Immunoglobulins anti-endonuclease 32 kDa from Cucurbita pepo var.patissonina affect process of DNA synthesis

• Autorzy: Rzepecki R. , Szopa J.
• Języki publikacji: EN

Abstrakty

EN

Immunoglobulins anti-endonuclease 32 kDa inhibit DNA synthesis. We observed that low concentrations of IgGs (about 50 jig IgG per 1 x 106 cell nuclei) temporary inhibit DNA synthesis. This inhibition concerns only the synthesis of DNA bound to the nuclear matrix (associated with isolated nuclear matrix). Preincubation of cell nuclei of White bush with IgG generates longer DNA fragments than in controls. Involvement of the 32 kDa endonuclease or an endonuclease-65 kDa protein complex from the nuclear matrix in replication or structural organisation of replication is considered.

Słowa kluczowe

EN

immunoglobulin; replication; DNA synthesis; White bush; nuclear matrix; endonuclease; Cucurbita pepo; plant

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1995
• Tom: 42
• Numer: 2
• Opis fizyczny: p.177-182,fig.

Twórcy

autor

Rzepecki R.

• Wroclaw University, S.Przybyszewskiego 63, 51-148 Wroclaw, Poland

autor

Szopa J.

Bibliografia

• 1. Van der Velden, H.M.W. & Wanka, F. (1987) The nuclear matrix — Its role in the spatial organization and replication of eukaryotic DNA. Mol Biol Reports 12, 69-77.
• 2. Verheijen, R., van Venroy, W. & Ramaekers, F. (1988) The nuclear matrix: structure and composition. }. Cell $ci. 90,11-36.
• 3. Zeitlin, S., Parent, A., Silverstein, S. & Efstratiadis, A. (1987) Pre-mRNA splicing and the nuclear matrix. Mol Cell Biol. 7,111-120.
• 4. Smith, H.C., Hams, S.G., Zillmann, M. & Berget, S.M. (1989) Evidence that a nuclear matrix protein participates in premessenger RNA splicing. Exp. Cell Res. 182,521-533.
• 5. Klehr, D., Maass, K. & Bode, J. (1991) Scaffold-attached regions from the human interferon p domain can be used to enhance the stable expression of genes under the control of various promoters- Biochemistry 30,1264-1270.
• 6. Wisniowska, J., Rudnicki, K, & Szopa, J. (1987) The presence of nuclease-inhibitor complex in White bush cell nuclei. Effect of phytohor- mones. /. Plant Physiol 129,407-413.
• 7. Rudnicki, K., Rzepecki, R. & Szopa, J. (1988) Rearrangement of the nuclease-inhibitor complex components within cells of White bush treated with phytohormone. /. Plant Physiol 132, 658-663.
• 8. Rzepecki, R., Bulaj, G. & Szopa, J. (1989) Endonuclease tightly associated with plant nuclear matrix affected DNA synthesis in vitro. J. Plant Physiol 134,364-369.
• 9. Rzepecki, R., Szmidzirtski, R., Bode, J. & Szopa, J. (1992) The 65 kDa protein affected endonuclease tightly associated with plant nuclear matrix. J. Plant Physiol 139,284-288.
• 10. Rzepecki, R. (1994) The complex of the endonuclease 32 kDa and 65 kDa protein fiom nuclear matrix preferentially recognize plasmid with SAR DNA element. }. Plant Physiol 144, 479-484.
• 11. Linial, M. & Shlomai, J. (1988) A unique endo­nuclease from Crithidia fasciculata which reco­gnizes a bend in the DNA helix. /. Biol Chem. 263,290-297.
• 12. Pieck, A.C.M., Van der Welden, H.M.W., Rijken, A.A.M., Neis, J.M. & Wanka, F. (1985) Protein composition of the chromosomal scaffold and interphase nuclear matrix. Chromosoma 91, 137-144.
• 13. Maniatis, T, Fritsch, E.F. & Sambrook, J. (1989) Molecular cloning. Laboratory manual Cold Spring Harbor Laboratory Press, New York.
• 14. Tsutsui, K., Tsutsui, K., Sakurai, H., Shohmori, T. & Oda, T. (1986) Levels of topoisomerase II and DNA polymerase are regulated independently in developing neuronal nuclei. Biochem. Biophys. Res. Commun. 138,1116-1122.
• 15. Smith, H.C., Puvion, E., Buchholtz, L.A. & Berezney, R. (1984) Spatial distribution of DNA loop attachment and replicational sites in the nuclear matrix. /. Cell Biol 99,1794-1802.
• 16. Rzepecki, R., Markiewicz, E. & Szopa, J. (1995) Identification of Cucurbita pepo nuclear matrixproteins binding to SAR DNA. Acta Biochim. Polon. 42,171-176.