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2006 | 47 | 1 |

Tytuł artykułu

Polymorphism of beta-lactoglobulin gene in Indian goats and its effect on milk yield

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Polymorphism in the β-LG gene in the Indian goat was investigated by the SDS-PAGE and PCR-RFLP method. SDS-PAGE was carried out in 1098 samples belonging to 8 different breeds of Indian goats. The electrophoretic pattern in the β-LG locus showed the presence of AA and AB genotypes with frequencies of 0.81 and 0.19,0.89 and 0.11, 0.50 and 0.50, 0.80 and 0.20, 0.84 and 0.16, 1.00 and 0.00, 0.98 and 0.02 and 0.950 and 0.050 in Jamunapari, Barbari, Marwari, Sirohi, Jakhrana, Beetal, Local UP and Local MP goats, respectively. A total of 358 individuals belonging to 13 different genetic groups were analyzed by the PCR-RFLP method. The amplified product was observed as 426 bp and the restriction digestion with SacII revealed three genotypes, namely S₁S₁, S₁S₂ and S₂S₂ at the β-LG locus. The frequency of the S₂S₂ genotype ranged from 0.42 to 1.00 in the population. An analysis was carried out in Jamunapari and Barbari goats to observe the effect of the β-LG genotype on 90-day milk yield. Least squares analysis of data showed that β-LG AA animals had higher milk yield than the β-LG AB genotype in both breeds (P < 0.01).

Wydawca

-

Rocznik

Tom

47

Numer

1

Opis fizyczny

p.49-53,fig.,ref.

Twórcy

autor
  • Genetics and Breeding Division, CIRG, Makhdoom, Farah, Mathura-281122, UP, India
autor
  • Genetics and Breeding Division, CIRG, Makhdoom, Farah, Mathura-281122, UP, India
autor
  • Genetics and Breeding Division, CIRG, Makhdoom, Farah, Mathura-281122, UP, India

Bibliografia

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  • Erhardt G, 1989. Evidence for a third allele at the beta lactoglobulin locus of sheep milk and its occurrence in different breeds. Anim Genet 20: 197-204.
  • Folch JM, Dove P, Medrano JF, 1999. Differential expression of β-lactoglobulin A and B promoter variants is transciently transfected HC11 cell. J Dairy Res 66: 537-544.
  • Grosclaude F, Mahe M F, Brignon G, Di-Stasio L, Jeunet R, 1987. A Mendelian polymorphism underlying quantitative variations of goat αS1-casein, Genet Sci Evol 19: 399-412.
  • Grosclaude F, Ricordeau G, Martin P, Remuef F, Vassal L, Bouillon J, 1994. Du gene au fromagee: le polymorphismie de la caseine α-S1 caprine, ses effets, son evolution. INRA Prod Anim 7: 3-19.
  • Hambling SG, McAlpine AS, Sawyer L, 1992. β-lactoglobulin, In: Fox PF, ed. Advanced Dairy Chemistry. Vol. 1. Proteins, London. Elsevier Appl Sci: 141-190.
  • Harvey WR, 1990. User’s guide for LSMLMW PC-2version mixed model least squares maximum likelihood computer programme, Minneograph Columbus, Ohio, USA.
  • Medrano JF, Sharrow L, 1989. Milk protein typing of bovine mammary gland tissue used to generate a complementary deoxyribonucleic acid library. J Dairy Sci 72:3190-3196.
  • Moili B, Pilla F, Tripaldi C, 1998. Detection of milk protein genetic polymorphism in order to improve dairy traits in sheep and goats: A review. Small Rum Res 27: 185-195.
  • Ng-Kwai-Hang KF, 1998. Genetic polymorphism of milk proteins: relationships with production traits, milk composition and technological properties. Can J Anim Sci 78: 131-147.
  • Pena RN, Sanchez A, Folch JM, 2000. Characterization of genetic polymorphism in the goat beta-lactoglobulin gene. J Dairy Res 67: 217-224.
  • Prakash Kumar, Rout PK, Shukla SN, Mandai A, Roy R, 2002. Genetics of milk protein variants in different Indian Goats. Xth International Congress Asian-Australian Association of Animal Production Societies (AAAP), Sept. 22-24, New Delhi: 168.
  • Thangaraj K, Joshi MB, Reddy AG, Gupta NJ, Chakraborthy B, Singh L, 2002. CAG repeat expansion in androgen receptor gene is not associated with male infertility in Indian populations. J Androl 23: 815-818.

Typ dokumentu

Bibliografia

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