Morphological alterations in the seminiferous tubules of adult Wistar rats: the effects of prenatal ethanol exposure

• Autorzy: Fakoya F A , Caxton-Martins E.A.
• Języki publikacji: EN

Abstrakty

EN

This study presents the effects of prenatal ethanol exposure on the morphology of the seminiferous tubules of the testes in the adult male rat. Timed-pregnant adult female Wistar rats (average weight 200 g) were given daily intragastric intubation of 5.8 g/kg ethanol between gestation days 9 and 12. Pair-fed and ad lib-fed animals served as controls. The pups were weighed at birth and weaned at 30 days. At 42 days of age the male offspring (n = 10) from each group were anaesthetised and the testes removed and weighed. Another set of male rats from each group (n = 6), were anaesthetised, whole body perfused and the testes removed and processed for paraffin embedding. Sections were subjected to morphological analysis and morphometric measurements based on computerised techniques following haematoxylin and eosin, PAS and reticulin staining. The results demonstrated that prenatal ethanol exposure induced persistent growth retardation and a 66% reduction in testicular weight and severely altered the morphology of the seminiferous tubules of adult male rats, causing a reduction in the cross-sectional area of the tubules by 18%, germinal epithelium thickness by 21% (p < 0.001) and an inhibition of spermatogenesis. The study showed the absence of reticulin fibres in the peritubular tissue of seminiferous tubules of prenatal ethanol-exposed adult male rats. The results imply that damage following prenatal ethanol exposure occurs irreversibly in utero and persists into adulthood in the exposed animals, which may have implications for male fertility.

Słowa kluczowe

EN

pregnancy; Wistar rat; male; ethanol consumption; fertility; fetal alcohol syndrome; prenatal ethanol exposure; reticulin; rat; germinal epithelium

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2004
• Tom: 63
• Numer: 2
• Opis fizyczny: p.195-202,fig.,ref.

Twórcy

autor

Fakoya F A

• Obafemi Awolowo University, Ile-Ife, Nigeria

autor

Caxton-Martins E.A.

