Responses of mammary amino acid metabolism and aminopeptidase N gene expression to duodenal soybean small peptides and infusion of free amino acids in lactating goats

• Autorzy: Liu H. , Wang L. , Wang L.-B. , Li S.-L. , Cao Z.-J.
• Języki publikacji: EN

Abstrakty

EN

The duodenal perfusion technique was used to determine whether small peptides were more effective than free amino acids (FAA) for milk protein synthesis and to investigate the effects of infusing soyabean small peptides (SSP) or their FAA on the mammary amino acid (AA) metabolism of lactating goats. Six Saanen goats with silicon catheters implanted into their carotid artery and mammary veins were used in a Latin square design. Blood samples were collected from the carotid artery and mammary vein. The AA concentrations in the plasma, as well as mammary AA uptakes were monitored. The results showed that the concentrations of most essential amino acids (EAA) were significantly higher (P<0.05) in the FAA infusion treatment as compared with the SSP treatment, except for Val, Ile, and Met. The concentration of most of the non-essential amino acids (NEAA), measured after FAA infusion, was highest (P<0.05) when compared with the SSP treatment and the controls. The mammary uptake of most of EAA in the FAA infusion group was higher in comparison with the SSP infusion group. The mammary uptake to milk output ratios of EAA (except for Met) were low in the SSP treatment, compared with the FAA treatment. Significant increases in milk protein yield and milk protein content in the FAA infusion treatment were observed (P<0.05). In the SSP infusion treatment, milk yield and milk protein were numerically increased compared with the control. The abundance of aminopeptidase N (APN) mRNA in mammary tissue was 38.28 in the SSP infusion group, and 2.83, 5.28 in the control and FAA infusion treatment, respectively. In conclusion, the data suggest the FAA was more effective for milk protein synthesis, while the activity of APN was related to utilization of small peptides by the mammary gland.

Słowa kluczowe

PL

mammary metabolism; goat; lactating goat; milk protein; amino acid; aminopeptidase; gene expression; soybean small peptide; free amino acid; infusion; Saanen goat; milk yield

• Wydawca: -
• Czasopismo: Journal of Animal and Feed Sciences
• Rocznik: 2010
• Tom: 19
• Numer: 1
• Opis fizyczny: p.24-36,ref.

Twórcy

autor

Liu H.

• China Agricultural University, Beijing 100193, P.R.China

autor

Wang L.

autor

Wang L.-B.

autor

Li S.-L.

autor

Cao Z.-J.

