Search for variability among mastreviruses showing symptoms of maize streak virus from different regions of Nigeria by polymerase chain reactions

• Autorzy: Oluwafemi S , Thottappilly G. , Alegbejo M.D.

Warianty tytułu

PL

Badania nad zroznicowaniem pomiedzy masterwirusami powodujacymi objawy chorobowe na roslinach z roznych regionow Nigerii za pomoca metod PCR

• Języki publikacji: EN

Abstrakty

EN

Field surveys were undertaken in 1997-1999 across five ecological zones in Nigeria to collect grass species showing symptoms of Maize streak virus (MSV) (Genus Mastrevirus: Family Geminiviridae). Apart from maize (Zea mays L.), 15 grass species were found with symptoms similar to MSV. These hosts showed two types of symptoms viz: mild or severe. Polymerase chain reaction (PCR) and immunocapture IC-PCR were used to diagnose the mastreviruse. Variation among the isolates is illustrated in relationship dendograms. The PCR dendogram showed 35-85% variation among the isolates while the IC-PCR dendogram showed 55-85%. The PCR dendogram clusters the isolates essentially along symptom severity. Isolates that were most distantly related to a standard MSV strain showed mild symptoms in their grass hosts. Simple PCR protocols could help in studying diversity among grass Mastreviruses where facilities for genome sequencing are not available.

PL

W latach 1997-1999 dokonano przeglądu pól w pięciu strefach ekologicznych Nigerii w celu zebrania gatunków traw wykazujących objawy powodowane przez Maize starek virus (MSV, Rodzaj Mastervirus: Rodzina Geminiviridae). Oprócz kukurydzy (Zea mays L.) znaleziono 15 innych gatunków traw mających objawy podobne do zakażenia MSV. Żywiciele wykazywali dwa typy objawów, mianowicie średnie i poważne. W celu wykrycia masterwirusów użyto metod PCR (polymerase chain reaction) i IC-PCR (immunocapture polymerase chain reaction). Zróżnicowanie pomiędzy poszczególnymi izolatami ilustrują dendrogramy. Dendrogram PCR wykazuje 35-85% różnicę, natomiast dendrogram IC-PCR 55-85%. Dendrogram PCR zasadniczo grupuje izolaty pod względem nasilenia objawów. Izolaty, które były najmniej spokrewnione z podstawową linią MSV wykazywały średnie objawy na trawach żywicielskich. Nieskomplikowana procedura PCR może być pomocna w badaniach nad zróżnicowaniem masterwirusów w przypadkach, w których sekwencja genomu nie jest dostępna.

Słowa kluczowe

EN

plant protection; plant disease; variability; mastrevirus; disease symptom; maize streak virus; different region; region; Nigeria; polymerase chain reaction; isolation; Zea mays; maize; Gramineae; diversity; plant species; grass

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2008
• Tom: 48
• Numer: 4
• Opis fizyczny: p.529-539,fig.,ref.

Twórcy

autor

Oluwafemi S

• International Institute of Tropical Agriculture, Oyo Road, P.M.B. 5320, Ibadan, Nigeria

autor

Thottappilly G.

autor

Alegbejo M.D.

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