreissena polymorpha] spermatozoa and changes in the integrity of its plasma membrane using the fluorescence method

• Autorzy: Bialkowska J , Demianowicz W , Glogowski J
• Języki publikacji: EN

Abstrakty

EN

The viability of zebra mussel (Dreissena polymorpha) sperm and changes in the integrity of its plasma membrane were examined using combined nucleic fluorescent stains SYBR-14 and propidium iodide. Approximately 97 ± 3% of the sperm in freshly ejaculated zebra mussel semen was stained with SYBR-14 (potentially viable). A statistically significant decrease in plasma membrane integrity was observed after 48 hours (8°C) when 42 ± 11% of the sperm stained as viable. Semen stored at room temperature (20°C) lost its plasma membrane integrity after 4 hours (0% viable). Motility declined from 60 ± 4% (0 time) to 30 ± 3% after 24 hours. The percentage of sperm stained with SYBR-14 was not correlated with sperm motility or the fertility test.

PL

Zastosowano fluorescencyjną metodę SYBR-14/jodek propydyny (tzw. metoda żywe/martwe) w celu określenia zmian w integralności plazmolemy plemników Dreissena polymorpha. W świeżo pozyskanym nasieniu odsetek plemników barwiących się jako żywe (SYBR-14) wynosił 97 ± 3% (rys. 1). Statystycznie istotny spadek integralności błony plazmatycznej stwierdzono po 48 h przechowywania w 8°C, podczas gdy w temperaturze 20°C już po 4 h 100% plemników wybarwiało się jako martwe (jodek propydyny). Nie stwierdzono zależności pomiędzy odsetkiem plemników barwiących się jako żywe a odsetkiem plemników ruchliwych i procentem zapłodnionych komórek jajowych.

Słowa kluczowe

EN

fluorescence method; plasma membrane; viability; zebra mussel; change; Dreissena polymorpha; spermatozoon; vitality; sperm

• Wydawca: -
• Czasopismo: Archives of Polish Fisheries
• Rocznik: 2004
• Tom: 12
• Numer: 1
• Opis fizyczny: p.23-30,fig.,ref

Twórcy

autor

Bialkowska J

• Department of Evolutionary Ecology, University of Warmia and Mazury, Oczapowskiego 5, 10-957 Olsztyn, Poland

autor

Demianowicz W

• Molecular Andrology Group, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences Olgsztyn, Poland

autor

Glogowski J

• Molecular Andrology Group, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences Olgsztyn, Poland

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