Application of DNA markers to estimate genetic diversity of Mycobacterium tuberculosis strains

• Autorzy: Korzekwa K , Polok K. , Zielinski R.
• Języki publikacji: EN

Abstrakty

EN

The obligatory human pathogen, Mycobacterium tuberculosis, is the most important etiological factor of tuberculosis. Unfortunately, there is little information about genetic diversity of this pathogen. The main aim of this research was the estimation of genetic diversity of M. tuberculosis on the basis of various categories of DNA markers. The genome of 32 strains were scanned by DNA markers such RAPD, IS6110 and catalase-peroxidase katG gene. All 162 identified loci were polymorphic. The genetic diversity coefficient (HT) of M. tuberculosis was 0.32 for RAPD and 0.27 for IS6110. There were 14 alleles in katG gene. All strains were characterised by the individual molecular pattern. Genetic similarity varied from 0.13 to 0.94 (RAPD markers) and from 0 to 1 for (IS6110). M. tuberculosis strains did not represent a clonal structure, single source of transmission and epidemiological relationships as well. The applied DNA markers proved to be highly efficient for analysis of genetic structure of M. tuberculosis.

Słowa kluczowe

EN

human disease; human pathogen; application; Mycobacterium tuberculosis; DNA marker; microbiology; etiological factor; genetic diversity

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2006
• Tom: 55
• Numer: 1
• Opis fizyczny: p.19-24,fig.,ref.

Twórcy

autor

Korzekwa K

• University of Warmia and Mazury in Olsztyn, Prawochenskiego street 1, 10-720 Olsztyn, Poland

autor

Polok K.

autor

Zielinski R.

