DDT- and DDE-induced disruption of ovarian steroidogenesis in prepubertal porcine ovarian follicles: a possible interaction with the main steroidogenic enzymes and estrogen receptor ß

• Autorzy: Wojtowicz A.K. , Kajta M. , Gregoraszczuk E.L.
• Języki publikacji: EN

Abstrakty

EN

We evaluated impact of DDT isomers, o, p'- DDT [1, 1-dichloro-2, 2-bis (p, p'-chlorophenyl) ethylene] and p, p'-DDT [1, 1, 1-trichloro-2, 2-bis (p-chlorophenyl) ethane], and their metabolites, o, p'-DDE and p, p'-DDE, on ovarian steroidogenesis. All these compounds, except for p, p'-DDT, demonstrated estrogenic effects on steroid secretion in co-cultures of porcine prepubertal granulosa and theca cells. p,p'-DDT decreased progesterone and estradiol release, which was reversed by the addition of testosterone. In contrast, o, p'-DDT inhibited progesterone secretion with parallel stimulation of basal and testosterone-stimulated estradiol release. DDEs stimulated progesterone and estradiol secretion. The fluorometric assay confirmed that p,p'-DDE, o,p'-DDT, and o,p'-DDE stimulated aromatase activity. Western blots indicated that o,p-DDT and o,p'-DDE diminished the expression of estrogen receptor ß (ERß). This study demonstrated the isomer-dependent action of DDT in pig ovarian cells. We propose that DDT could disrupt ovarian steroidogenesis either by interfering with main steroidogenic enzymes or affecting ERß.

Słowa kluczowe

EN

1,1-dichloro-2,2-bis [p,p'-chlorophenyl] ethylene; prepubertal ovarian follicle; 1,1,1-trichloro-2,2-bis [p-chlorophenyl] ethane; estrogen receptor beta; ovarian steroidogenesis; organochlorine pesticide; estrogen receptor; steroidogenesis; aromatase; ovarian follicle; dichlorodiphenyltrichloroethane

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2007
• Tom: 58
• Numer: 4
• Opis fizyczny: p.873-885,fig.,ref.

Twórcy

autor

Wojtowicz A.K.

• Jagielonian University, Ingardena 6, 30-060 Krakow, Poland

autor

Kajta M.

autor

Gregoraszczuk E.L.