Wykorzystanie metod ISSR i RAPD oraz analizy rodowodow do oceny podobienstwa miedzyodmianowego Avena sativa L.

• Autorzy: Paczos-Grzeda E

Warianty tytułu

EN

Avena sativa L. cultivars similarity estimation based on RAPD and ISSR methods and pedigree analysis

• Języki publikacji: PL

Abstrakty

PL

W pracy przedstawiono analizę porównawczą trzech systemów oceny podobieństwa genetycznego. Podobieństwo genetyczne 12 polskich odmian owsa zwyczajnego oszacowano na podstawie polimorfizmu markerów molekularnych: RAPD i ISSR oraz w oparciu o dane rodowodowe odmian. Średnie podobieństwo odmian określone na podstawie markerów ISSR wyniosło 0,933, zaś oszacowane w oparciu o polimorfizm markerów RAPD było nieznacznie niższe i wyniosło 0,912. Wartość współczynników rodzicielstwa określonych na podstawie danych rodowodowych była zdecydowanie niższa, aniżeli oszacowana w oparciu o polimorfizm markerów molekularnych i wyniosła średnio 0,142. Najwyższą, statystycznie istotną korelację (0,66) zaobserwowano pomiędzy matrycami indeksów podobieństwa oszacowanymi w oparciu o polimorfizm markerów ISSR i RAPD. Korelacje pomiędzy matrycą uzyskaną na podstawie rodowodów oraz matrycami uzyskanymi w oparciu o markery molekularne również były istotne, co pozwala wnioskować, że każda z wymienionych metod może być stosowana oddzielnie do oceny podobieństwa genetycznego.

EN

In the paper a comparative analysis of three genetic similarity assessement systems is presented. Genetic similarity of 12 Polish cultivars of Avena sativa L. was established based on RAPD and ISSR molecular markers and pedigree data. Mean cultivar similarity estimation based on ISSR markers was 0.933, and the one derived from RAPD was a little lower 0.912. The mean value of coefficient of parentage (COP) was considerably lower than molecular marker polimorphism-based genetic similarities and amounted to 0.142. The highest and significant correlation coefficient (0.66) was established between genetic similarities calculated from RAPD and ISSR molecular data. Coefficients of correlation between COP matrix and genetic similarity matrices derived from molecular markers were also significant. It allows a conclusion, that each method used in this studies can be apllied separately for genetic similarity assessement.

Słowa kluczowe

PL

hodowla roslin; owies zwyczajny; Avena sativa; metoda RAPD; analiza rodowodow; markery molekularne; metoda ISSR; polimorfizm molekularny; podobienstwo genetyczne; biotechnologia; odmiany roslin

EN

plant breeding; oat; Avena sativa; RAPD method; pedigree analysis; molecular marker; ISSR technique; molecular polymorphism; genetic similarity; biotechnology; plant cultivar

• Wydawca: -
• Czasopismo: Zeszyty Problemowe Postępów Nauk Rolniczych
• Rocznik: 2007
• Tom: 517
• Numer: 2
• Opis fizyczny: s.547-558,rys.,fot.,tab.,bibliogr.

Twórcy

autor

Paczos-Grzeda E

• Instytut Genetyki, Hodowli i Biotechnologii Roslin, Akademia Rolnicza, ul.Akademicka 15, 20-934 Lublin

Bibliografia

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