Bibliografia

• Blackall P.J., N. Fegan, G.I.T. Chew and D.J. Hampson. 1998. Population structure and diversity of avian isolates of Pasteurella multocida from Australia. Microbiology 144: 279-289.
• Chen D.-H. and P.C. Ronald. 1999. A rapid DNA minipreparation method suitable for AFLP and other PCR applications. Plant Mol. Biol. Rep. 17: 53-57.
• Cole T.S. 1999. Learning from the genome sequence of Mycobacterium tuberculosis H37Rv. FEBS Lett. 452: 7-10.
• Fabre M., J.-L. Koeck, P. Le Fléche, F. Simon, V. Hervé, G. Vergnaud and C. Pourcel. 2004. High genetic diversity revealed by variable-number tandem repeat genotyping and analysis of hsp65 gene polymorphism in a large collection of"Mycobacterium canettii" strains indicates that the M. tuberculosis complex is a recently emerged clone of "M canettii". J. Clin. Microb. 42: 3248-3255.
• Farfán M., D. Minana, M.C. Fuste and J.G. Loren. 2000. Genetic relationships between clinical and environmental Vibrio cholerae isolates based on multilocus enzyme electrophoresis. Microbiology 146: 2613-2626.
• Feizabadi M.M., I.D. Robertson, D.V. Cousins, D.J. Dawson and D.J. Hampson. 1997. Use of multilocus enzyme electrophoresis to examine genetic relationships amongst isolates of Mycobacterium intracellular and related species. Microbiology 143: 1461-1469.
• Gao Q., K.E. Kripke, A.J. Saldanha, W. Yan, S. Holmes and P.M. Small. 2005. Gene expression diversity among Mycobacterium tuberculosis clinical isolates. Microbiology 151: 5-14.
• Gordon D.M. 1997. The genetic structure of Escherichia coli population in feral house mice. Microbiology, 143: 2039-2045.
• Gutacker M., C. Valsangiacomo and J-C. Piffaretti. 2000. Identification of two genetic groups in Bacteroides fragilis by multilocus enzyme electrophoresis: distribution of antibiotic resistance (cfiA, cepA) and enterotoxin (bft) encoding genes. Microbiology 146: 1241-1254.
• Hatfull G.F. and W.R. Jacobs. 2000. Molecular genetics of mycobacteria. ASM Press. Washington.
• Heym B., P.M. Alzari, N. Honore and S.T. Cole. 1995. Missense mutations in the catalase-peroxidase gene, katG, are associated with isoniazid resistance in Mycobacterium tuberculosis. Mol. Microbiol. 15: 235-245.
• Jou R., H.-Y. Chen, C.-Y. Chiang, M.-C. Yu and I.-J. Su. 2005. Genetic diversity of multidrug-resistant Mycobacterium tuberculosis isolates and identification of 11 novel rpoB alleles in Taiwan. J. Clin. Microb. 43: 1390-1394.
• Kaduma E., T.D. McHugh and S.H. Gillespie. 2003. Molecular methods for Mycobacterium tuberculosis strain typing: a user's guide. J. Appl. Microbiol. 94: 781-791.
• Korzekwa K. 2004. Ph.D. thesis. Warmia and Mazury University, Olsztyn.
• Kulaga S., M. Behr, D. Nguyen, J. Brinkman, J. Westley, D. Menzies, P. Brassard, T. Tannenbaum, L. Thibert, J.-F. Boivin, L. Joseph and K. Schwartzman. 2004. Diversity of Mycobacterium tuberculosis isolates in an immigrant population: evidence against a founder effect. Am. J. Epidemiol. 159: 507-513.
• Lewandowska K. 2001. Ph.D. thesis. Warmia and Mazury University, Olsztyn.
• Majewski J., P. Zawadzki, P. Pickerill, F.M. Cohan and C.G. Dowson. 2000. Barriers to genetic exchange between bacterial species: Srteptococcus pneumoniae transformation. J. Bacteriol. 182: 1016-1023.
• Meunier J.R. and P.A. Grimont. 1993. Factors affecting reproducibility of random amplified polymorphic DNA fingerprinting. Res. Microbiol. 144: 373-379.
• Nei M. and S. Kumar. 2000. Molecular Evolution and Phylogenetics. Oxford Univ. Press, New York.
• Ross B.C. and B. Dwyer. 1993. Rapid, simple method for typing isolates of Mycobacterium tuberculosis by using the polymerase chain reaction. J. Clin. Microbiol. 31: 329-334.
• Saint-Joanis B., H. Souchon, M. Wilming, K. Johnsson, P.M. Alzari and S.T. Cole. 1999. Use of site-directed mutagenesis to probe the structure, function and isoniasid activation of catalase/peroxidase, KatG, from Mycobacterium tuberculosis. Biochem. J. 338: 753-760.
• Suerbaum S., M. Lohrengel, A. Sonnevend, F. Ruberg and M. Kist. 2001. Allelic diversity and recombination in Campylobacter jejuni. J. Bacteriol. 183: 2553-2559.
• Tazi L., J. El Baghdadi, S. Lesjean, C. Locht, P. Supply, M. Tibayrenc and A.-L. Banuls. 2004. Genetic diversity and population structure of Mycobacterium tuberculosis in Casablanca, a Moroccan city with high incidence of tuberculosis. J. Clin. Microbiol. 42: 461-466.
• Williams J.G., A.R. Kubelik, K.J. Livak, J.A. Rafalski and S.V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res. 18: 6531-6535.
• Woodward J.M., I.D. Connaughton, V.A. Fahy, A.J. Lymbery and D.J. Hampson. 1993. Clonal analysis of Escherichia coli of serogroups 09, O20, and O101 isolated from Australian pigs with neonatal diarrhea: J. Clin. Microbiol. 31:1185-1188.
• World Health Organization. 2005. Global tuberculosis control: surveillance, planning, financing. WHO report 2005. Geneva.
• Yeh F., R. Yang and T. Boyle. 2000. Popgene version 1.32: A Microsoft Window-based freeware for population genetic analysis. Disp. in: http://www.ualberta.ca/~fyeh/info.htm. Accessed: 20 Nov. 2002.
• Zervakis G.I., G. Venturella and K. Papadopoulou. 2001. Genetic polymorphism and taxonomic infrastructure of the Pleurotus eryngii species-complex as determined by RAPD analysis, isozyme profiles and ecomorphological characters. Microbiology 147: 3183-3194.