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2007 | 51 | 3 |

Tytuł artykułu

Duplex PCR assay for detection and differentiation of pathogenic and vaccine strains of MDV serotype 1

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
To increase the diagnostic capacity of PCR, duplex PCR for the detection of the Meq gene and 132 bp sequence of Marek's disease virus (MDV), serotype 1 has been developed. The reaction enabled the differentiation of vaccine and field strains of MDV among serotype 1 of the virus. Additionally, it gave preliminary information about eventual vaccination with the Rispens strain.

Wydawca

-

Rocznik

Tom

51

Numer

3

Opis fizyczny

p.331-335,fig.,ref.

Twórcy

autor
  • National Veterinary Research Institute, 24-100 Pulawy, Poland
autor

Bibliografia

  • 1. Baigent S., Smith L.P., Nair V.K., Currie R.J.W.: Vaccinal control of Marek's disease: current challenges, future strategies to maximize protection. Vet Immunol Immunopathol 2006, 112, 78-86.
  • 2. Baigent S., Smith L.P., Currie R.J.W., Nair V.K.: Replication kinetics of Marek's disease vaccine virus in feathers and lymphoid tissues using PCR and virus isolation. J Gen Virol 2005, 86, 2989-2998.
  • 3. Becker Y., Asher Y., Tabor E., Davidson I., Malkinson M., Weisman Y.: Polymerase chain reaction for differentiation between pathogenic and non-pathogenic serotype 1 Marek's disease virus (MDV) and vaccine viruses of MDV - serotype 2 and 3. J Virol Methods 1992, 40, 307-322.
  • 4. Becker Y., Tabor E., Asher Y., Davidson I., Malkinson M., Witter R. L.: PCR detection of amplified 132 bp repeats in Marek's disease virus type 1 (MDV-1) DNA can serve as an indicator for critical genomic rearrangement leading to the attenuation of virus virulence. Virus Genes 1992, 3, 277-287.
  • 5. Burges S.C., Davison T.F.: A quantitative duplex PCR technique for measuring amounts of cell-associated Marek's disease virus: differences in two populations of lymphoma cells. J Virol Methods 1999, 82, 27-37.
  • 6. Chang K.S., Ohashi K., Onuma M.: Diversity (Polymorphism) of the meq gene in the attenuated Marek's disease virus (MDV) serotype 1 and MDV T- transformed cell lines. J Vet Med Sci 2002, 64, 1097- 1101.
  • 7. Elnifro E.M., Ashshi A.M., Cooper R.J., Klapper P.E.: Multiplex PCR: Optimization and application in diagnostic virology. Clin Microbiol Rev 2000, 13, 559- 570.
  • 8. Henegariu O., Heerema N.A., Dlouhy S.R., Vance G.H., Vogt P.H.: Multiplex PCR: Critical parameters and step- by-step protocol. Biotechniques 1997, 23, 504-511.
  • 9. Kozdruń W., Samorek-Salamonowicz S., Czekaj H., Król K: Elaboration of multi PCR for detection of Marek's disease virus strains. Medycyna Wet 2005, 61, 711-714.
  • 10. Król K., Samorek-Salamonowicz E., Kozdruń W.: Molecular characteristic of Marek's disease virus genes and peptides. Post Mikrobiol 2005, 44, 351-356.
  • 11. Niikura M., Dodgson J. B., Cheng H. H.: Stability of Marek's disease virus 132-bp repeats during serial in vitro passages. Arch Virol 2006, 151, 1431-1438.
  • 12. Nair V., Kung H.J.: Marek's disease virus oncogenicity: molecular mechanisms. In: Marek's disease an evolving problem, edited by F. Davidson, V. Nair (Elsevier Academic Press), 2004, pp. 32-47.
  • 13. Osterrieder K., Vautherot J.F.: The genome content of Marek's disease-like viruses. In: Marek's disease an evolving problem, edited by F. Davidson, V. Nair (Elsevier Academic Press), 2004, pp. 17-29.
  • 14. Witter R.L., Schat K.A.: Marek's disease. Diseases of Poultry. XI ed., Iowa State Press, A Blackwell Publishing Company, 2003, pp.407-465.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-fc18719a-a786-4932-9921-d9134d8e6e3b
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