A diadenosine 5',5''-P1P4 tetraphosphate [Ap4A] hydrolase from Arabidopsis thaliana that is activated preferentially by Mn2plus ions

• Autorzy: Szurmak B. , Wyslouch-Cieszynska A. , Wszelaka-Rylik M. , Bal W. , Dobrzanska M.
• Języki publikacji: EN

Abstrakty

EN

Asymmetrical diadenosine 5',5''-P 1P 4 tetraphosphate (Ap4A) hydrolases are key enzymes controlling the in vivo concentration of Ap4A - an important signaling molecule involved in regulation of DNA replication and repair, signaling in stress response and apoptosis. Sequence homologies indicate that the genome of the model plant Arabidopsis thaliana contains at least three open reading frames encoding presumptive Ap4A hydrolases: At1g30110, At3g10620, and At5g06340. In this work we present efficient overexpression and detailed biochemical characteristics of the AtNUDX25 protein encoded by the At1g30110 gene. Aided by the determination of the binding constants of Mn(Ap4A) and Mg(Ap4A) complexes using isothermal titration calorimetry (ITC) we show that AtNUDX25 preferentially hydrolyzes Ap4A in the form of a Mn 2+ complex.

Słowa kluczowe

EN

signalling; stress response; apoptosis; DNA replication; manganese; diadenosine 5',5''-P1P4 tetraphosphate; Arabidopsis thaliana; Ap4A hydrolase

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2008
• Tom: 55
• Numer: 1
• Opis fizyczny: p.151-160,fig.,ref.

Twórcy

autor

Szurmak B.

• Polish Academy of Sciences, A.Pawinskiego 5A, 02-106 Warsaw, Poland

autor

Wyslouch-Cieszynska A.

autor

Wszelaka-Rylik M.

autor

Bal W.

autor

Dobrzanska M.

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