Molecular cytogenetic evaluation of chromosome instability in Triticum aestivum-Secale cereale disomic addition lines

• Autorzy: Szakacs E , Molnar-Lang M
• Języki publikacji: EN

Abstrakty

EN

The genetic stability of wheat/rye ('Chinese Spring/Imperial') disomic addition lines was checked using the Feulgen method and fluorescent in situ hybridization (FISH). Feulgen staining detected varying proportions of disomic, monosomic, and telosomic plants among the progenies of the disomic addition lines. The greatest stability was observed for the 7R addition line, while the most unstable lines were those with 2R and 4R additions. Chromosome rearrangements were also detected using FISH. Based on the specific hybridization patterns of repetitive DNA probes pSc119.2 and (AAC)₅, as well as ribosomal DNA probes (5S and 45S), isochromosomes were identified in the progenies of 1R and 4R addition lines. The results draw attention to the importance of continuous cytological checks on basic genetic materials by using FISH, because this method reveals chromosome rearrangements that could not be detected either with the conventional Feulgen staining technique or with molecular markers.

Słowa kluczowe

PL

addition line; genetic stability; Triticum aestivum; Secale cereale; Feulgen method; fluorescence in situ hybridization method; molecular marker

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2010
• Tom: 51
• Numer: 2
• Opis fizyczny: p.149-152,fig.,ref.

Twórcy

autor

Szakacs E

• Agricultural Research Institute, Hungarian Academy of Sciences, H-2462 Martonvasar, P.O.Box 19, Hungary

autor

Molnar-Lang M

• Agricultural Research Institute, Hungarian Academy of Sciences, H-2462 Martonvasar, P.O.Box 19, Hungary

Bibliografia

• Anioł A, 2004. Chromosomal location of aluminium tolerance genes in rye. Plant Breeding 123: 132-136.
• Bedbrook JR, Jones J, O'Dell M, Thompson RJ, Flavell RB, 1980. A molecular description of telomeric heterochromatin in Secale species.Cell 19: 545-560.
• Cheng ZJ, Murata M, 2002. Loss of chromosomes 2R and 5RS in octoploid triticale selected for agronomic traits. Genes Gen Syst 77: 23-29.
• Chrzastek M, 2003. Cytogenetic stability of wheat lines (Triticum aestivum L.) with added and substituted chromosomes of rye (Secale cereale L.). Acta Biologica Cracoviensia Series Botanica 45: 117-126.
• Contento A, Heslop-Harrison JS, Schwarzacher T, 2005. Diversity of a major repetitive DNA sequence in diploid and polyploid Triticeae. Cytogenetic and Genome Research 109: 3-42.
• Cuadrado A, Schwarzacher T, Jouve N, 2000. Identification of different chromatin classes in wheat using in situ hybridization with simple sequence repeat oligonucleotides. Theor Appl Genet 101: 711-717.
• Driscoll C, Sears ER, 1971. Individual addition of the chromosomes of 'Imperial' rye to wheat. Agron Abst 6.
• Evans, LE, Jenkins BC, 1960. Individual Secale cereale chromosome additions to Triticum aestivum. 1. The addition of individual 'Dakold' fall rye chromosomes to 'Kharkov' winter wheat and their subsequent identification. Can J Genet Cytol 2: 205-215.
• Fra-Mon P, Salcedo G, Aragoncillo C, Garcia-Olmedo F, 1984. Chromosomal assignment of genes controlling salt-soluble proteins (albumins and globulins) in wheat and related species. Theor Appl Genet 69: 167-172.
• Gallego FJ, López-Solanilla E, Figueiras AM, Benito C, 1998. Chromosomal location of PCR fragments as a source of DNA markers linked to aluminium tolerance genes in rye. Theor Appl Genet 96: 426-434.
• Gerlach WL, Bedbrook JL, 1979. Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Research 7: 1869-1885.
• Gerlach WL, Dyer TA, 1980. Sequence organization of the repeating units in the nucleus of wheat which contain 5S rRNA genes. Nucleic Acids Research 8: 4851-4865.
• Hull G, Sabelli PA, Shewry PR, 1992. Restriction fragment analysis of the secalin loci of rye. Biochemical Genetics 30: 85-97.
• Jiang J, Friebe B, Gill BS, 1994. Chromosome painting of'Amigo' wheat. Theor Appl Genet 8: 811-813.
• Jianzhong L, Yujing L, Yiping T, Jianwei G, Bin L, Jiyun L, Zhensheng L, 2001. Chromosomal location of genes conferring the tolerance to Pi starvation stress and acid phosphatase (APase) secretion in the genome of rye (Secale L.). Plant and Soil 237: 267-274.
• Line G, Friebe BR, Kynast RG, Molnár-Láng M, Kőszegi B, Sutka J, Gill BS, 1999. Molecular cytogenetic analysis of Aegilops cylindrica host. Genome 42: 497-503.
• Lukaszewski AJ, Gustafson JP, 1983. Translocations and modifications of chromosomes in triticale × wheat hybrids. Theor Appl Genet 64: 239-248.
• Minelli S, Ceccarelli M, Mariani M, De Pace C, Cionini PG, 2005. Cytogenetics of Triticum × Dasypyrum hybrids and derived lines. Cytogenet Genome Res. 109: 385-392.
• Molnár-Láng M, Novotny C, Line G, Nagy ED, 2005. Changes in the meiotic pairing behaviour of a winter wheat-winter barley hybrid maintained for a long term in tissue culture, and tracing the barley chromatin in the progeny using GISH and SSR markers. Plant Breeding 124: 247-252.
• O'Mara JG, 1940. Cytogenetic studies on Triticale. I. A method for determining the effects of individual Secale chromosomes on Triticum. Genetics 25: 401—408.
• Riley R, Chapman V, 1958. The production and phenotypes of wheat-rye chromosome addition lines. Heredity 12: 301-315.
• Szakács E, Molnár-Láng M, 2008. Fluorescent in situ hybridization polymorphism on the 1RS chromosome arms of cultivated Secale cereale species. Cereal Res Commun 36: 247-255.
• Taketa S, Kato J, Takeda K, 1995. High crossability of wild barley (Hordeum spontaneum C. Koch) with bread wheat and the differential elimination of barley chromosomes in the hybrids. Theor Appl Genet 91: 1203-1209.
• Tang KS, Hart GE, 1975. Use of isozymes as chromosome markers in wheat-rye addition lines and in triticale. Genet Res Camb 26: 187-201.
• Taylor C, Shepherd KW, Langridge P, 1998. A molecular genetic map of the long arm of chromosome 6R of rye incorporating the cereal cyst nematode resistance gene, CreR. Theor Appl Genet 97: 1000-1012.
• Vrána J, Kubaláková M, Simková H, Cíhalíková J, Lysák MA, Dolezel J, 2000. Flow sorting of mitotic chromosomes in common wheat (Triticum aestivum L.). Genetics 156: 2033-2041.