Bibliografia

• Addison J.M.D., Matthews D.M., 1972. Evidence for active transport of the dipeptide glycylsarcosine by hamster jejunum in vitro. Clin. Sci. 43, 907-911
• Backwell F.R.C., Hipolito-Reis M., Wilson D., Bruce L.A., Buchan V., MacRae J.C., 1997. Quantification of circulating peptides and assessment of peptide uptake across the gastrointestinal tract of sheep. J. Anim. Sci. 75, 3315-3322
• Bequette B.J., Backwell F.R.C., Crompton L.A., 1998. Current concepts of amino acid and protein metabolism in the mammary gland of the lactating ruminant. J. Dairy Sci. 81, 2540-2559
• Bequette B.J., Backwell F.R.C., Kyle C.E., Crompton L.A., France J., MacRae J.C., 1999. Vascular sources of phenylalanine, tyrosine, lysine, and methionine for casein synthesis in lactating goats. J. Dairy Sci. 82, 362-377
• Bequette B.J., Backwell F.R.C., MacRae J.C., Lobley G.E., Crompton L.A., Metcalf J.A., Sutton J.D., 1996a. Effect of intravenous amino acid infusion on leucine oxidation across the mammary gland of the lactating goat. J. Dairy Sci. 79, 2217-2224
• Bequette B.J., Metcalf J.A., Wray-Cahen D., Backwell F.R.C., Sutton J.D., Lomax M.A., MacRae J.C., Lobley G.E., 1996b. Leucine and protein metabolism in the lactating dairy cow mammary gland: responses to supplemental dietary crude protein intake. J. Dairy Res. 63, 209-222
• Bruckental I., Ascarelli I., Yosif B., Alumot E., 1991. Effect of duodenal praline infusion on milk production and composition in dairy cows. Anim. Prod. 53, 299-303
• Chen H., Wong E.A., Wedd Jr. K.E., 1999. Tissue distribution of a peptide transporter mRNA in sheep, dairy cows, pigs, and chickens. J. Anim. Sci. 77, 1277-1283
• Choung J.J., Chamberlain D.G., 1993. The effects of aboamsal infusions of casein or soya-beanprotein isolate on the milk production of dairy cows in mid-lactation. Brit. J. Nutr. 69, 103-115
• Derrig R.G., Clark J.H., Davis C.L., 1974. Effects of abomasal infusion of sodium casein on milk yield, nitrogen utilization and amino acid nutrition of the dairy cow. J. Nutr. 104, 151-159
• Emmerson K.S., Phang J.M., 1993. Hydrolysis of praline dipeptides completely fulfills the praline requirement in a praline-auxotrophic Chinese hamster ovary cell line. J. Nutr. 123, 909-914
• Gilbert E.R., Wong E.A., Vaughan M., Webb Jr. K.E., 2007. Distribution and abundance of nutrient transporter mRNA in the intestinal tract of the black bear, Ursus americanus. Comp. Biochem. Physiol. Pt. B 146, 35-41
• Guinard J., Rulquin H., Verite R., 1994. Effect of graded levels of duodenal infusion of casein on mammary uptake in lactating cows. 1. Major nutrients. J. Dairy Sci. 77, 2221-2231
• Katz M., Bergman L., 1969. Simultaneous measurements of hepatic and portal venous blood flow in the sheep and dog. Amer. J. Physiol. 216, 946-952
• Koeln L.L., Schlagheck T.S., Webb Jr. K.E., 1993. Amino acid flux across the gastrointestinal tract and liver of calves. J. Dairy Sci. 76, 2275-2285
• Mabjeesh S.J., Cohen M., Gal-Garber O., Milgram J., Feuermann Y., Shamay A., 2005. Aminopeptidase N gene expression and abundance in caprine mammary gland in influenced by circulating plasma peptide. J. Dairy Sci. 88, 2055-2064
• Mabjeesh S.H., Cohen M., Gal-Garber O., Shamay A., Uni Z., 2001. Amino peptidase gene expression in caprine mammary gland: A possible role in peptide-bound amino acid uptake. J. Dairy Sci. 84, Suppl. 1, 313 (Abstr.)
• Mabjeesh S.J., Kyle C.E., MacRae J.C., Bequtte, B.J., 2000a. Lysine metabolism by the mammary gland of lactating goats at two stages of lactation. J. Dariy Sci. 83, 996-1003
• Pan Y.L., Bender P.K., Akers R.M., Webb K.G., 1996. Methionine-containing peptides can be used as methionine sources for protein accretion in cultured C2C12 and MAT-T cells. J. Nutr. 126, 232-241
• Remond D., Bernard L., Poncet C., 2000. Free and peptides amino acid net flux across the rumen and the mesenteric- and portal-drained viscera of sheep. J. Anim. Sci. 78, 1960-1972
• Roetsm E., Verbeke R., Peeters G., Axmann A., Proksch G., 1979. Metabolism of ornithine in perfused goat udder. J. Dairy Sci. 80, 2098-2103
• Sambrook J., Russell D., 2006. Molecular Cloning-Laboratory Manuals. Cold Spring Harbor Laboratory. New York, pp. 83
• SAS, 1998. SAS/SAT User’s Guide, Version 7. Statistical Analysis System Institute. Cary, NC Seal C.J., Parker D.S., 1991. Isolation and characterization of circulating low molecular weight peptides in steer, sheep and rat portal and peripheral blood. Comp. Biochem. Physiol. Pt. B 99, 679-685
• Shennan D.B., Backwell F.R., Calvert D.T., 1999. Metabolism of aminoacyi-p-nitroanilides by rat mammary tissue. Biochim. Biophys. Acta 1427, 227-235
• Shennan D.B., Calvert D.T., Backwell F.R., Boyd C.A., 1998. Peptide aminonitrogen transport by the lactating rat mammary gland. Biochim. Biophys. Acta 1373, 252-260
• Tagari H., Webb Jr. K.E., Theuer B. et al., 2008. Mammary uptake, portal-drained visceral flux, and hepatic metabolism of free and peptide-bound amino acids in cows fed steam-flaked or dryrolled sorghum grain diets. J. Dairy Sci. 91, 679-697
• Tagari H.K., Webb Jr. K.E., Theurer B. et al., 2004. Portal drained visceral flux, hepatic metabolism, and mammary uptake of free and peptide-bound amino acids and milk amino acids output in dairy cows fed diets containing corn grain steam flaked at 360 or steam rolled at 490 g/L. J. Dairy Sci. 87, 413-430
• Wang J.F., Wang Z.H., Li B., Lin X.Y., 2007. Responses of milk protein, arterial AA concentration, and mammary AA metabolism to graded abomasal casein infusion in lactating goats. Small Ruminant Res. 73, 206-215