Bibliografia

• 1. Blanchard BA, Hannigan JH (1994) Prenatal ethanol exposure: effects on androgen and nonandrogen dependent behaviours and on gonadal development in male rats. Neurotoxicol Teratol, 16: 31–39.
• 2. Chandhuri JD (2000) An analysis of the teratogenic effects that could possibly be due to alcohol consumption by pregnant mothers. Indian J Med Sci, 54: 425–431.
• 3. Clarren SK, Smith DW (1978) The fetal alcohol syndrome. N Engl J Med, 298: 1063–1067.
• 4. Dahlgren IL, Matuszczyk JV, Hard E (1991) Sexual orientation in male rats prenatally exposed to ethanol. Neurotoxicol Teratol, 13: 267–269.
• 5. El-Sokkary GH (2001) Quantitative study on the effects of chronic ethanol administration on the testis of adult male rat. Neuroendocrinol Lett, 22: 93–99.
• 6. Gelly JL, Richoux JP, Leheup BP, Grignon G (1989) Immunolocalization of type IV collagen and laminin during rat gonadal morphogenesis and postnatal development of the testis and epididymis. Histochemistry, 93: 31–37.
• 7. Gordon H, Sweet HH (1936) A simple method for the silver impregnation of reticulin. Am J Pathol, 12: 545–551.
• 8. Guerri C (1998) Neuroanatomical and neurophysiological mechanisms involved in central nervous system dysfunctions induced by prenatal alcohol exposure. Alcohol Clin Exp Res, 22: 304–312.
• 9. Guerri C, Esquifino A, Sanchis R, Grisolia S (1984) Growth, enzymes and hormonal changes in offspring of alcohol-fed rats. Ciba Found Symp, 105: 85–102.
• 10. Guraya SS, Uppal J (1977) Morphological and histochemical observations on the prenatal and postnatal testes of the field rat (Millardia meltada). Andrologia, 9: 371–378.
• 11. Fakoya FA (2002a) Immunohistochemical and Neurohistological studies of the Neocortex of adult wistar rats following prenatal ethanol exposure Ph.D. Thesis, Obafemi Awolowo University, Ile-Ife, Nigeria.
• 12. Fakoya FA (2002b) Reticulin fibres in the tunica albuginea and peritubular tissue of seminiferous tubules of adult male wistar rats. Acta Histochemica, 103: 279–283.
• 13. Hadley MA, Dym M (1987) Immunocytochemistry of extracellular matrix in the lamina propria of the rat testis: electron microscopic localization. Biol Reprod, 73: 1283–1289.
• 14. Haider SG, Talati J, Servos G (1999) Ultrastructure of peritubular tissue in association with tubular hyalinization in human testis. Tissue Cell, 31: 90–98.
• 15. Hard E, Dahlgren IL, Engel J, Larsson K, Liljequist S, Lindh AS, Musi B (1984) Development of sexual behavior in prenatally ethanol-exposed rats. Drug Alcohol Depend, 14: 51–61.
• 16. Hess RA (1990) Quantitative and qualitative characteristics of the stages and transitions in the cycle of the rat’s seminiferous epithelium: Light microscopic observations of perfusion-fixed and plastic embedded testes. Biol Reprod, 43: 525–542.
• 17. Johnson L (1995) Efficiency of spermatogenesis. Microsc Res Tech, 32: 385–442.
• 18. Johnson L, Varner DD, Roberts ME, Smith TL, Keillor GE, Scrutchfield WL (2000) Efficiency of spermatogenesis: a comparative approach. Anim Reprod Sci, 60.61: 471–480.
• 19. Jones KL, Smith DW (1973) Recognition of the fetal alcohol syndrome in early infancy. Lancet, II: 999–1001.
• 20. Kelce WR, Rudeen PK, Ganjam VK (1989) Prenatal ethanol exposure alters steroidogenic enzyme activity in newborn rat testes. Alcohol Clin Exp Res, 13: 617–621.
• 21. Kerr JB (1995) Macro, micro and molecular research on spermatogenesis: the quest to understand its control. Microsc Res Tech, 32: 364–384.
• 22. Komatu S, Sakata-Haga H, Sawada K, Hisano S, Fukui Y (2001) Prenatal exposure to ethanol induces leptomeningeal heterotopia in the cerebral cortex of the rat fetus. Acta Neuropathol, 101: 22–26.
• 23. Limanowski A, Miskowiak B, Otulakowski B, Partyka M (1999) Morphometric studies on the testes of rats treated neonatally with oestrogen and subsequently with gonadotrophins and testoteron. Andrologia, 31: 225–231.
• 24. Maekawa M, Kamimura K, Nagano T (1996) Peritubular myoid cells in the testis: their structure and function. Arch Histol Cytol, 59: 1–13.
