The transcript expression profile of the leptin receptor-coding gene assayed with the oligonucleotide microarray technique - could this be an anorexia nervosa marker?

• Autorzy: Janas-Kozik M , Mazurek U. , Krupka-Matuszczyk I. , Stachowicz M. , Glogowska-Ligus J. , Wilczok T.
• Języki publikacji: EN

Abstrakty

EN

Anorexia nervosa is a serious eating disorder with the highest mortality rate of any psychiatric disorder. The DSM-IV classification differentiates two AN types: the restricting type (AN-R) and the binge-eating/purging type (AN-BP). Leptin (LEP) levels can be thought of as a signal to the body of its energy reserves. The leptin receptor (including all its mRNA isoforms) is expressed in many tissues. Our aim was to discover the transcript expression profile of the LEP receptor-coding gene in the peripheral blood mononuclears in AN-R and AN-BP patients. Three young women suffering from Anorexia nervosa (one with AN-BP and two with AN-R) took part in the study, along with three non-anorexic subjects as our reference group. LEP receptor gene expression was examined using the oligonucleotide microarray method (HG-U133A, Affymetrix). The results were normalized using RMAExpress. Next, the accumulation analysis method was used (clustering). Hierarchical clustering resulted in three groups of separate clusters. The first group (cluster I) consisted of AN-R patients. The next group (cluster II) consisted of reference group patients suffering from different psychic disorders not related to eating disorders. Cluster III consisted of two patients — the first with AN-BP and the second with an adaptive disorder.

Słowa kluczowe

EN

human disease; leptin receptor; patient; oligonucleotide microarray technique; gene expression; mortality; coding gene; anorexia nervosa

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2006
• Tom: 11
• Numer: 1
• Opis fizyczny: p.62-69,fig.,ref.

Twórcy

autor

Janas-Kozik M

• Medical University of Silesia, Katowice, Poland

autor

Mazurek U.

autor

Krupka-Matuszczyk I.

autor

Stachowicz M.

autor

Glogowska-Ligus J.

autor

Wilczok T.

Bibliografia

• 1. Rajewski, A. Eating disorders. In: Psychiatry of children and youth (Namyslowska, I. Ed.), PZWL, Warszawa (2004) 247-266.
• 2. Bratta, M. Leptin-from a signal of adiposity to a hormone mediator in peripheral tissues. Med. Sci. Monit. 8 (2002) 282-292.
• 3. Pico, C., Oliver, P., Sanchez, J. and Palou, A. Gastric leptin: a putative role in the short-term regulation of food intake. Br. J. Nutr. 90 (2003) 735-741.
• 4. Widjaja, A., Kielstein, J.T., Horn, R., Mühlen, A., Kliem, V. and Brabant, G. Free serum leptin but not bound leptin concentrations are elevated in patients with end-stage renal disease. Nephrol. Dial. Transplant. 15 (2000) 846-850.
• 5. Meier, A. and Gressner, A.M. Endocrine regulation of energy metabolism: review of pathobiochemical and clinical chemical aspects of leptin, ghrelin, adiponectin and resistin. Clin. Chem. 50 (2004) 1511-1525.
• 6. Quinton, N.D., Meechan, D.W., Brown, K., Eastwood, H., Blakemore, A.L. Single nucleotide polymorphisms in the leptin receptor gene: studies in Anorexia nervosa. Psychiatr. Genet. 14 (2004) 191-194.
• 7. Tsiotra, P.C., Pappa, V., Raphis, S.A. and Tsigos, C. Expression of the long and short leptin receptor isoforms in peripheral blood mononuclear cells: implications for leptin’s actions. Metabolism 49 (2000) 1537-1541.
• 8. Burguera, B., Couce, M.E., Long, J., Lamsam, J., Laakso, K., Jensen, M.D., Parisi, J.E. and Lloyd, R.V. The long form of leptin receptor (Ob-Rb) is widely expressed in the human brain. Neuroendocrinology 71 (2000) 187- 195.
• 9. ICD-10 classification of mental and behavioral disorders. Research diagnostic criteria. Medical University Publishing House “Vesalius”. The Institute of Psychiatry and Neurology. Kraków-Warszawa 1998, 106.
• 10. American Psychiatric Association, 1994. Diagnostic and Statistical Manual of Mental Disorders, 4th ed. (DSM-IV).
• 11. Chomczynski, P. and Sacchi, N. Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162 (1987) 156-159.
• 12. (http://www.affymetrix.com/index.affx).
• 13. (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=nucleotide&cmd=search &term=LEPR).