• 25. Martinez FE, Martinez M, Padovan CR, Bustos-Obregon E (2000) Morphology of testis and epididymis in an ethanol-drinking rat strain (UChA and UChB). J Submicrosc Cytol Pathol, 32: 175–184.
• 26. McGivern RF (1989) Low birthweight in rats induced by prenatal exposure to testosterone combined with alcohol, pair-feeding, or stress. Teratology, 40: 335–338.
• 27. McGivern RF, Handa RJ, Raum WJ (1998) Ethanol exposure during the last week of gestation in the rat: inhibition of the prenatal testosterone surge in males without long-term alterations in sex behaviour. Neurotoxicol Teratol, 20: 483–490.
• 28. McGivern RF, Handa RJ, Redei E (1993) Decreased postnatal testosterone surge in male rats exposed to ethanol during the last week of gestation. Alcohol Clin Exp Res, 17: 1215–1222.
• 29. McGivern RF, Raum WJ, Salido E, Redei E (1988a) Lack of prenatal testosterone surge in fetal rats exposed to alcohol: alterations in testicular morphology and physiology. Alcohol Clin Exp Res, 12: 243–247.
• 30. McGivern RF, Roselli CE, Handa RJ (1988b) Perinatal aromatase activity in male and female rats: effects of prenatal alcohol exposure. Alcohol Clin Exp Res, 12: 769–772.
• 31. Nathaniel EJ, Nathaniel DR, Mohamed SA, Nahnybida L, Nathaniel L (1986) Growth patterns of rat body, brain, and cerebellum in fetal alcohol syndrome. Exp Neurol, 93: 610–620.
• 32. Parker S, Udani M, Gavaler JS, Van Thiel DH (1984) Adverse effects of ethanol upon the sexual behaviour of male rats exposed in utero. Neurobehav Toxicol Teratol, 6: 289–293.
• 33. Pullen GL, Singh SP, Snyder AK (1988) Growth patterns of the offspring of alcohol-fed rats. Growth Dev Aging, 52: 85–89.
• 34. Rodriguez-Martinez HA, De la Luz Rosales M, Galloso de Bello L, Ruiz-Moreno JA (1973) Adequate staining of Trichomonas vaginalis by McManus’ periodic acidschiff stain. Am J Clin Pathol, 59: 741–746.
• 35. Roebuck TM, Mattson SN, Riley EP (1998) A review of the neuroanatomical findings in children with fetal alcohol syndrome or prenatal exposure to ethanol. Alcohol Clin Exp Res, 22: 339–344.
• 36. Santamaria L, Martin R, Codesal J, Ramirez R, Panigua R (1995) Immunohistochemical quantitative study of the peritubular lamina propria after induction of testicular atrophy induced by epinephrine. Int J Androl, 18: 295–306.
• 37. Sbriccoli A, Carretta D, Santarelli M, Granato A, Minciacchi D (1999) An optimised procedure for prenatal ethanol exposure with determination of its effects on central nervous system connections. Brain Res Brain Res Protoc, 3: 264–269.
• 38. Shirai T, Ikemoto I (1992) Mechanism of alcoholic testicular damage. Nippo Hinyokika Gakkai Zasshi, 83: 305–314.
• 39. Spong CY, Abebe DT, Gozes I, Brenneman DE, Hill JM (2001) Prevention of fetal demise and growth restrictions in a mouse model of fetal alcohol syndrome. J Pharmacol Exp Ther, 297: 774–779.
• 40. Udani M, Parker S, Gavaler J, Van Thiel DH (1985) Effects of in utero exposure to alcohol upon male rats. Alcohol Clin Exp Res, 9: 355–359.
• 41. Wisniewski K, Dambska M, Sher JH, Qazi Q (1983) A clinical neuropathological study of fetal alcohol syndrome. Neuropediatrics, 14: 197–201.
• 42. Xu C, Shen R (2001) Amphetamine normalises the electrical activity of dopamine neurons in the Ventral Tegmental area following prenatal ethanol exposure. J Pharmcol Exp Ther, 297: 746–752.
• 43. Zaitoun AM, Apelqvist G, Wikell C, Al-Mardini H, Bengtsson F, Record CO (1998) Quantitative studies of testicular atrophy following portacaval shunt in rats. Hepatology 28: 1461–1466.
• 44. Zhou FC, Sari Y, Zhang JK, Goodlett CR, Li T (2001) prenatal alcohol exposure retards the migration and development of serotonin neurons in fetal C57BL mice. Brain Res Dev Brain Res, 126: 147–155.
• 45. Zhu Q, Meisinger J, Emanuele NV, Emanuele MA, LaPaglia N, Van Thiel DH (2000) Ethanol exposure enhances apoptosis within the testes. Alcohol Clin Exp Res, 24: 1550–1556.
• 46. Zhu Q, Van Thiel DH, Gavaler JS (1997) Effects of ethanol on rat sertoli cell function: studies in vitro and in vivo. Alcohol Clin Exp Res, 21: 1409–1417.